Preparation method and application of jasmine plant leaf cell extract

A technology for plant leaves and jasmine flowers, which is applied in the field of preparation of jasmine flower leaf cell extracts, can solve the problems of serious loss of polyphenols, low selectivity of extracts, difficult operation and control, etc., achieves product safety, shortens extraction time, The effect of lowering the boiling point of extraction

Inactive Publication Date: 2015-05-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Microwave extraction method: the time is too short, the operation is not easy to control, and the loss of polyphenols is relatively serious
Thermal extraction method: use heat as conduction, heat radiation, etc. from the outside to the inside, thermal extraction method: low extraction rate, low yield, low selectivity to extracts, and long time-consuming

Method used

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  • Preparation method and application of jasmine plant leaf cell extract
  • Preparation method and application of jasmine plant leaf cell extract
  • Preparation method and application of jasmine plant leaf cell extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Pretreatment of samples: pick jasmine leaves, cut them into pieces, use 40 mesh to crush them, and set aside.

[0027] (2) Weigh ethanol 10 times the weight of jasmine plant leaves and mix them with jasmine plant leaves in a flask to form a feed solution. The vacuum degree in the system is 0.6MPa, and the temperature is 55°C and the ultrasonic power is 420W. React for 12min, the mass fraction It is 70% ethanol, and after the whole extraction is completed, the tea polyphenol extract is collected by filtration, which is marked as sample 1.

[0028] (3) Determination of tea polyphenols content: adopt high performance liquid chromatography to carry out the mensuration of tea polyphenols content (, its analysis time is short, easy to operate. Its optimal analysis condition is: methyl alcohol: water: acetic acid=20:60: 0.4 (volume ratio), flow rate is 1.1ml / min, injection volume: 5×10 -3 , the temperature is 25°C, and the wavelength of the ultraviolet detection wave is 2...

Embodiment 2

[0038] (1) Pretreatment of samples: pick jasmine leaves, cut them into pieces, use 60 mesh to crush them, and set aside.

[0039] (2) Take by weighing 15 times of jasmine plant leaf weight ethanol and jasmine plant leaves to mix in a flask to form a feed liquid, connect the equipment, turn on the vacuum pump to make the whole system form a negative pressure, the vacuum degree in the system is 0.4MPa, at a temperature of React at 50°C and ultrasonic power of 420W for 1min, and the mass fraction of ethanol is 70%. After the whole extraction is completed, the tea polyphenol extract is collected by filtration, which is marked as sample 2.

[0040] Determination of the content of propylene glycol, a lipid peroxidation product, on sample 2: Determination of the content of propylene glycol, a lipid peroxidation product, in vitro tissue homogenate of Kunming mice and different concentrations of tea polyphenols;

[0041] Experimental grouping: 270 Kunming mice were divided into 3 group...

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Abstract

The invention discloses a preparation method and an application of a jasmine plant leaf cell extract. The preparation method comprises the following steps: (1) selecting jasmine plant leaves as raw materials; (2) weighing ethanol 10-15 times as much as the weight of the jasmine plant leaves, mixing the ethanol with the jasmine plant leaves to prepare feed liquid, and putting the feed liquid in an environment with a vacuum degree of 0.4-0.6 MPa, reacting for 1-15 minutes at a temperature of 50-60 DEG C and ultrasonic power of 420W; (3) filtering and collecting to obtain the jasmine plant leaf cell extract. In the invention, the jasmine plant leaf cell extract is extracted by a vacuum coupling ultrasonic extraction method, the extraction time is shortened, the product yield is high, the working efficiency is high and the product is safe; the jasmine plant leaf cell extract is added in such cosmetics as facial cream to achieving anti-aging and anti-ultraviolet beautifying effects.

Description

technical field [0001] The invention belongs to the field of biological cosmetology, and in particular relates to a preparation method and application of a jasmine leaf cell extract with cosmetic effect. Background technique [0002] Jasmine is a plant of Mirabilis mirabilis, commonly known as mine flower, rouge flower, pink bean flower, and night meal flower. Leaves are opposite, simple, papery, round, oval, ovate-elliptic or obovate, with rounded or blunt ends, sometimes slightly cordate base, slightly concave or concave above, convex below, thin Veins are often conspicuous on both sides, slightly raised, glabrous except for tufts in the axils of lower veins; lobes oblong to nearly round, apex round or obtuse. Fruit spherical, purple-black. The flowering period is from May to August, and the fruiting period is from July to September. Perennial herbaceous flowers, often used as annual cultivation in production. Mirabilis is not only a good flower that can play a role in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08A61Q19/02A61Q19/00A61Q17/04
Inventor 陈海佳王一飞葛啸虎黎敏聪池诗源
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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