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Salt-tolerant halomonas strain and application thereof in field of water purification

A technology of Halomonas and strains, applied in the field of environmental microorganisms, can solve problems such as difficulty in exerting advantages and few salt tolerance characteristics

Active Publication Date: 2022-04-08
青岛蔚蓝赛德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, few of the currently screened strains have salt-tolerant characteristics, making it difficult for them to exert their advantages in the application of high-salt wastewater. Therefore, it is of great significance to screen high-salt-tolerant strains to solve the problem of high-salt wastewater treatment.

Method used

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  • Salt-tolerant halomonas strain and application thereof in field of water purification
  • Salt-tolerant halomonas strain and application thereof in field of water purification
  • Salt-tolerant halomonas strain and application thereof in field of water purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. strain screening and performance testing

[0038] 1. Enrichment culture

[0039]Collect sewage from a fermentation plant, absorb 10ml of sewage and transfer to 100ml enrichment medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, 50g / L sodium chloride, solvent In a 250ml Erlenmeyer flask with water adjusted to pH=7.0), cultivate under the conditions of 30°C and 200r / min for the first enrichment. When the ammonia nitrogen content drops to the lowest level, absorb another 10ml of the primary enrichment solution, add it to a fresh 100ml enrichment medium, and culture at 30°C and 200r / min for the second enrichment. Carry out the third enrichment according to the above enrichment method.

[0040] 2. Primary screening

[0041] The third enrichment solution was diluted to 10 by gradient dilution method -3 、10 -4 、10 -5 and 10 -6 , Take 200 μl of each dilution on a nutrient agar plate, spread it evenly, and then place it upside down ...

Embodiment 2

[0049] Example 2. Detection and Identification of Halomonas AOB-NY01 Strain

[0050] The slant of the AOB-NY01 strain was sequenced by 16S rDNA gene sequence, and the sequencing results were compared in NCBI (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROGRAM=blastn&PAGE_TYP E=BlastSearch&LINK_LOC=blasthome), select The sequence with the highest similarity was used as the species identification result (see the analysis comparison results in Table 2 for details).

[0051] NCBI comparison results of samples in Table 2

[0052] ID No. DNA identification results identities AOB-NY01 Halomonas sp.GT 99％

Embodiment 3

[0053] Example 3. Halomonas tolerance to different environmental conditions

[0054] 1. Growth of Halomonas AOB-NY01 strain at different salinities

[0055] In a sterile environment, inoculate the AOB-NY01 strain into 100ml enriched medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, 50g / L sodium chloride, solvent be water, adjust pH=7.0) in a 250ml Erlenmeyer flask, cultivate for 48h under 30°C and 200r / min conditions for activation to obtain an activation solution;

[0056] Draw 5 μl of activation solution to inoculate into 100ml of sterilized evaluation medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, solvent is water, adjust pH=7.0) In a 250ml Erlenmeyer flask, the salinity of the evaluation medium is 0-30%, cultured at 30°C and 200r / min for 24 hours, and the absorbance OD600 is measured respectively (OD600 represents the absorbance value of the solution at 600nm wavelength, which is used to determine Measuring t...

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Abstract

The invention relates to a salt-tolerant Halomonas sp. Strain and application thereof in the field of water purification, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.24127. The strain has good salt tolerance, the optimum growth salinity is 5%, under laboratory conditions, the ammonia nitrogen degradation rate reaches 100% in 24 h when the salinity is 15% or below, and the ammonia nitrogen degradation rate reaches 100% when the salinity is 15% or below. The strain can resist high ammonia nitrogen concentration and high temperature, the ammonia nitrogen degradation rate reaches 89.1% within 72 h for the ammonia nitrogen concentration of 3000 ppm, and when the initial ammonia nitrogen concentration is 100 ppm, the ammonia nitrogen degradation rate reaches 100% within 24 h at the temperature of 30-40 DEG C.

Description

technical field [0001] The present invention relates to a Halomonas sp. strain and a microbial agent containing it, in particular to a Halomonas sp. The field of environmental microbiology technology. Background technique [0002] Eutrophication of water body is a large category of water pollution, of which ammonia nitrogen pollution accounts for a large proportion, so it has aroused people's close attention. The denitrification methods in sewage treatment mainly include physical chemical method and biological method. Physicochemical methods include breakpoint chlorination method, ion exchange method, etc. The cost of sewage treatment is high, and it has a great impact on the environment, while the biological method has low cost, strong denitrification ability, and no secondary pollution to the environment. Secondary pollution, with high practical value. [0003] However, printing and dyeing, pesticides, coal chemical industry, papermaking, oil refining, seawater utiliz...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCY02W10/10
Inventor 朱威刘圣鹏
Owner 青岛蔚蓝赛德生物科技有限公司
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