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Application of Providencia in production of hydroxyethyl piperazine ethanesulfonic acid

A technology of hydroxyethylpiperazineethanesulfonic acid and Providencia bacteria, applied in the field of biosynthesis, can solve problems affecting product quality and yield, and difficulties in the separation process

Pending Publication Date: 2021-08-10
永州恒飞生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the usual process for producing hydroxyethylpiperazineethanesulfonic acid is to use hydroxyethylpiperazine and sodium vinylsulfonate to react to generate sodium hydroxyethylpiperazineethanesulfonate, and then use hydrochloric acid or sulfuric acid to acidify to obtain hydroxyethylpiperazine Piperazineethanesulfonic acid, a large amount of sodium chloride solid waste is produced in the process, and hydroxyethylpiperazineethanesulfonic acid has a large amount of sodium remaining, which is easy to undergo condensation reaction by itself and form high polymer, which makes the separation process difficult in the later stage , affecting product quality and yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 7 g of sodium chloroethanesulfonate to 100 mL of the solution in which 0.5 g of hydroxyethylpiperazineethanesulfonic acid was added, and slowly add dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% until the pH value rises to 6. The temperature of the transformation flask was controlled between 35 and 38°C, and 0.1 g of Providencia bacteria was inserted into it, and slowly added dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% according to the pH value, and the pH value was kept constant. After 36 hours, the dropwise addition of hydroxyethylpiperazine was completed, and the clear liquid obtained by filtering with a microporous filter after sterilization at 80°C was taken out, and analyzed by liquid chromatography, the result was hydroxyethylpiperazineethanesulfonic acid and hydroxyethylpiperazine The content ratio of piperazine is 99.45:0.55.

Embodiment 2

[0028] Add 10 g of sodium chloroethanesulfonate to 100 mL of a solution in which 1 g of hydroxyethylpiperazineethanesulfonic acid was added, slowly add dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% until the pH value rises to 6, and the conversion The temperature of the bottle was controlled between 35 and 38°C, and 0.1g of Providencia bacteria was inserted into it, and slowly added dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% according to the pH value, and the pH value was controlled to remain unchanged. After 36 hours, the dropwise addition of hydroxyethylpiperazine was completed, and the clear liquid obtained by filtering with a microporous filter after sterilization at 80°C was taken out, and analyzed by liquid chromatography, the result was hydroxyethylpiperazineethanesulfonic acid and hydroxyethylpiperazine The content ratio of oxazine is 99.50:0.50.

Embodiment 3

[0030] Add 1 g of sodium chloroethanesulfonate to 100 mL of the solution in which 1 g of hydroxyethylpiperazineethanesulfonic acid was added, slowly add dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% until the pH value rises to 6, and the conversion The temperature of the bottle was controlled between 35 and 38°C, and 0.1g of Providencia bacteria was inserted into it, and slowly added dropwise an aqueous solution of hydroxyethylpiperazine with a mass concentration of 50% according to the pH value, and the pH value was controlled to remain unchanged. After 36 hours, the dropwise addition of hydroxyethylpiperazine was completed, and the clear liquid obtained by filtering with a microporous filter after sterilization at 80°C was taken out, and analyzed by liquid chromatography, the result was hydroxyethylpiperazineethanesulfonic acid and hydroxyethylpiperazine The content ratio of oxazine is 99.32:0.68.

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Abstract

The invention provides application of Providencia in production of hydroxyethyl piperazine ethanesulfonic acid, and belongs to the technical field of biosynthesis. According to the method disclosed by the invention, the isethionesulfonic acid is produced by biotransformation of isethionesulfonic acid from isethionesulfonic acid and sodium chloroethanesulfonate by adopting Providencia, no organic reagent is used in the transformation process, normal-pressure operation can be carried out, the reaction condition is mild, the purity of isethionesulfonic acid obtained by transformation is high, and the yield of a transformation product subjected to purification treatment and HPLC detection reaches 99% or above, and the purity is greater than or equal to 99% (HPLC).

Description

technical field [0001] The invention relates to the technical field of biosynthesis, in particular to the application of Providencia bacteria in the production of hydroxyethylpiperazineethanesulfonic acid. Background technique [0002] Hydroxyethylpiperazineethanesulfonic acid is a widely used biological buffer, which can control the pH value of the system to be constant for a long time, and will not complex with metal ions. Hydroxyethylpiperazineethanesulfonic acid is widely used in the field of cosmetics, and can be used to prepare whitening products, repair products, anti-aging products and fruit acid products; Hydroxyethylpiperazineethanesulfonic acid is also widely used in the biomedical industry and biological Vaccine cultivation industry; in other industries, hydroxyethylpiperazineethanesulfonic acid can also be used to prepare cell culture fluid, organ preservation fluid, virus preservation fluid, low temperature (frozen) sperm preservation dilution and buffer soluti...

Claims

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Application Information

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IPC IPC(8): C12P17/12C12N1/20C12R1/01
CPCC12P17/12C12N1/20
Inventor 伍雄飞刘明明伍雄姿唐斌伍晓玲
Owner 永州恒飞生物医药有限公司
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