Preparation method and application of providencia stuartii enzyme agent

A Providencia stutzeri enzyme agent and Providencia bacteria technology are applied in the field of biotechnology to degrade pesticide residues, and can solve the problems of no sclerotia-free biodegradation, etc., and achieve environmental friendliness, good quick-acting performance, and low cost. cheap effect

Inactive Publication Date: 2019-05-31
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To date, no research has been done on the biodegradation of sclerotinia in agricultural products

Method used

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  • Preparation method and application of providencia stuartii enzyme agent

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Embodiment 1

[0017] 1. Enzyme preparation:

[0018] 1) Bacterial suspension preparation: Add 2 mL of sterile phosphate buffer under aseptic conditions and shake in parallel in the culture dish cultured for 48 hours, and gently scrape off the colonies on the surface of the medium with an inoculation loop to form a crude bacterial suspension. liquid, and transferred it to a liquid fermentation medium (beef extract 8g / L, yeast extract 14g / L, NaCl 5g / L) and cultured for 48h at pH 7-8, 35°C, 150-200rpm. Immediately centrifuge the fermented broth taken out at 10,000 r / min at 4°C for 10 min to collect the wet cells and wash them three times with sterile phosphate buffered saline. Then mix the sterile phosphate buffer solution with the wet cells, and control the number of cells to be ≥6×10 10 cfu / mL. The prepared bacterial suspension was stored at 4°C for future use.

[0019] 2) Extraction and preparation of degrading enzyme agent: the bacteria suspension prepared in step 1) was subjected to ul...

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Abstract

The invention discloses a preparation method and application of a providencia stuartii enzyme agent. According to the preparation method, with providencia stuartii CCTCC M 2017738 as a strain, the providencia stuartii enzyme agent is prepared through expanding propagation of a bacterial suspension and ultrasonic crushing extraction of a degrading enzyme agent. The degrading enzyme agent can be applied to efficient degradation of dimethachlon, vinclozolin and iprodione in plants such as oilseed rape, Chinese cabbage, purple pakchoi, lettuce, fragrant-flowered garlic, cucumbers, tomatoes, peppers, potatoes and tobaccos, soil and water. The providencia stuartii enzyme agent has the advantages of being good in quick-acting performance, environmentally friendly, capable of achieving an obviouseffect, low in cost, easy to popularize and the like.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to a preparation method and application of a Providencia stutzeri enzyme agent. Background technique [0002] Pesticides play an important role in the integrated management of agricultural pests, but pesticide residues and their metabolites have become serious pollutants, posing threats to human health and ecosystems. Dimetachlone, also known as Dimetachlone, has molecular formula C 10 h 7 Cl 2 NO 2 , its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl)succinimide. In my country, it is widely used in the prevention and control of fungal diseases such as sclerotinia, sheath blight, and red spot on rapeseed, Chinese cabbage, laver moss, lettuce, leek, cucumber, tomato, pepper, potato, tobacco and other crops. Despite its promising bactericidal activity, sclerotinia is currently banned in the US and Europe due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N9/00A01N25/32C09K17/14C02F3/34C12R1/01C02F101/38
Inventor 吴小毛张承李荣玉龙友华李姣红王秋萍黄文源代园凤
Owner GUIZHOU UNIV
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