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A BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene

A gene, birch technology, applied in the field of birch BpSPL9 gene and its encoded protein, can solve problems such as lack of

Inactive Publication Date: 2017-06-20
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, different transcription factors may lack a certain functional region, such as a specific DNA binding domain or a transcriptional regulatory domain, through which the transcription factor enters the nucleus at a specific time and interacts with the cis-acting element of the promoter or with other transcription factors. Interaction of functional domains to regulate transcriptional expression of genes

Method used

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  • A BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene
  • A BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene
  • A BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene

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specific Embodiment approach 1

[0024] Embodiment 1: The nucleotide sequence of the birch BpSPL9 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

[0025] This embodiment discloses the birch BpSPL9 gene and its encoded protein and its application. This embodiment constructs a plant expression vector containing the BpSPL9 gene, which uses the cauliflower mosaic virus (CaMV35S) promoter and adds a selectable marker card Namycin. The leaf disc transformation method mediated by Agrobacterium tumefaciens was used to genetically transform white birch, and the obtained transgenic white birch lines were analyzed for salt tolerance and drought resistance. The results showed that the transgenic lines were subjected to 1.0% NaCl and After 12h and 24h of PEG stress, the activities of these two enzymes were significantly higher than those of the non-transgenic control, indicating that the birch BpSPL9 gene can positively regulate the SOD activity and POD activity in plants, thereby enhancing the ...

specific Embodiment approach 2

[0026] Specific embodiment 2: The amino acid sequence of the protein encoding birch BpSPL9 gene in this embodiment is shown in SEQ ID NO: 2 in the sequence listing.

specific Embodiment approach 3

[0027] Specific embodiment three: The application of the birch BpSPL9 gene in this embodiment refers to the application in enhancing the stress resistance of plants.

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Abstract

The invention relates to a BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene and discloses the BpSPL9 gene of betula platyphylla Suk., the encoding protein thereof and the applications of the gene. The objective of the invention is to provide the BpSPL9 gene of betula platyphylla Suk., the encoding protein thereof and the applications of the gene. The nucleotide sequence of the gene is shown as SEQ ID NO:1 in a sequence table, and an amino sequence is shown as SEQ ID NO:2 in the sequence table. The gene can enhance adversity stress resistance of plants. An agrobacterium-tumefaciens-mediated leaf disc transformation method is utilized to perform genetic transformation of the betula platyphylla Suk., obtained transgenic betula platyphylla Suk. plants are subjected to salt resistance and drought tolerance analysis, and results show that the gene can enhance salt resistance and drought tolerance of the betula platyphylla Suk.. Through the gene, the encoding protein and the applications, a novel BpSPL9-transgenic betula platyphylla Suk. variety that is salt-resistant and drought-tolerant can be cultivated. The gene, the encoding protein and the applications are applied in the field of forest tree molecular breeding.

Description

technical field [0001] The invention relates to birch BpSPL9 gene and its encoded protein and application. Background technique [0002] Transcription factors (transcription factors, TFs), also known as trans-acting factors, refer to those protein molecules that specifically bind to cis-acting elements in the promoter region of eukaryotic genes, or bind to the structural characteristics of known DNA binding domains, It has the function of activating or inhibiting gene transcription effect. The number of transcription factors in higher plants is large, there are many types, and the transcription regulation is relatively complex. The research on transcription factors has attracted more and more attention. At present, a large number of transcription factors related to the regulation of drought, high salinity, low temperature, hormones, pathogenic response and growth and development have been isolated from higher plants. [0003] A typical plant transcription factor generally ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00
Inventor 李慧玉宁坤董京祥姜静黄海娇陈肃
Owner NORTHEAST FORESTRY UNIVERSITY
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