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Repair method of heavy metal polluted soil

A remediation method and technology for polluted soil, which are applied in the field of remediation of heavy metal contaminated soil, can solve problems such as difficulty in soil remediation work, and achieve the effects of remarkable purification effect, broad application prospect and promotion of removal effect.

Pending Publication Date: 2017-05-31
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of heavy metal-contaminated soil is imminent, but soil heavy metal pollution has the characteristics of concealment, long-term and irreversible, which increases the difficulty of soil restoration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 bacillus megaterium bacteria powder

[0025] 1. First, activate Bacillus megaterium; inoculate it into the seed medium (LB medium) according to the ratio of 2-5% for mixed culture; under the conditions of 35°C and 120rpm / min, shake it for 36-48 hours to obtain a grade seed liquid;

[0026] 2. According to the inoculation amount of 10-15%, transfer the primary seed solution to the secondary seed medium in the seed tank (each component and its mass ratio are: starch 1.5-3%, bran powder 1-2 %, yeast extract 0.05~0.3%, peptone 0.5~1%, phosphate rock powder 1~2%, MgSO4·7H2O 0.01~0.05%, CaCl2 0.1~0.4%, (NH4)2SO4 0.01~0.04%, balance water, pH 7.0-7.2), at 28-37°C, rotation speed 100rpm / min, ventilation rate 1000L / h, tank pressure maintained at 0.02-0.03MPa for 36-48h to obtain secondary seed liquid;

[0027] 3. According to the inoculum amount of 10-15%, inoculate the secondary seed solution into the fermentation medium in the fermenter (the c...

Embodiment 2

[0028] Example 2 Bacillus megaterium to the removal experiment of heavy metal in soil

[0029] Collect 10kg of mountain soil (almost free of free heavy metals) in Yangkou Scenic Area, Laoshan District, Qingdao City. The collected soil was air-dried naturally in a dry and ventilated place, ground through a 2mm aperture sieve, and mixed well.

[0030] Prepare polluted soil by artificially adding lead, chromium and copper at 2g / kg respectively: with Pb(NO3) 2 , Cr (NO3) 3 and Cu(NO3) 2 Add the solution into the soil and mix it evenly, put it in a ventilated place to dry naturally, pass it through a 2mm aperture sieve after aging for 60 days, and mix it well, the Pb in the soil 2+ 、Cr 3+ and Cu 2+ The contents are 2g / kg, 2g / kg and 2g / kg respectively. Weighed 200g of 8 mesh air-dried soils in 500mL triangular flasks, 1 treatment group added 10g hydroxyapatite (100 mesh); 2 treatment groups added 2g Bacillus megaterium powder (10 9 CFU / g); treatment group 3 was added with 10g...

Embodiment 3

[0038] The above results show that Bacillus megaterium bacteria powder can act synergistically with hydroxyapatite to effectively promote the fixation of free heavy metals, especially reduce the content of free heavy metals in the soil, and restore the soil polluted by heavy metals to a certain extent. The effect is remarkable. Application Broad prospects. Field experiment of embodiment 3 bacillus megaterium bacteria powder and hydroxyapatite to heavy metal polluted soil

[0039] The heavy metal-contaminated soil within 1 km near a large chemical plant in Sifang District, Qingdao was selected. After testing, the contents of various free heavy metals in the soil are shown in Table 4.

[0040] Table 4 Contents of free heavy metals in soil (mg / kg)

[0041] heavy metal lead chromium copper HG zinc cadmium content 811 898 526 1.9 305 1.5

[0042] 3.1 The first repair method: first add hydroxyapatite for balance treatment, and then add Bacillus...

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Abstract

The invention relates to the technical field of microbial applications, and in particular relates to a repair method of heavy metal polluted soil. According to the method provided by the invention, a soil environment is balanced by hydroxyapatite first, so that the removal effect of follow-up hydroxyapatite and bacillus megaterium powder on free state heavy metals can be effectively accelerated. One month later after on-site repair of the heavy metal polluted soil 1km near a chemical plant by adopting the method, the removal rates of free state lead, chromium, copper, mercury, zinc and cadmium in the polluted soil can separately reach 64.6%, 54.1%, 51.5%, 35.3%, 27.5% and 24.7%; two months later, the removal rates of free state lead, chromium, copper, mercury, zinc and cadmium in the polluted soil can separately reach 81.6%, 73.6%, 73.1%, 56.8%, 49.8% and 61.3%; second stage soil condition (GB15618-1995) formulated by the state is substantially met, that is, soil limit value on agricultural production and human health maintaining can be met. The repair method is obvious in purifying effect on the polluted soil and wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of functional microorganism application, in particular to a method for repairing heavy metal-contaminated soil. Background technique [0002] Soil heavy metal pollution not only degrades soil fertility, reduces crop yield and quality, deteriorates water environment, but also accumulates in humans and organisms through the food chain, seriously threatening human health. With the acceleration of industrialization, heavy metal pollution in soil has become more and more serious, and heavy metal pollution incidents are not uncommon. The treatment of heavy metal-contaminated soil is imminent, but soil heavy metal pollution has the characteristics of concealment, long-term and irreversible, which increases the difficulty of soil remediation. [0003] According to the different forms of heavy metals in the soil and the nature of the soil. The remediation and treatment methods of heavy metal-contaminated soil can b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/08B09C1/10
CPCB09C1/08B09C1/10B09C2101/00
Inventor 宗睿李佩佩刘鲁民周茜
Owner QINGDAO VLAND BIOTECH GRP
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