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Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof

An aerobic denitrification and halomonas technology, which is applied in the field of microorganisms to achieve the effects of shortening the startup time, increasing the growth rate, and having a high extreme value.

Inactive Publication Date: 2014-05-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a Halomonas campisalis strain Halomonascampisalis.BMEN3 which can efficiently carry out heterotrophic nitrification and aerobic denitrification at the same time in view of the problems in the prior art on the treatment of saline and nitrogenous wastewater and flue gas , the strain can simultaneously carry out nitrification and denitrification under aerobic conditions and high salinity environment, and finally convert NH 4 + , NO 3 - , NO 2 - Environmentally harmful substances are converted into harmless nitrogen

Method used

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  • Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof
  • Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof
  • Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Screening of Heterotrophic Nitrification and Aerobic Denitrification Strains

[0057] 1. Divide 10mL of the mud sample taken from Ordos Saline-Alkali Lake (1150m above sea level, 109.73° east longitude, 39.57° north latitude) in three parts into 250mL triangular flasks, which contained 100mL screening medium (formulation g / L: C 4 h 4 Na 2 o 4 ·6H 2 O 4.73, NaNO 3 0.85, NaCl 60, yeast extract 1, NH 4 SO 4 0.19, KH 2 PO 4 1.36, 5mL trace elements (g / L:Na 2 EDTA 63.7; MnCl 2 4H 2 05·06; FeSO 4 ·7H 2 O 5.022; CaCl 2 5.5; Na 2 MoO 4 4H 2 O 1.1; CuSO 4 ·5H 2 O 1.57; CoCl 2 ·6H 2 O 1.61; PH=7.00), adjust the pH to 7.5 with NaOH, seal with parafilm and sterilize under high pressure steam at 121°C for 20min). Then culture at 30°C and 180rpm for 48 hours, measure the pH value and OD value in the three bottles, and observe the number of bubbles in the medium, select the one with increased pH, high OD value and more bubbles to carry out subculture e...

Embodiment 2

[0059] The bacterial strain screened in Example 2 carried out 16S rDNA sequence analysis

[0060] The total DNA of the obtained strains was extracted, and then the genes were amplified and sequenced using 16S rDNA primers, and the sequencing results were submitted to Genbank for Blast comparison for homology analysis.

[0061] 16S rDNA gene amplification and PCR product analysis template preparation: the obtained pure strains were inoculated in the screening medium and cultured for 24 hours, the culture solution was collected by centrifugation to collect the precipitate, and the genome extraction kit was used (for the genome DNA extraction method, please refer to "Bacterial Molecular Genetics Classification and identification method" (Shanghai Science and Technology Press, 1990)) extract the total DNA of the bacteria, the fragment size is about 20kbp, the design of the 16S rDNA gene primer uses the general primer 27f / 1492r, provided by Guangzhou Huada Gene Company. The PCR rea...

Embodiment 3

[0067] Example 3 Aerobic denitrification and growth curves of Halomonas campisalis.BMEN3 at different salinities

[0068] Prepare denitration medium with NaCl concentration of 0, 2%, 6%, 12%, 18%, 24%, and 30% (less yeast powder compared with screening medium), pH 9, inject 100mL into a 250mL Erlenmeyer flask, Each concentration was repeated three times, sterilized at 121°C for 20 minutes, and then inoculated with 10% (take 10mL and centrifuge and wash three times with the culture medium) for small culture at 37°C and 180rpm, and sampled once every 4 hours, with three repetitions each time. Take a sample and measure the OD of the sample with a spectrophotometer 600 value, using ion chromatography (ICS-900, Dionex) to detect NO 3 - and NO 2 - concentration, the result is as figure 1 .

[0069] from figure 1 It can be seen that Halomonas campisalis.BMEN3 can grow at a salinity of 0-24%. From the growth curve and denitrification curve, it can be seen that the optimum growt...

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Abstract

The invention belongs to the field of microbe, and particularly relates to a halomonas campisalis with heterotrophic nitrification aerobic denitrification function and an application thereof. The preservation number of the halomonas campisalis BMEN3 (Halomonas campisalis. BMEN3) with heterotrophic nitrification aerobic denitrification function is CGMCC NO.6536. According to the halomonas campisalis BMEN3, the 16S rDNA sequence of the halomonas campisalis is shown as SEQ IDNo.1. The selected bacterial strain Halomonas campisalis. BMEN3 has heterotrophic nitrification and aerobic denitrification ability under the condition of high salinity and high alkali, and the bacterial strain can grow under the condition of aerobic heterotrophism, so that the growth velocity of the thallus is greatly improved, the biofilm culturing speed is enhanced, the starting time of the reactor is shortened, and the denitration efficiency is improved. The salinity and pH that the bacterial strain can adapt are wide in range, and the extreme value is high.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a salt-and-alkali-resistant Halomonas halomonas with heterotrophic nitrification-aerobic denitrification function and application thereof. Background technique [0002] With the development of industry and the continuous acceleration of urbanization, more and more nitrogen-containing waste gas and wastewater are discharged into the environment, and many of them not only contain high nitrogen content, but also have high salinity. Salt and nitrogen-containing wastewater mainly comes from wastewater directly utilized by seawater, such as cooling water in industries such as electric power, steel, and textiles, and seawater used for municipal administration. In addition, the flue gas produced by power plants, cremation plants, etc. also contains a large amount of nitrogen-containing compounds and oxygen. The absorption and removal of these nitrogen-containing compounds also need to be complete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/56B01D53/58C12R1/01C02F101/16
CPCY02A50/20
Inventor 邢建民郭岩杨茂华宋子煜
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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