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High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment

A high-salt wastewater and halovibrio technology, applied in the field of biological denitrification, can solve problems such as the unsatisfactory biological denitrification effect, and achieve the effects of saving infrastructure and operating costs, good social benefits, and simplifying the process flow

Active Publication Date: 2012-10-24
中节能铁汉盖雅(北京)环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the existing research, the effect of biological nitrogen removal in high-salt wastewater is not very ideal.

Method used

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  • High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment
  • High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment
  • High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Example 1. Screening of Salinivibrio strains with high-salt heterotrophic nitrification-aerobic denitrification performance

[0039] Specific steps are as follows:

[0040] 1) Put 1mL of brine sample (collected from the sun-dried salt pool of Tianjin Hangu Salt Field) into 100mL of liquid medium (each L contains yeast extract: 5g, tryptone: 10g, NaCl: 3%-10%, pH: 7.0-7.5 , 121°C 20min steam sterilization) in a 250mL Erlenmeyer flask, 30°C 150r / min shaker culture for 5 days to enrich the bacteria;

[0041] 2) Dilute the enriched brine sample to 10 by the doubling dilution method -1 、10 -2 , ... 10 -6 For the bacterial suspension of each gradient, take the dilution as 10 -4 、10 -5 、10 -6 Each 0.1ml of the bacterial suspension was evenly spread on the pre-prepared high-salt solid medium (each L containing yeast extract: 5g, tryptone: 10g, NaCl: 3%-10%, agar: 16-18g, pH: 7.0-7.5, steam sterilization at 121°C for 20 minutes) on a 10ml solid plate;

[0042] 3) Place t...

Embodiment 2

[0047] Embodiment 2. Bacterial strain is the denitrification experiment of 3% at salinity

[0048] Using glucose as the carbon source and ammonia nitrogen as the nitrogen source, the removal ability of the Salinivibrio strain described in Example 1 to ammonia nitrogen was determined. The specific implementation steps are as follows:

[0049] Inoculate Salinivibrio strains in 100ml of inorganic salt medium with a salinity (calculated as NaCl) of 3% (containing 0.94g glucose, 0.153g NH per liter 4 Cl, 0.035g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, 0.006g FeSO 4 ·7H 2 O, pH 7.0~7.5), cultured in a shaker at 30°C at 150 rpm. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. A small amount of reaction solution was taken at 6h, 12h, 18h, 24h, 36h and 48h, a part of which was directly used to measure the optical density of the bacteria, and the rest was centrifuged at 8000rpm for 10min, and ...

Embodiment 3

[0051] Example 3. Denitrification experiments of bacterial strains under different salinity conditions

[0052] Using glucose as the carbon source and ammonia nitrogen as the nitrogen source, the removal ability of the Salinivibrio strains described in the examples to ammonia nitrogen at different salinities was determined. The specific implementation steps are as follows:

[0053] Salinivibrio strains were inoculated in 100ml of inorganic salt medium with salinity (calculated as NaCl) of 3%, 5%, 8%, and 10%, respectively, and pre-cultured in a shaker at 30°C and 150rpm. When the strain grows to the late logarithmic growth phase, take 10ml of the bacterial solution and insert it into 90ml of fresh inorganic salts with a salinity (calculated as NaCl) of 1%, 3%, 5%, 8%, 10% and 13% for culture Culture medium was shaken at 30°C and 150 rpm in a shaker. The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the ...

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Abstract

The invention discloses a salinivibrio strain which can carry out biological denitrification under high-saline conditions, and an application thereof. The salinivibrio strain provided by the invention is collected in China General Microbiological Culture Collection Center (CGMCC) on 29th March, 2012 with a collection number of CGMCC No.5946. With a salinity (calculated according to NaCl) below 3-10%, the strain is placed in nitrogen-containing wastewater with dissolved oxygen of 2-6mg / L, such that biological denitrification can be carried out. The strain has high tolerance to high salinity, and grows well in a high-saline condition. The strain has both heterotrophic nitrification and aerobic denitrification capacities. Under a high-saline aerobic condition, the strain can carry out synchronous nitrification and denitrification, such that total nitrogen in sewage can be completely removed with high efficiency. Therefore, a major problem of biological denitrification under high-saline conditions is effectively solved, and a good application prospect is provided.

Description

technical field [0001] The invention relates to the field of biological denitrification, in particular to a salt vibrio strain (Salinivibrio) used for high-salt biological denitrification and an application thereof. Background technique [0002] With the progress of the society and the development of the economy, the eutrophication pollution of various water bodies in our country is becoming more and more serious, and the problem of blue-green algae red tides occurs frequently, which has caused huge damage to the safety of water bodies and human health. Excess nutrients such as nitrogen and phosphorus are the root cause of water eutrophication. It is generally believed that eutrophication may occur when the concentration of N in the water body is >0.2mg / l and the concentration of P is >0.02mg / l. Domestic sewage is the largest discharge source of nutrients such as nitrogen and phosphorus; for this reason, my country has been committed to developing a variety of domestic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCY02W10/10
Inventor 邓若男陈倩付东康
Owner 中节能铁汉盖雅(北京)环境科技有限公司
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