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Method for supercritical extraction of sea buckthorn oil

A supercritical and seabuckthorn oil technology, which is applied in the direction of fat oil/fat production and fat generation, can solve the problems of supercritical equipment blockage, poor fluidity of lipid substances, and no formation of free unsaturated fatty acids, so as to improve utilization efficiency and ensure The effect of using safety and improving quality

Inactive Publication Date: 2012-07-04
刘正芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The above method shows that seabuckthorn dried fruit or pomace is directly extracted, and seabuckthorn has not been processed by any biological method. Because there are a large amount of protein and lipid substances in seabuckthorn fruit, the protein and lipid substances are not utilized in the process of direct extraction of seabuckthorn oil. The conversion is not complete, and a large amount of free unsaturated fatty acids are not formed, resulting in less free unsaturated fatty acids and very few free amino acids in seabuckthorn oil
In addition, the fluidity of its lipids is worse than that of unsaturated fatty acids, and direct supercritical extraction is likely to cause blockage of supercritical equipment

Method used

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  • Method for supercritical extraction of sea buckthorn oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] After 10kg of fresh seabuckthorn fruit is mixed with an equal volume of water, minced and homogenized, place it in an incubator and add 250U protease and 450U lipase to the homogenate (protease and lipase can be purchased from Changsha Xiangzi Biotechnology Co., Ltd.) , keep warm at 40°C for 10 h, put the enzymolysis solution in a 30L barrel, put it into the extraction kettle, install the pressure ring and sealing ring, tighten the plug, then turn on the freezer, put the extraction kettle, Adjust the temperature controllers of separation kettle Ⅰ and separation kettle Ⅱ to the required temperature respectively; when the temperature of the refrigerator drops to about 0°C, the extraction kettle and separation kettle Ⅱ respectively reach 45°C, and the temperature of separation kettle Ⅰ reaches 50°C, Open the cylinder, open valve 2 (CO 2 Liquefied in the cold cycle), then turn on the high-pressure pump to pressurize, when the extraction pressure of the extraction kettle rea...

Embodiment 2

[0033] Mix 100kg of fresh seabuckthorn fruit with an equal volume of water, mince and homogenize it, put it in an incubator, add 3000U protease and 5000U lipase to the homogenate, keep it warm at 40°C for 10 hours, and put its enzymolysis solution in 300L Put it into the extraction kettle, install the pressure ring and sealing ring, tighten the upper plug, then turn on the refrigerator, and adjust the temperature controllers of the extraction kettle, separation kettle Ⅰ, and separation kettle Ⅱ to the required settings. After the temperature of the refrigerator drops to about 0°C, the temperature of the extraction kettle and the separation kettle II reaches 45°C, and the temperature of the separation kettle I reaches 50°C, open the gas cylinder and open the valve 2 (CO 2 Liquefied in the cold cycle), then turn on the high-pressure pump to pressurize, when the extraction pressure of the extraction kettle reaches 35MPa, adjust the shut-off valve to balance the pressure of the ext...

Embodiment 3

[0035] Mix 200kg of fresh seabuckthorn fruit with an equal volume of water, mince and homogenize it, put it in an incubator, add 5500U protease and 9000U lipase to the homogenate, keep it warm at 40°C for 10 hours, and put its enzymolysis solution in 300L Put it into the extraction kettle, install the pressure ring and sealing ring, tighten the upper plug, then turn on the refrigerator, and adjust the temperature controllers of the extraction kettle, separation kettle Ⅰ, and separation kettle Ⅱ to the required settings. After the temperature of the refrigerator drops to about 0°C, the temperature of the extraction kettle and the separation kettle II reaches 45°C, and the temperature of the separation kettle I reaches 50°C, open the gas cylinder and open the valve 2 (CO 2 Liquefied in the cold cycle), then turn on the high-pressure pump to pressurize, when the extraction pressure of the extraction kettle reaches 35MPa, adjust the shut-off valve to balance the pressure of the ext...

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Abstract

The invention mainly relates to a method for supercritical extraction of sea buckthorn oil, which includes: using fresh sea buckthorn fruits as raw materials, smashing, performing enzymolysis, and supercritically extracting to obtain sea buckthorn seed oil. The method makes full use of proteins in the sea buckthorn fruits in the whole extraction process, enables the sea buckthorn oil to contain a large amount of free essential amino acid and more free unsaturated fatty acid, greatly improves quality of the sea buckthorn oil. The method for supercritical extraction of the sea buckthorn oil avoids the problem of blocking of a device after enzymolysis lipid substances are adopted, guarantees use safety of the device, and improves utilization ratio of the device.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a method for supercritically extracting seabuckthorn oil. Background technique [0002] Seabuckthorn ( Hippophae hamoides Linn. ) is a plant of the genus Seabuckthorn in the family Echinacea, a deciduous shrub or small tree, also known as Suanliu, Suanliu, Qicharigacha (Mongolian name), Dapu (Tibetan name), and Jihan (Uyghur name). It is mainly distributed in more than ten provinces and regions in Northwest my country, North China, Northeast China and Southwest China, with about 1 million hm2 in the country. 2 . It is a commonly used drug in ancient Tibetan medicine and Mongolian medicine in my country. It was officially included in the Chinese Pharmacopoeia in 1977. Seabuckthorn is a medicinal plant containing more than 190 active substances such as vitamins, trace elements, amino acids and flavonoids. In recent years, research reports on chemistry, biol...

Claims

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Application Information

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IPC IPC(8): C11B1/10
Inventor 刘喜荣刘正芳王腊霜
Owner 刘正芳
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