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Composite micro-ecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome of Pacific white shrimps and application of composite micro-ecological preparation

A technology of compound microecology and hepatopancreas, applied in the direction of microorganisms, microorganism-based methods, medical preparations containing active ingredients, etc., to achieve the effect of reducing high mortality, significant application effect, and strong inhibition of Vibrio parahaemolyticus

Active Publication Date: 2019-01-08
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Abstract
  • Description
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a compound microecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome of Penaeus vannamei and its application, which overcomes the fact that none of the existing products is specifically aimed at acute hepatopancreatic necrosis syndrome of Penaeus vannamei Defects of probiotics

Method used

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  • Composite micro-ecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome of Pacific white shrimps and application of composite micro-ecological preparation
  • Composite micro-ecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome of Pacific white shrimps and application of composite micro-ecological preparation

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Embodiment 1

[0022] This embodiment provides a composite microecological preparation, including Providencia sp. and Enterobacter sp.; wherein, the Providencia preservation number is CGMCC No.15415, and the Enterobacter preservation The number is CGMCC No.15416. The two kinds of bacteria are respectively prepared by liquid fermentation at a ratio of 1:1. The two bacteria were cultured separately in advance to a concentration of 1.0×10 8 CFU / mL and mix for later use. Among them, the medium formula is: peptone 5g, yeast extract 1g, ferric phosphate 0.01g, sodium acetate 3g, dipotassium hydrogen phosphate 1g, aged seawater 1000mL, pH value 7.6-7.8.

[0023] The method of using the compound probiotics is as follows:

[0024] Add the culture mixture of Providencia and Enterobacter to the Penaeus vannamei pellet feed, the mixing ratio is 1:50, feed the mixed feed to Penaeus vannamei, and observe the eating situation of the bait table. Every day from 8:00 to 9:00 in the morning, the animals we...

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Abstract

The invention relates to a composite micro-ecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome (AHPNS) of Pacific white shrimps and application of the composite micro-ecological preparation. The preparation comprises providencia vermicola and enteric bacilli. The composite micro-ecological preparation is applied together with feed. The composite micro-ecological preparation provided by the invention has a good effect in inhibiting Vibrio parahemolyticus, has a remarkable application effects, is one of significant ways for green breeding of the Pacificwhite shrimps and has good application prospects.

Description

technical field [0001] The invention belongs to the field of aquatic product disease prevention and control, and in particular relates to a compound microecological preparation for preventing and controlling acute hepatopancreatic necrosis syndrome of Penaeus vannamei and its application. Background technique [0002] Acute hepatopancreatic necrosis syndrome (AHPNS) of Penaeus vannamei is a disease characterized by sudden death of shrimp due to hepatopancreas necrosis, and is one of the most serious diseases affecting shrimp farming in recent years. The early stage of the disease mainly occurs in the juvenile stage of prawns, collectively referred to as early mortality syndrome (Early mortality syndrome, EMS). It has been found that some Vibrio carrying specific pathogenic genes are the pathogens causing the disease, mainly Vibrio parahaemolyticus. Tran et al. isolated a strain of Vibrio harveyi from a shrimp pond with AHPNS disease in Vietnam, and the mortality rate of the...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/18C12N1/20C12R1/01
CPCA61K35/74A61P1/18C12N1/20A61K2300/00
Inventor 沈辉甄晓然万夕和蒋葛乔毅王李宝史文军黎慧范贤平
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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