Exiguobacterium profundum and application thereof
A technology of microbacteria and seeds in the deep sea, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of incomplete enzymatic hydrolysis, easy blockage of pipelines, increased costs, etc., to promote fish growth, reduce feed coefficient, and increase energy consumption costs Effect
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Embodiment 1
[0033] Example 1: Screening and identification of deep-sea microbacteria P-47-3
[0034] The process of screening high-temperature bacteria producing protease is as follows: figure 1 shown. Firstly, the water samples obtained from the Okinawa hydrothermal area in Japan were isolated by the dilution coating method of MA2216E medium (Difco, product number: 212185), and a total of 42 strains were isolated. Through temperature experiments, 12 strains that can continue to grow at 50°C were initially screened out. Adopt protease screening culture medium again, point inoculation flat plate, observe after cultivating 3-5 days, it is positive to form transparent circle, screen to 6 strains of protease-producing bacterial strains, wherein the transparent circle of P-47-3 bacterial strain is the largest (such as figure 2 shown).
[0035] The protease screening medium was prepared according to the following proportions: (1) Weighed 4 g of commercially imported skim milk (Difco, produc...
Embodiment 2
[0046] Embodiment 2: the property of protease produced by deep-sea microbacterium P-47-3
[0047] The protease activity was measured by the Folin-phenol method, and the Folin-phenol reagent was purchased from Solebol, Cat. No. F8060.
[0048] Step 1: Make a Standard Curve
[0049] Take 14 test tubes and divide them into two groups, add 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1mL standard protein solution (250μg / mL) respectively, make up to 1mL with water, add 5mL reagent A, mix well, and store at 20-25℃ Let it stand for 10 minutes, then add 0.5mL reagent B, shake it up immediately, keep it warm at 20-25°C for 30 minutes, and then measure the color at 500nm. Measure the optical density value, get the mean value (table 1) of two groups of measurements, take the protein concentration as the abscissa, the optical density value as the ordinate, and draw the standard curve value ( Figure 6 ).
[0050] Table 1: Casein Standard Curve Data Sheet
[0051]
[0052] Step 2: Inoculate Exobacte...
Embodiment 3
[0055] Embodiment 3: Utilize deep-sea microbacterium P-47-3 fermentation to produce fermented fish paste
[0056] Step 1: Preparation of cell liquid culture of deep-sea microbacterium P-47-3: take the strains stored at -80°C and streak on LB solid medium, culture at 40°C, pick a single colony and inoculate it in a 25mL LB medium In a 100mL Erlenmeyer flask with base, the culture temperature was 40°C, and cultured on a shaker at a speed of 150r / min for 20-24h to the mid-logarithmic growth phase, and the cell liquid culture of deep-sea Exiguobacterium P-47-3 was obtained;
[0057] Step 2: Shake flask fermentation culture: the inoculum size is 2-5% (v / v), inoculated in a 500mL Erlenmeyer flask with 200mL LB medium, the culture temperature is 40°C, and the rotation speed is 150r / min on the shaker Under culture for 20-24h to obtain a fermentation broth.
[0058] Step 3: continue the shake flask fermentation culture: the inoculum size is 2-5% (v / v), inoculated in 1000mL LB liquid m...
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