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Oil-containing sewage degradation compound bacterium and preparation method thereof

A technology of compound bacteria and sewage, applied in the fields of biochemical equipment and methods, chemical instruments and methods, biological water/sewage treatment, etc. Effect

Active Publication Date: 2019-04-23
BC P INC CHINA NAT PETROLEUM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at overcoming the problem that the existing biological bacterial agent in the background technology is effective in treating oily sewage mainly for a single index and has high requirements on the environment, and provides an oily sewage degrading composite bacterium and its preparation method

Method used

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  • Oil-containing sewage degradation compound bacterium and preparation method thereof
  • Oil-containing sewage degradation compound bacterium and preparation method thereof
  • Oil-containing sewage degradation compound bacterium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Taking the sewage treatment experimental base of Daqing Water Affairs Company as an example, to evaluate the effect of treating oily sewage, the influent water is taken from the Changyuan Biochemical Station of the No. 1 Oil Production Plant, and the pH value of the influent water is 8.5. After research, the pilot process was determined to be catalytic air flotation-reaction precipitation-contact oxidation-sand filtration. The processing capacity of the whole set of equipment is 1m 3 / h. Activate the strains in LB medium at 37°C for 48 hours, count the total number of colonies, dilute each strain to the same order of magnitude with sterile saline, and prepare bacterial suspensions with 1% of the amount of treated sewage Proportional dosing, in the traditional contact oxidation tank, place fillers, and add bacteria and nutrients in the early stage. Carry out pre-hanging film-hanging film-acclimation, and measure the water in and out of this section. Through a period o...

Embodiment 2

[0024] The 106-station pilot test of the No. 1 Oil Production Plant was used to evaluate the effect of treating oily sewage, and the pH value of the influent water was 9.5. The pilot process is raw water—dissolved air flotation—biological contact oxidation—precipitation—two-stage filtration. The processing capacity is 2m 3 / h, the raw water is incoming water from 106 stations; only for biological bacteria agents, in the contact oxidation tank, place fillers, add bacteria agents in the early stage, the activation and addition of bacteria agents are the same as in Example 1, and carry out pre-film-hang-film-acclimation , to measure the water in and out of this section, and through a period of data collection, it can be seen that the effect of the microbial agent is relatively stable. On the 31st-90th day of the pilot test, the treatment capacity of the flowing oily sewage is 2m 3 / h. At this stage, no additional substances were added. It can be seen that the trend of change i...

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Abstract

The invention relates to an oil-containing sewage degradation compound bacterium. The compound bacterium mainly solves the problems that an existing biological bacterial agent only has effects on oneindex of oil-containing sewage and is relatively high in environmental requirement. The oil-containing sewage degradation compound bacterium comprises providencia rettferi, morganella morganii and pseudomonas aeruginosa in the volume ratio of 1:1:1. A preparation method includes the steps that the three strains are cultured in an LB culture medium respectively for 48 h at 37 DEG C, a bacteria solution of each strain and glycerin are mixed, placed in an aseptic centrifuge tube and stored at a low temperature, the mixtures are placed to be at room temperature before use and are activated in theLB culture medium, the strains are diluted to the same magnitude order by using sterile normal saline according to the total number of bacterial colonies, prepared bacterium suspensions are fully mixed in proportion, and the mixed bacterial agent is obtained. The oil-containing sewage degradation compound bacterium can be used for simultaneously degrading oil and COD and can adapt to more severe treatment environments.

Description

technical field [0001] The invention relates to the technical field of biological preparations for oilfield sewage treatment, in particular to a compound bacterium for degrading oily sewage and a preparation method thereof. Background technique [0002] At present, biological treatment methods are widely used in domestic sewage treatment. There are many studies on oily sewage treatment, but the effect is not much good. The treatment of oily sewage is still in the research and development stage in China. At present, the domestic use of biological bacteria to treat oily sewage is mainly for A single indicator is effective, for example, it can be used alone to degrade oil, degrade COD, reduce viscosity, and reduce suspended solids. At present, there are many products used to degrade oil, but there are few products used to degrade COD. At the same time, there are already biological agents. The treatment of oily sewage has higher environmental requirements. The biological bacteri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20
Inventor 季远玲温雪邢楠楠葛蕊
Owner BC P INC CHINA NAT PETROLEUM CORP
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