The invention discloses cell culture equipment, and belongs to the technical field of biomedical cell culture. The cell culture equipment mainly comprises a sample injection chamber, a culture chamberand a motor chamber, and specifically comprises a culture dish conveying device, a culture dish-cover separation and closing device, a liquid changing device, an air filtering system, a disinfectingheating device, an automatic control system and a culture dish. According to the present invention, with the cell culture equipment, the isolation between the culture chamber and the outside world isachieved, the culture condition of the cells in the culture dish can be real-timely observed or shot and recorded, the automatic replacement of the culture liquid is achieved, and the gas in the culture dish and the gas in the culture chamber can be freely and smoothly exchanged while the water in the culture dish cannot be removed from the culture dish by evaporation. In the prior art, the pollution in the incubator is easily caused during the taking and placing of the culture dish, the manual adding or replacing of the culture liquid is cumbersome in the operation and easily causes the liquid changing error or the culture liquid pollution, and the growth condition of the cells in the culture dish is inconveniently observed. With the cell culture equipment of the present invention, the problems in the prior art can be solved.