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Heterotrophic nitrification-aerobic denitrification bacterium and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, applied in the field of microorganisms, to achieve good purification effect

Active Publication Date: 2017-08-11
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more and more types of heterotrophic nitrification-aerobic denitrification bacteria have been screened. The reported denitrification bacteria include Pseudomonas, Halomonasalkaliphila, Bacillus ), Acinetobacter (Xin Yufeng et al., 2011) and Alcaligenes, etc. At present, most scholars' research focuses on the isolation and screening of heterotrophic nitrification-aerobic denitrification bacteria and the characteristics of nitrogen removal However, there are still few reports on the application of heterotrophic nitrification-aerobic denitrification bacteria in actual seawater aquaculture.

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  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof
  • Heterotrophic nitrification-aerobic denitrification bacterium and application thereof

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Embodiment 1

[0020] Bacterial strain Alteromonas macleodii (Alteromonas macleodii) SLNX described in this patent 2 Screening From the sedimentary environment of sea cucumber breeding ponds in Hongdao, Qingdao, the mud samples were brought back to the laboratory and thoroughly stirred, then 10 g of mud samples were taken under aseptic conditions, put into 90 mL of sterile saline, and shaken for 10 min. Pipette 10 mL of supernatant to inoculate in liquid heterotrophic nitrification medium, and culture with shaking at 28°C and 150 rpm for 2 to 3 days. After repeated inoculation and culture for 3 times, take 10mL of the enrichment solution and dilute it appropriately, take 100μL and evenly spread it on the medium plate of heterotrophic nitrifying bacteria, put it in a constant temperature incubator, and incubate at 28°C for 2-3 days. Select single colonies of different shapes to separate by streaking on the plate, and repeat 2 to 3 times until pure single colonies are obtained. Then inoculate...

Embodiment 2

[0028] Strains: Three strains of heterotrophic nitrification-aerobic denitrification bacteria were screened and preserved by our laboratory, which have the function of heterotrophic nitrification-aerobic denitrification. Halomonas salkaliphila X 3 Screened from the eutrophic sedimentary environment in the cage fish culture area of ​​Xiangshan Port, where the ammonia nitrogen content is 42mg·L -1 In the test solution, the removal rates of ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen were 98.29%, 99.07%, and 96.48% respectively at 24 hours. Bacillus hwajinpoensis SLWX 2 Screened from the water environment of the Hongdao sea cucumber breeding pond in Qingdao City, the removal rates of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen reached 100%, 99.5% and 85.6% respectively in 24 hours, and can grow under the condition of 1%-8% NaCl

[0029] Alteromonas macleodii SLNX 2 Screened from the sedimentary environment of sea cucumber breeding ponds in Hongdao City...

Embodiment 3

[0059] Utilize three strains of heterotrophic nitrification-aerobic denitrification bacteria to carry out the biofilm method to treat the aquaculture water, and the biofilm method is to add an initial concentration of 10 5 CFU mL -1 The three strains of mixed bacteria liquid were added to the sterile seawater with nitrogen source and nitrogen source. The final concentration of the three strains of heterotrophic nitrifying-aerobic denitrifying bacteria in the mixed bacterial liquid was equal. The explosive cotton was used as the carrier and continuously aerated. The bacteria solution, nitrogen source and carbon source were supplemented every 5-7 days, and the film was successfully formed in 28 days. This biofilter can treat static and continuous flowing high-salt and high-nitrogen wastewater, keeping the concentration of inorganic nitrogen and organic matter in the water body at a low level, and the effect is better than that of the biofilter with natural membranes.

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Abstract

The invention provides a heterotrophic nitrification-aerobic denitrification bacterium and application thereof and belongs to the technical field of microorganisms. The bacterium is alteromonas macleodii SLNX2 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 27, 2015; and the preservation number is CGMCC NO. 11757. The invention further provides a method for purifying culture wastewater by utilizing the bacterium. The method specifically comprises the following step: controlling an adding final concentration of three heterotrophic nitrification-aerobic denitrification bacteria in a fish culture water body to be 1.3*10<6>CFU.mL<-1>. According to the method provided by the invention, the compound application and purification effect of the three bacteria is better than that of the single bacterium, so that ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen and chemical oxygen demand in the water body are lower than that of a control group without the bacterium and are lower than 68.55%, 48.36%, 58.38%, 40.02% and 27.47% respectively at maximum.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a heterotrophic nitrification-aerobic denitrification bacterium and its application. Background technique [0002] In recent years, the development of the aquaculture industry has realized the transformation from extensive to intensive. While the high-density aquaculture model brings high yield and high income, it also has the problem of rapid accumulation of organic matter such as residual bait and feces in the water body. The rapid increase of nitrogen concentration in aquaculture water has led to a serious decline in the self-purification capacity of the water body. Because harmful substances such as ammonia nitrogen and nitrite nitrogen are highly toxic to aquatic animals, they seriously affect the health and growth of aquatic economic animals and bring huge losses to the aquaculture industry. At the same time, the discharge of untreated high-nitrogen and high-organic aquacultu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20
CPCC02F3/34C02F2101/16C02F2101/163C02F2101/166C02F2103/20C12N1/205C12R2001/01
Inventor 李秋芬康传磊张艳王越孙雪梅成钰
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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