High-efficiency denitrification compound microbial inoculum, and preparation and application thereof

A compound bacterial agent and denitrification technology, which is applied in the direction of bacteria, water treatment parameter control, biochemical equipment and methods, etc., can solve the problems of low denitrification capacity, poor adaptability of saline wastewater, long domestication period, etc., and achieve denitrification Improved effect, good denitrification ability, strong targeted effect

Inactive Publication Date: 2019-11-05
JIANGSU LASON CHEM ENVIRONMENTAL PROTECTION
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AI Technical Summary

Problems solved by technology

[0004] In recent years, microbial agents have been used in water environment improvement, but there are still many problems, such as some microorganisms cannot produce enzymes, the effect is single, and most of them stay in the laboratory stage, it is difficult to be widely used
In the sewage ecosystem, a single microorganism has poor stability, low denitrification ability, poor adaptability to saline wastewater, and long domestication period

Method used

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  • High-efficiency denitrification compound microbial inoculum, and preparation and application thereof
  • High-efficiency denitrification compound microbial inoculum, and preparation and application thereof

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preparation example Construction

[0035] The invention also discloses a preparation method of a high-efficiency denitrification compound bacterial agent, and the steps are as follows:

[0036] Step 1: Solid plate culture: Nitrobacter, Alcalibacterium aquagenicum, Paracoccus nitricophila, Bacillus subtilis, Bacillus cereus, and Providencia were transferred to solid medium, and cultured at 20-40°C Incubate in the box for 24-36h to activate the strain;

[0037] Step 2: Seed liquid culture: inoculate the strains activated in step 1 into the liquid medium respectively, and conduct shaking culture at 20-40°C and 100-200rpm for 24-36h, until the strains are in the logarithmic growth phase;

[0038] Step 3: Expanded culture: Fermenting the seed liquor obtained in Step 2 to obtain a denitrifying compound bacterial agent.

[0039] The high-efficiency denitrification compound bacterial agent prepared by the invention is the application in the nitrification biological filter, and the specific steps are as follows:

[00...

Embodiment 1

[0053] The sewage was taken from a pharmaceutical company in Hebei, the COD was about 800mg / L, and the ammonia nitrogen was about 240mg / L. The combined carrier is used as the filler. According to the application method of the composite bacterial agent of the present invention in the sewage treatment of engineering implementation, carry out film hanging, biological aerobic aeration, HRT is 12h, regular sampling is used to measure ammonia nitrogen concentration with Nessler reagent spectrophotometry, and blank aeration is set In contrast, the degradation rate of ammonia nitrogen using the compound bacterial agent of the present invention reaches 97%. The specific results are shown as follows figure 1 shown.

Embodiment 2

[0055] Select a certain salty wastewater with COD of 280mg / L, ammonia nitrogen of about 200mg / L, and 2% sulfate. According to the application method of the composite bacterial agent of the present invention in the sewage treatment of engineering implementation, the film is formed, and the biological aerobic exposure is carried out. gas, HRT is 24h, regular sampling is used to measure the ammonia nitrogen concentration with Nessler reagent spectrophotometry, and a blank aeration control is set, the degradation rate of ammonia nitrogen using the compound bacterial agent of the present invention reaches 97%, and the specific results are shown in the figure. figure 2 shown.

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Abstract

The invention discloses a high-efficiency denitrification compound microbial inoculum, and preparation and an application thereof, and belongs to the field of biotechnology. The microbial inoculum forwastewater treatment mainly includes six strains including nitrobacter sp, nitrosomonas sp, alcaligenes aquatilis, paracoccus nitrovorans, bacillus cereus, providencia vermicola and the like. A culture method includes enrichment, purification and enlarged culture of bacteria; the used microbial inoculum has the advantages of high denitrification efficiency, short domestication time, salt tolerance and the like, and is suitable for large-scale production. The obtained microbial inoculum can effectively treat high ammonia-nitrogen wastewater. The microbial preparation still has good denitrification ability under the condition of 2% total salt, solves the limitation of high-salt environment on a traditional biological denitrification method, and has broad application prospect and good socialbenefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a high-efficiency denitrification compound bacterial agent, preparation and application thereof. Background technique [0002] With the rapid development of fermentation, chemical and pharmaceutical industries, the treatment of high-concentration ammonia nitrogen wastewater has received more and more attention. Biological denitrification of wastewater in my country is currently a hot spot of concern and research in the field of water treatment. At present, the treatment of high ammonia nitrogen wastewater mainly includes physicochemical, biochemical and natural ecological treatment technologies. Biological denitrification technology has become one of the best denitrification methods due to its low investment and operating costs, simple operation, no secondary pollution, and high denitrification efficiency. thorough. [0003] At present, there are two types of biological denitrifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C12R1/05C12R1/125C12R1/08
CPCC12N1/20C02F3/34C02F3/02C02F2209/22Y02W10/10
Inventor 邵汝英蔡凯李琳蒋伟群王镔赵振华高峰王帅
Owner JIANGSU LASON CHEM ENVIRONMENTAL PROTECTION
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