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Kandelia candel (L.) druce ERF (ethylene-responsive element binding factor) transcription factor as well as encoding gene, expression vector and application thereof

A technology of transcription factors and expression vectors, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve problems such as inability to plant stress resistance

Active Publication Date: 2011-08-31
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although a large number of genes related to stress resistance have been cloned from various plants, most of these genes can only increase a single resistance of plants, and cannot comprehensively improve the stress resistance of plants as a whole.

Method used

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  • Kandelia candel (L.) druce ERF (ethylene-responsive element binding factor) transcription factor as well as encoding gene, expression vector and application thereof
  • Kandelia candel (L.) druce ERF (ethylene-responsive element binding factor) transcription factor as well as encoding gene, expression vector and application thereof
  • Kandelia candel (L.) druce ERF (ethylene-responsive element binding factor) transcription factor as well as encoding gene, expression vector and application thereof

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Effect test

Embodiment 1

[0055] Example 1 The cloning, bioinformatics analysis and verification of structural domains of kandel ERF transcription factor (QqERF) gene

[0056] 1 Materials and methods

[0057] 1.1 Materials

[0058] 1.1.1 Plant material cultivation and stress treatment

[0059] The candela branches were collected from Shenzhen Mangrove Nature Reserve. The twigs were soaked in 250mM NaCl for 5 hours, and the young leaves were picked and immediately treated with liquid nitrogen, and stored in a -80°C ultra-low temperature refrigerator for later use.

[0060] 1.1.2 Strains and plasmids

[0061]

[0062] 1.1.3 Enzymes and reagents

[0063] Plant Total RNA Extraction Kit, DNA Gel Recovery Kit and High Purity Plasmid Miniprep Kit were purchased from Beijing Biotech Biotechnology Co., Ltd.; Taq enzyme, 3'full RACE Kit and 5'full RACE Kit were purchased from TaKaRa Company ; Restriction enzymes, T4 ligase, etc. were purchased from NEB Company; the first-strand cDNA synthesis kit was pur...

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Abstract

The invention discloses a kandelia candel (L.) druce ERF (ethylene-responsive element binding factor) transcription factor as well as an encoding gene, an expression vector and application thereof. In the invention, the nucleic acid sequence of the ERF transcription factor cDNA separated from mangrove plant kandelia candel (L.) druce is as shown in SEQ ID No.1. After the ERF transcription factor cDNA is transformed to tobacco, the contents of osmoregulation substances (such as proline) of a transgenic plant are obviously increased, and the salt tolerance of the transgenic plant is obviously improved. An experimental result indicates that the ERF transcription factor can response an adverse signal, thereby effectively improving environmental stress resistances of the plant such as salt andbase resistance, diseases and pets resistance and the like.

Description

technical field [0001] The present invention relates to a transcription factor cDNA related to plant stress resistance and its encoded protein, in particular to the cDNA sequence encoding the ERF transcription factor isolated and cloned from the mangrove plant Kandelia candel (L.) Druce, The invention also relates to plant high-efficiency expression vectors containing the cDNA sequence and their application in improving plant stress resistance, belonging to the field of transcription factors related to plant stress resistance. Background technique [0002] The stress of plants includes abiotic stress (such as drought, salinity and low temperature, etc.) and biological stress (such as diseases, insect pests and weed damage caused by fungi, bacteria, viruses and nematodes). Drought, salinity and low temperature are the three main abiotic stress factors that affect the growth of terrestrial plants and limit crop yield; among them, the impact of saline-alkali land on crop yield ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C07K14/415A01H5/00
Inventor 吴燕民赵毅萍赵杨敏庞俊峰尉亚辉李联社
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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