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Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker

A technology of shimeji mushrooms and strains, which is applied in the field of molecular biology identification of strains to achieve the effect of protecting intellectual property rights and enhancing competitiveness

Inactive Publication Date: 2010-03-31
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But its biggest inconvenience is the design of the primer sequences at both ends
Because of the relative conservation and single-copy nature of the sequences at both ends of the SSR sequence, this technique should be applied when understanding the genetic background of the species and designing corresponding primers. At present, because little is known about the molecular information of P. Using SSR method to mark and identify Pleurotus jiruji

Method used

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  • Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker
  • Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker
  • Method for identifying industrially cultivated strains of hypsizygus marmoreus by using SSR molecular marker

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Embodiment 1

[0049] 1. DNA sequence data collection of similar species

[0050] Downloaded from the Fungal Genome Project website FGP (http: / / fungalgenomics.concordia.ca) and NCBI (http: / / www.ncbi.nlm.nih.gov) the length of mushrooms and other similar species of eutrophicum greater than 150bp, 5' and 3' end 50bp does not contain (T) 5 or (A) 5 DNA sequences with close relatives such as Lentinula edodes, Pleurotus otreatus, and Coprinus cinereus.

[0051] 2. Discover SSR in DNA sequence

[0052] Use the SSR identification tool SSRIT (Simple Sequence Repeat Identification Tool) provided by the GRAMENE website to perform SSR identification on the downloaded EST sequence (http: / / www.gramene.org / db / searches / ssrtool), or use SSRHunter 1.3 software combined with manual SSR find. The identification criteria were the minimum lengths of single, di, tri, quad, penta and hexanucleotide repeat motifs were 15, 8, 5, 4, 3 and 3 times, respectively.

[0053] 3. PCR primer design

[0054] Select elig...

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Abstract

The invention discloses a method for identifying industrially cultivated strains of hypsizygus marmoreus, namely identifying industrially cultivated strains of hypsizygus marmoreus by using an SSR marker in a molecular marker method. In the method, the known DNA sequence of the close edible fungus species of the hypsizygus marmoreus is adopted, the SSR primer sequence is designed and screened and the SSR marker map is successively built, thus realizing identification of the industrially cultivated strains of hypsizygus marmoreus. The method can contribute to rapidly, accurately and economically identifying the industrially cultivated strains of hypsizygus marmoreus.

Description

technical field [0001] The invention relates to a molecular biological identification method of bacterial strains, in particular to a SSR molecular marker identification method of industrially cultivated strains of shimeji mushroom. Background technique [0002] True Ji mushroom [Hypsizygus marmoreus (Peck) Bigelow], also known as crab-flavored mushroom, seafood mushroom, Hongxi mushroom, jade mushroom, gelatinous jade mushroom, belongs to Basidiomycotina, Phytomycetes, Agaricaceae, White Mushrooms , Lipophyllum family, and Amygium genus are rare edible fungi that are crisp and tender in texture, delicious in taste, rich in nutrition, and have various health benefits, and are deeply loved by consumers. [0003] my country's scientific research institutes and edible fungus factories have developed new strains suitable for industrial cultivation, which have the characteristics of high yield, high quality and strong stress resistance. With the further expansion of the consumpt...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
Inventor 冯志勇董岩陈明杰高君辉陈辉
Owner SHANGHAI ACAD OF AGRI SCI
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