Factorization strain production method for hypsizygus marmoreus and cultivation method for hypsizygus marmoreus

A production method and technology of shimeji mushrooms are applied in the production field of edible fungi, which can solve problems such as complicated operation, and achieve the effects of improving utilization rate, avoiding the decline of sanitary conditions and high quality.

Inactive Publication Date: 2012-08-01
上海光明森源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this patent has shortened the cultivation cycle of factory-made jiji mushrooms,

Method used

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  • Factorization strain production method for hypsizygus marmoreus and cultivation method for hypsizygus marmoreus

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Experimental program
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Embodiment 1 and Embodiment 2

[0028] In the following embodiment 1 and embodiment 2:

[0029] The composition of the plate medium (weight to volume ratio g / 100mL) is: glucose 2%, potato 20%, agar 2%, and the volume is adjusted to the required volume, and the pH is 5.5-6.5.

[0030] The shake flask medium composition (weight to volume ratio g / 100mL) is: 0.4-0.8% of soybean meal powder, 0.03-0.05% of magnesium sulfate, 0.03-0.05% of potassium dihydrogen phosphate, and the volume is adjusted to the required volume, and the pH is 6.0-7.0.

[0031] The composition of the fermentation medium (weight to volume ratio g / 100mL) is: 0.4-0.8% of soybean meal powder, 0.03-0.05% of magnesium sulfate, 0.03-0.05% of dipotassium hydrogen phosphate, 0.03-0.06% of defoamer, and pH 6.0-7.0.

[0032] The composition (weight percentage) of the original seed culture medium is: 79% of sawdust, 20% of rice bran and 1% of calcium carbonate.

Embodiment 1

[0033] Embodiment 1: Insert the slanted-plane female species of jiji mushroom into a disposable plate (7-9cm in diameter) equipped with 15-20mL plate medium, and cultivate it for 12-14 days at 22-25°C in a biochemical incubator; Transfer the seed into a 500mL shake flask with 300mL shake flask culture medium, culture in a shaker at 22-25°C for 6-8 days, and the shaker speed is 150r / min; In the 300L fermenter of 250L fermentation medium, the tank temperature is maintained at 22~25° C., the ventilation pressure is maintained at 0.05~0.12MPa, and cultured for 6~8 days to obtain the fermenter liquid strain (original species).

Embodiment 2

[0034] Embodiment 2: In the disposable plate (7-9cm in diameter) that 15~20mL plate culture medium is housed in inserting the slanted-plane parent species of shimeji mushroom, cultivate 12~14 days at 22~25° C. in the biochemical incubator; Transfer the seed into a 500mL shake flask with 300mL shake flask culture medium, culture in a shaker at 22-25°C for 6-8 days, and the shaker speed is 150r / min; In a 300L fermenter with 250L fermentation medium, the temperature of the tank is maintained at 22-25°C, the ventilation pressure is maintained at 0.05-0.12MPa, and the liquid strain of the fermenter is cultivated for 6-8 days; the liquid strain of the fermenter is inoculated with a liquid inoculator Insert into a 1100cc original seed bottle with 700g of original seed compost, maintain the temperature at 22-25°C, CO 2 The concentration is controlled at 2000-4000ppm, the humidity is controlled at 60-70%, and the original species is obtained by culturing in the dark for 35 days.

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Abstract

The invention relates to a factorization strain production method for hypsizygus marmoreus and a cultivation method for the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus comprises inserting inclined plane stock culture into a flat plate to cultivate for 12-14 days at the temperature of 22 DEGC to 25 DEG C; then transferring flat plate strains into a shake flask filled with shake flask culture media to cultivate for 6-8 days at the temperature of 22 DEGC to 25 DEG C to obtain shake flask strains; then transferring the shake flask into a fermentation tank filled fermentation media, keeping temperature at 22 DEGC to 25 DEG C, maintaining ventilation pressure at 0.05-0.15MPa, and cultivating for 6-8 days to obtain fermentation tank liquid strains; inserting the fermentation tank liquid strains into a stock bottle filled with stock materials to cultivate for 35-40 days in dark to achieve stock, and picking and sterilizing to obtain factorization strains of the hypsizygus marmoreus. The factorization strain production method for the hypsizygus marmoreus has the advantages of being short in period and high in quality, can guarantee advantages of solid strains on mycelium stimulation mode of the hypsizygus marmoreus and hypsizygus marmoreus output quality and has good popularization value.

Description

technical field [0001] The invention belongs to the technical field of edible fungus production, and in particular relates to a production method of industrialized strains of shimeji mushroom and a method for cultivating jitake mushrooms using the strains produced by the method. Background technique [0002] Hypsizigus marmoreus is a rare edible fungus with crisp and tender texture, delicious taste, rich nutrition and multiple health effects, and is deeply loved by consumers. [0003] At present, there are mainly two methods of industrial strain production of shimeji mushroom at home and abroad: solid strain and liquid strain. Cao Debin made a lot of comparisons between solid strains and liquid strains, and each has its own merits. [0004] When using solid strains to produce jitake mushrooms, ring scratching is used (scratching the surrounding compost and leaving the middle part). Through cultivation control, the fruiting bodies can be in the shape of steamed buns and have...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 潘辉郭倩章芳芳周敏宗雨晨
Owner 上海光明森源生物科技有限公司
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