Achromobacter xylosoxidans strain for biological denitrificaion and application thereof

A colorless bacillus and biological denitrification technology, applied in the field of biological denitrification, can solve problems such as lengthy process, achieve broad application prospects, save equipment and investment costs, and simplify the process flow

Inactive Publication Date: 2009-10-21
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological denitrification process developed according to the traditional biological denitrification theory usually carries out nitrification and denitrification in the aerobic zone and anoxic zone respectively, forming a graded nitrification and denitrification process, so the nitrification pool and the denitrification pool must be built separately, so that There are many deficiencies in the graded nitrification and denitrification process: (1) lengthy process
However, there are few reports on bacteria capable of both heterotrophic nitrification and aerobic denitrification, and the practical application of such special bacteria in wastewater denitrification treatment is still blank.

Method used

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  • Achromobacter xylosoxidans strain for biological denitrificaion and application thereof
  • Achromobacter xylosoxidans strain for biological denitrificaion and application thereof
  • Achromobacter xylosoxidans strain for biological denitrificaion and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Obtainment of strain GAD3 with heterotrophic nitrification-aerobic denitrification performance and biological denitrification experiment

[0038] 1. Isolation and purification method of bacterial strain GAD3 with heterotrophic nitrification-aerobic denitrification performance

[0039] The method has the following steps:

[0040] 1) Sludge samples were taken from the landfill leachate combined treatment system in Buji, Shenzhen;

[0041] 2) Inoculate into a 500mL Erlenmeyer flask containing 100mL enriched culture medium: each L contains 0.95g NaNO 3 , 0.7g peptone, 0.5g beef extract, 0.15g urea, 0.04g NaCl, 0.15g KH 2 PO 4 , 0.02g KCl, 0.03g MgSO 4 ·7H 2 O, 0.20g CaCl2 2H 2 O, pH 7.0-7.5;

[0042] 3) Wrap the mouth of the bottle with gauze, and culture it on a shaker at 30° C. and 140 rpm for 4 days;

[0043] 4) Take the bacterial solution in the shake flask as the strain source, take out 10mL and inoculate it into a fresh Erlenmeyer flask containing 1...

Embodiment 2

[0062] Embodiment 2. Biological denitrification experiment

[0063] The bacterial strain GAD3 prepared by the method provided in Example 1 and its bacterial suspension were subjected to biological denitrification experiments.

[0064] 1. Ammonia nitrogen (NH 4 + -N) as nitrogen source biological denitrification experiments

[0065] Get 5mL of the prepared bacterial strain GAD3 bacterium suspension, add in three culture bottles containing 95ml test medium (each L medium contains 1.52g NH 4 Cl, 1.0g KH 2 PO 4 , 0.06g FeSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 1.0g MgSO 4 ·7H 2 (0, 8.1 g sodium citrate, pH is 8.0), sealed with 9 layers of gauze, and incubated at 15° C. in a shaker at 160 rpm (radius of rotation: 15 mm). The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. The reaction solution was taken the next day, and centrifuged at 4000rpm for 10min, and the supernatant was taken t...

Embodiment 3

[0071] Embodiment 3. Biological denitrification condition experiment

[0072] The bacterial strain GAD3 prepared by the method provided in Example 1 and its bacterial suspension were subjected to biological denitrification condition experiments.

[0073] 1. Ammonia nitrogen (NH 4 + -N) as nitrogen source biological denitrification condition experiment

[0074] Get 5mL prepared bacterial strain GAD3 bacterium suspension, add in 9 culture flasks containing 95ml test medium (each L medium contains 0.57g NH 4 Cl, 1.0g KH 2 PO 4 , 0.06g FeSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 1.0g MgSO 4 ·7H 2 (2, 7.72g anhydrous sodium acetate), the initial pH is respectively 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0. Seal it with 9 layers of gauze, and cultivate it in a shaker at 40° C. and 160 rpm (rotation radius: 15 mm). The culture medium that was not inoculated with the bacterial suspension was used as a blank control for experiments under the same conditions. After culturing for 24 hours, ...

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Abstract

The invention discloses an achromobacter xylosoxidans strain for biological denitrificaion and application thereof. The achromobacter xylosoxidans strain GAD3 is preserved in China General Microbiological Culture Collection Center (CGMCC for short) with preservation number CGMCC No. 2964 in March 19th, 2009. The strain is added to nitrogenous effluent with 2 to 6mg / L of dissolved oxygen to realize biological denitrificaion. The strain not only has the capability of heterotrophic nitrification, but also has the capability of aerobic denitrification. In the process of treating the nitrogenous effluent, only one aerobic stage is needed, the denitrification efficiency is high, and the operation is convenient and quick; and compared with the conventional biological denitrification process, the application has huge economic benefits.

Description

technical field [0001] The invention relates to the field of biological denitrification, in particular to a strain of Achromobacter xyloseoxidans used for biological denitrification and application thereof. Background technique [0002] The main forms of nitrogen in wastewater are molecular nitrogen, organic nitrogen, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, and some nitrogen present in hydrogen sulfide and cyanide. Due to the toxic effect of ammonia nitrogen, the carcinogenic effect of nitrate, and the acidification and eutrophication of nitrogen compounds on ecosystems, rivers, lakes, and offshore oceans, how to economically and efficiently remove nitrogen in water has become a water pollution treatment technology. Research priorities and hotspots in the field of control. [0003] Among the various denitrification methods that have been applied so far, biological denitrification is still the main means of wastewater denitrification treatment. The traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/30C02F3/34C12R1/025C02F101/16
Inventor 倪晋仁陈倩
Owner PEKING UNIV
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