Halotolerant bacterium separation and purification method, saline-alkaline tolerant strain obtained from separation and application of saline-alkaline tolerant strain

A technology for the separation and purification of salt-tolerant bacteria, applied in the field of microorganisms, it can solve the problems of microbial separation without salinity and alkali, and achieve the effects of excellent soil vitality, ideal screening results, and quick results.

Active Publication Date: 2017-02-22
本农合众科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no effective technology for the isolation and purification of microorganisms in saline-alkali land in the prior art

Method used

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  • Halotolerant bacterium separation and purification method, saline-alkaline tolerant strain obtained from separation and application of saline-alkaline tolerant strain
  • Halotolerant bacterium separation and purification method, saline-alkaline tolerant strain obtained from separation and application of saline-alkaline tolerant strain
  • Halotolerant bacterium separation and purification method, saline-alkaline tolerant strain obtained from separation and application of saline-alkaline tolerant strain

Examples

Experimental program
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Effect test

Embodiment 1

[0059] The present embodiment provides a kind of microbial screening method, and this method comprises the steps:

[0060] 1) Dilute the saline-alkali soil sample at 1:1000 and inoculate it in the nutrient broth enrichment medium. Under the condition of 30°C, carry out aerobic constant temperature culture shaker culture at 200rpm, and cultivate for 3 days to obtain mixed bacteria liquid;

[0061] The nutrient broth enrichment medium comprises the following components: peptone 8g / L, beef extract 4g / L, sodium chloride 4g / L; solvent is water;

[0062] 2), inoculate the mixed bacterial solution onto a nutrient broth agar plate for streak culture, and pick colonies conforming to the form of bacillus therefrom;

[0063] The single colony of Bacillus is generally white in shape, irregular around, protruding in the middle, Gram-positive, and transparent spores appear when observed under a microscope.

[0064] The medium in the nutrient broth agar plate includes the following compone...

Embodiment 2

[0075] The present embodiment provides a kind of microbial screening method, and this method comprises the steps:

[0076] 1) Dilute the saline-alkali soil sample at 1:1000 and inoculate it in the nutrient broth enrichment medium. Under the condition of 30°C, carry out aerobic constant temperature culture shaker culture at 200rpm, and cultivate for 3 days to obtain mixed bacteria liquid;

[0077] The nutrient broth enrichment medium comprises the following components: peptone 12g / L, beef extract 2g / L, sodium chloride 6g / L; solvent is water;

[0078] 2), inoculate the mixed bacterial solution onto a nutrient broth agar plate for streak culture, and pick colonies conforming to the form of bacillus therefrom;

[0079] The single colony of Bacillus is generally white in shape, irregular around, protruding in the middle, Gram-positive, and transparent spores appear when observed under a microscope.

[0080] The medium in the nutrient broth agar plate includes the following compon...

Embodiment 3

[0091] The present embodiment provides a kind of microbial screening method, and this method comprises the steps:

[0092] 1) Dilute the saline-alkali soil sample at 1:1000 and inoculate it in the nutrient broth enrichment medium. Under the condition of 30°C, carry out aerobic constant temperature culture shaker culture at 200rpm, and cultivate for 3 days to obtain mixed bacteria liquid;

[0093] The nutrient broth enrichment medium comprises the following components: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L; solvent is water;

[0094] 2), inoculate the mixed bacterial solution onto a nutrient broth agar plate for streak culture, and pick colonies conforming to the form of bacillus therefrom;

[0095] The single colony of Bacillus is generally white in shape, irregular around, protruding in the middle, Gram-positive, and transparent spores appear when observed under a microscope.

[0096] The culture medium in the nutrient broth agar plate includes the followin...

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Abstract

The invention relates to the field of microorganisms, and in particular to a halotolerant bacterium separation and purification method, a saline-alkaline tolerant strain obtained from separation and an application of the saline-alkaline tolerant strain. Bacillus amyloliquefaciens, which is selected by the invention, is a microorganism which is extracted and screened from saline-alkali soil in Hetao area and is obvious in saline-alkaline tolerance, and the microorganism is excellent in capacity of improving soil activity in the Hetao area. According to the screening method provided by the invention, the microorganism, which is high in salt-tolerance capacity, is screened from a test soil sample by virtue of an enrichment and separation culture mode; the screening method has the effects of being strongly targeted and ideal in screening result, so as to guarantee saline-alkaline soil remediation which is short in cycle and rapid to take effects.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for isolating and purifying salt-tolerant bacteria, the isolated salt-alkali-resistant bacterial strain and applications thereof. Background technique [0002] The problem of soil salinization is one of the most serious environmental problems in the world at present. Various factors, including population expansion, continue to cause human beings to develop and utilize large areas of land to produce new secondary salinization and accelerate the process of soil salinization. [0003] The saline-alkali soil contains excessive salinity, toxic substances, excessive alkalinity and poor soil physical properties, which can inhibit the growth of plants and lead to the deterioration of regional ecology. Under the premise of promoting ecological benefits, biological improvement measures have become a research hotspot, and the improvement effect of microorganisms on saline-alkali land ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/04C09K17/14C12R1/07C09K101/00
CPCC09K17/14C09K2101/00C12N1/02C12N1/20C12Q1/04C12N1/205C12R2001/07
Inventor 赵川韩建均王玉文
Owner 本农合众科技有限公司
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