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Molecular marker of pure white Hypsizygus marmoreus Finc-W-62 strain, its acquisition method and application

A technology of finc-w-62, white shimeji mushroom, applied in biochemical equipment and methods, determination/inspection of microorganisms, DNA preparation, etc., to achieve the effects of high accuracy, short detection time, and overcoming long time consumption

Active Publication Date: 2013-09-11
SHANGHAI FINC BIO TECH INC
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SCAR (Sequence-characterized Amplified Region) marker is a very stable molecular marker, which can be used for simple, fast and stable identification of strains. However, there is no SCAR molecular marker for pure white mushroom Finc-W-62. In order to quickly identify the pure white mushroom Finc-W-62

Method used

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  • Molecular marker of pure white Hypsizygus marmoreus Finc-W-62 strain, its acquisition method and application
  • Molecular marker of pure white Hypsizygus marmoreus Finc-W-62 strain, its acquisition method and application
  • Molecular marker of pure white Hypsizygus marmoreus Finc-W-62 strain, its acquisition method and application

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Embodiment Construction

[0019] The "H-W" in the following examples is a pure white shimeji mushroom variety that was selected and bred by Japan Beidou Co., Ltd. in 2002. It is completely white and has no bitter taste; Jiji mushroom strain, its primordium is gray, gradually turns white with the growth and development of the fruiting body, the stipe is off-white during the harvest period, and the cap is pure white; the "GC-W" strain is newly bred in Katsuragi, Japan white jade mushroom strain.

[0020] A method for obtaining a molecular marker of pure white shimeji mushroom Finc-W-62, comprising the steps of:

[0021] 1) Mycelium culture and aerial mycelium acquisition

[0022] Inoculate Finc-W-62, H-W, G-W and GC-W preserved at low temperature on PDA medium, culture at 22°C and 75% humidity for 20 days, scrape aerial mycelia, freeze at -20°C live.

[0023] 2) Strain genomic DNA extraction

[0024] Extract according to the instructions of Sangon Bioengineering (Shanghai) Co., Ltd. (hereinafter refe...

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Abstract

The invention relates to a molecular marker of a pure white Hypsizygus marmoreus Finc-W-62 strain. Its specific PCR amplification primer pair sequences are 5'-ATAAGGCGTTCCAAACTCATCTC-3' and 5'-TAAGAGACCAACTGCCCTTATCTC-3'; and the size of its DNA fragment is 1082bp. The invention also discloses an acquisition method and application of the molecular marker of the pure white Hypsizygus marmoreus Finc-W-62 strain.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker of pure white jiji mushroom Finc-W-62 strain, an obtaining method and application thereof. 【Background technique】 [0002] True Ji mushroom (Latin name Hypsizygus marmoreus), belongs to Basidiomycotina, Laminomycetes, Agaricaceae, White Mushrooms, and Amygium genus. Because it has a unique crab flavor, it is also called crab-flavored mushroom. . The initial artificial cultivation began in Japan around 1978. It is delicious, crisp and tender, and rich in nutrition. The product is also full of fragrance. True jiji mushroom is rich in vitamins and 17 kinds of amino acids, and the β-glucan extracted from the fruiting body has high anti-tumor activity. It is a low-calorie, low-fat health food. [0003] The identification of traditional edible fungi, especially for Shiji mushroom, is mainly based on the appearance of the fruiting body, antagonism test, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 刘玉
Owner SHANGHAI FINC BIO TECH INC
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