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Culture medium, cultivation medium and cultivation method of Hypsizygus marmoreus liquid strain culture

A technology of liquid strain and medium, applied in mushroom cultivation, cultivation, plant cultivation and other directions, can solve the problems of the degradation ability of the medium during the cultivation time, affecting the yield of seafood mushrooms, etc., to shorten the fruiting time, improve the process control, The effect of shortening the germinal period and

Active Publication Date: 2019-01-11
SICHUAN JUNLYU ECOLOGICAL AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during production, it was found that the culture time of seafood mushrooms inoculated with liquid strains was not shortened compared with that of seafood mushrooms inoculated with solid strains, and the degradation ability of the medium was reduced. In addition, the output of each bag of seafood mushrooms inoculated with liquid Seafood mushroom output is also lower than the seafood mushroom inoculated by solid strains, which seriously affects the output of seafood mushroom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The present embodiment provides a culture medium for the production of seafood mushroom liquid strains, including PDA culture medium, visible bottle culture medium, shake flask culture medium and fermenter culture medium, and the PDA culture medium and visible bottle culture medium include the following weight percentages Raw materials: 0.2% peptone, 20% potatoes, 1% glucose, 0.1% magnesium sulfate, 2.3% agar powder, 0.1% lignin powder, and the balance is water; the shake flask medium includes the following raw materials in weight percentage: glucose 3% , yeast extract 0.7%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B 0.001%, lignin powder 0.1%, and the balance is water; the fermenter culture medium includes the following raw materials in weight percentage: soybean meal powder 0.5% , corn flour 0.5%, yeast extract 0.1%, glucose 0.15%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, defoamer 0.015%, lignin powder 0.1%, and the bala...

Embodiment 2

[0022] The present embodiment provides a culture medium for the production of seafood mushroom liquid strains, including PDA culture medium, visible bottle culture medium, shake flask culture medium and fermenter culture medium, PDA culture medium, visible bottle culture medium, shake flask culture The percentage by weight of the lignin powder in the base and the culture medium of the fermenter is 0.05%, and the weight percentages of the remaining components and components are the same as in Example 1.

[0023] The liquid cultivar prepared by adopting the seed production medium of the present embodiment is designated as ZP-2.

Embodiment 3

[0025] The present embodiment provides a culture medium for the production of seafood mushroom liquid strains, including PDA culture medium, visible bottle culture medium, shake flask culture medium and fermenter culture medium, PDA culture medium, visible bottle culture medium, shake flask culture The percentage by weight of the lignin powder in the base and the culture medium of the fermenter is 0.2%, and the weight percentages of the other components and components are the same as in Example 1.

[0026] The liquid cultivar prepared by adopting the seed production medium of the present embodiment is designated as ZP-3.

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Abstract

The invention relates to a culture medium, a cultivation medium and a cultivation method for Hypsizygus marmoreus liquid strain cultivation. The culture medium includes a PDA medium, a visual bottle medium, a shake flask medium and a fermenter medium, wherein the shake flask medium includes the following materials of glucose, yeast extract, monopotassium phosphate, magnesium sulfate, vitamin B, lignin powder and the water, the fermenter medium includes the following materials of soybean meal, corn flour, yeast extract, glucose, magnesium sulfate, monopotassium phosphate, defoamer, lignin powder and the water, and the culture medium includes the following materials of cottonseed hulls, wood chips, corn flour, soybean meal, bran, lime, gypsum powder and the water. The culture medium is advantaged in that the cultivation time of the Hypsizygus marmoreus can be shortened, the conversion rate of the culture medium is improved, cost is reduced, and the yield is improved.

Description

technical field [0001] The invention belongs to the technical field of edible mushroom cultivation, and in particular relates to a culture medium for seafood mushroom liquid strain seed production, a cultivation medium and a cultivation method. Background technique [0002] Seafood mushroom is a kind of edible fungus with high nutritional value and medicinal value. It belongs to a strain of Shiji mushroom. to 8cm to 14cm, and the diameter of the stipe is 1.0cm to 3.5cm. After the strain is inoculated, the demand for nutrients is much higher, especially the demand for lignin is much higher than that of ordinary edible fungi, resulting in its The mycelium growth cycle is long, and the fruiting time is long. Shortening the growth cycle of seafood mushrooms is one of the long-term research topics of seafood mushroom cultivation manufacturers. [0003] Compared with solid strains, liquid strains have the advantages of consistent bacterial age, neat fruiting, easy management, low...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/00A01G18/20
Inventor 范跃有张卓正魏志鹏林风范潇斌
Owner SICHUAN JUNLYU ECOLOGICAL AGRI TECH CO LTD
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