Small brevibacterium strain capable of carrying out biological denitrification under high-salt condition and application of small brevibacterium strain to wastewater treatment

A technology of brevibacteria and high-salt wastewater, which is applied in the field of biological denitrification, can solve the problems of unsatisfactory biological denitrification effect, achieve the effects of saving infrastructure and operating costs, broad application prospects, and simplifying the process flow

Active Publication Date: 2012-10-03
北京大学科技开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, according to the existing research, the effect of biolo

Method used

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  • Small brevibacterium strain capable of carrying out biological denitrification under high-salt condition and application of small brevibacterium strain to wastewater treatment
  • Small brevibacterium strain capable of carrying out biological denitrification under high-salt condition and application of small brevibacterium strain to wastewater treatment
  • Small brevibacterium strain capable of carrying out biological denitrification under high-salt condition and application of small brevibacterium strain to wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Example 1. Screening of Brachybacterium strains with high-salt heterotrophic nitrification-aerobic denitrification performance

[0038] Specific steps are as follows:

[0039] 1) Put 1mL of brine sample (collected from the sun-dried salt pool of Tianjin Hangu Salt Field) into 100mL of liquid medium (each L contains yeast extract: 5g, tryptone: 10g, NaCl: 3%-10%, pH: 7.0-7.5 , 121°C 20min steam sterilization) in a 250mL Erlenmeyer flask, 30°C 150r / min shaker culture for 5 days to enrich the bacteria;

[0040] 2) Dilute the enriched brine sample to 10 by the doubling dilution method -1 、10 -2 , ... 10 -6 For the bacterial suspension of each gradient, take the dilution as 10 -4 、10 -5 、10 -6 Each 0.1ml of the bacterial suspension was evenly spread on the pre-prepared high-salt solid medium (each L containing yeast extract: 5g, tryptone: 10g, NaCl: 3%-10%, agar: 16-18g, pH: 7.0-7.5, steam sterilization at 121°C for 20 minutes) on a 10ml solid plate;

[0041] 3) Place...

Embodiment 2

[0046] Embodiment 2. Bacterial strain is the denitrification experiment of 5% in salinity

[0047] Using glucose as the carbon source and ammonia nitrogen as the nitrogen source, the removal ability of the Brachybacterium strain described in Example 1 to ammonia nitrogen was determined. The specific implementation steps are as follows:

[0048] Inoculate the Brachybacterium strain in 100ml of inorganic salt medium with a salinity (calculated as NaCl) of 5% (containing 0.94g glucose and 0.153g NH per liter 4 Cl, 0.035g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0~7.5), cultured in a shaker at 30°C at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. A small amount of reaction solution was taken at 6h, 12h, 18h, 24h, 36h and 48h, a part of which was directly used to measure the optical density of the bacteria, and the rest was centrifuged at 8000rpm for...

Embodiment 3

[0050] Example 3. Denitrification experiments of bacterial strains under different salinity conditions

[0051] Using glucose as the carbon source and ammonia nitrogen as the nitrogen source, the Brachybacterium strains described in the examples were tested for their ability to remove ammonia nitrogen at different salinities. The specific implementation steps are as follows:

[0052] Inoculate Brachybacterium strains in 100ml of inorganic salt medium with salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13%, shake at 30°C at 150rpm pre-cultured in the bed. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial solution and insert it into 90ml of fresh inorganic salts with a salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13% for culture Culture medium was shaken at 30°C and 150 rpm in a shaker. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same c...

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Abstract

The invention discloses a small brevibacterium strain capable of carrying out biological denitrification under the high-salt condition and application of the small brevibacterium strain. The small brevibacterium strain provided by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on March 29th, 2012, with the preservation number of CGMCC No.5947. At the salinity (counted by NaCl) is 3-10 percent, the strain is added into nitrogen-containing wastewater with dissolved oxygen of 2-6mg/L for biological denitrification. The strain has strong resistant ability for high salinity and heterotrophic nitrification and aerobic denitrification capabilities and can better grow under the high-salinity condition. Under the high-salinity and aerobic conditions, the strain can be used for synchronous nitrification and denitrification and efficiently and thoroughly removing total nitrogen in sewage, and thus the major problem that biological denitrification is difficult to carry out under the high-salinity condition is effectively solved and a favorable application prospect is obtained.

Description

technical field [0001] The invention relates to the field of biological denitrification, in particular to a Brachybacterium strain used for high-salt biological denitrification and its application. Background technique [0002] With the progress of the society and the development of the economy, the eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of blue-green algae red tides occurs frequently, which has caused huge damage to the safety of water bodies and human health. Excess nutrients such as nitrogen and phosphorus are the root cause of water eutrophication. It is generally believed that eutrophication may occur when the concentration of N in the water body is >0.2mg / l and the concentration of P is >0.02mg / l. Domestic sewage is the largest discharge source of nutrients such as nitrogen and phosphorus; for this reason, my country has been committed to developing a variety of domestic sewage denitrific...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 倪晋仁邓若男
Owner 北京大学科技开发有限公司
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