Magnesium-nitrogen-doped carbon dots, preparation method thereof and application of magnesium-nitrogen-doped carbon dots in improvement of photosynthesis of plants

A carbon dot, magnesium nitrogen technology, applied in the field of nanomaterial biological effect research, can solve the problems of limited light capture ability, photosynthetic system activity, low plant photosynthesis efficiency, etc., to promote synthesis and metabolism, improve photosynthetic activity, and accelerate growth. Effect

Active Publication Date: 2021-08-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the photosynthetic efficiency of plants is much lower than its theoretical value, and the main limiting factors include: (1) the limited light harvesting ability of plants themselves and (2) limited photosynthetic system activity

Method used

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  • Magnesium-nitrogen-doped carbon dots, preparation method thereof and application of magnesium-nitrogen-doped carbon dots in improvement of photosynthesis of plants
  • Magnesium-nitrogen-doped carbon dots, preparation method thereof and application of magnesium-nitrogen-doped carbon dots in improvement of photosynthesis of plants
  • Magnesium-nitrogen-doped carbon dots, preparation method thereof and application of magnesium-nitrogen-doped carbon dots in improvement of photosynthesis of plants

Examples

Experimental program
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Effect test

Embodiment 1

[0030]Preparation of magnesium nitrogen doped carbon dots (Mg,N-CDs): Mg,N-CDs were prepared using citric acid, ethanolamine and magnesium hydroxide as raw materials by a one-step hydrothermal method, and the molar ratio was 1:0.2:0.35 Citric acid, ethanolamine and magnesium hydroxide were dissolved in ultrapure water, and ultrasonically treated for 30 minutes. Magnesium hydroxide, which is slightly soluble in water, can be completely dissolved due to the chelating effect of citric acid. Then, the mixed solution obtained by ultrasonic treatment was poured into a 50 mL polytetrafluoroethylene reactor lining, loaded into the reactor, and reacted at 200° C. for 6 h. After the reaction kettle was lowered to room temperature, the brown product was filtered through a filter head with a pore size of 0.22 μm, and then dialyzed with a dialysis bag (MW 100-500) for 12 hours, and the dialysate was ultrapure water. After freeze-drying, magnesium-nitrogen doped carbon dots (Mg, N-CDs) can...

Embodiment 2

[0033] The magnesium nitrogen doped carbon point (Mg, N-CDs) obtained in embodiment 1 is characterized:

[0034] Characterization method: The morphology of Mg, N-CDs was observed by JEM-2100F field emission transmission electron microscope (TEM) and SPA-400 atomic force microscope (AFM). Characterization was performed using a Thermo Scientific Nicolet 6700FTIR spectrometer and a Thermo Scientific K-Alpha X-ray photoelectron spectroscopy (XPS). The fluorescence emission spectra and fluorescence lifetime decay curves of Mg, N-CDs were measured by F-7000Hitachi and FLS1000-stm fluorescence spectrometers, respectively. A PerkinElmer UV-Vis spectrophotometer (Lambda 750) was used to measure the UV-Vis light absorption curve of Mg, N-CDs. At the same time, we also used TGA-DSC thermogravimetry (Labsys STA) coupled with mass spectrometry (LC-DZOOM Pro) (TG-MS) to determine the thermal decomposition curve and product composition of Mg, N-CDs from room temperature to 800 °C. Finally,...

Embodiment 3

[0042] Effects of the magnesium and nitrogen doped carbon dots (Mg, N-CDs) obtained in Example 1 on the growth of rice plants:

[0043] The treatment method of magnesium nitrogen doped carbon dots on rice plants: first use 75% ethanol solution to sterilize the rice seeds (Huahang No. 31), wash thoroughly with deionized water, spread them evenly in the germination tray, and add 50% concentration of nutrients cultured at 30°C in the dark. After 3 days, the cover of the germination tray was uncovered, and the seeds germinated substantially completely, and were placed in a greenhouse (22-30° C., 70% relative humidity, 400 μmol m -2 ·s -1 The light intensity, the photoperiod is 16h light / 8h dark) and continue to cultivate until the 7th day. Rice seedlings with uniform growth state were selected and transplanted into black square plastic boxes, 12 plants per box, and the nutrient solution in the box was replaced twice a week. Different concentrations of Mg,N-CDs (50, 100 and 300 ...

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Abstract

The invention belongs to the field of nano material biological effect research, and discloses a magnesium-nitrogen-doped carbon dot, a preparation method thereof and application of the magnesium-nitrogen-doped carbon dot in improvement of plant photosynthesis. The preparation method comprises the following steps: dissolving citric acid, ethanolamine and magnesium hydroxide in ultrapure water, carrying out ultrasonic treatment, pouring the treated system into a reaction kettle, and conducting reacting at 200 DEG C for 6 hours; after the reaction is finished, conducting cooling to room temperature, filtering a product by using a filter head with the pore diameter of 0.22 [mu]m, and then conducting dialyzing for 12 hours; and conducting freeze-drying to obtain the magnesium-nitrogen doped carbon dots. After the carbon dots are compounded with chloroplast, the excitation energy can be absorbed by the chloroplast for photosynthesis, so that the photosynthetic activity is improved. After the carbon dots are applied to rice plants through foliage spraying, Mg,N-CDs can be uniformly distributed in leaf cells, expression of related enzyme genes is regulated and controlled, synthesis and metabolism of chlorophyll in leaves are promoted, the activity of chlorophyll molecules is kept at a high level, and therefore the photosynthetic activity of the leaves is improved. Finally, the plant growth is obviously improved.

Description

technical field [0001] The invention belongs to the field of biological effect research of nanometer materials, and specifically relates to a magnesium-nitrogen-doped carbon point, a preparation method thereof, and an application in improving plant photosynthesis. Background technique [0002] Photosynthesis is an important physiological process in the process of plant growth, which is of great significance to food production. Improving the yield of crops by regulating photosynthesis has become a hot topic for many researchers. At present, the photosynthetic efficiency of plants is far lower than its theoretical value, and the main limiting factors include: (1) the limited light harvesting ability of plants themselves and (2) limited photosynthetic system activity. As light harvesters, photosynthetic pigments are involved in almost all physiological processes of plant photosynthesis, including: light harvesting, energy transfer, energy conversion, etc. Magnesium and nitrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15A01G7/06
CPCC09K11/65C09K11/0883B82Y20/00B82Y40/00C01B32/15A01G7/06
Inventor 刘应亮李亚东胡超凡庄健乐雷炳富张学杰许晓凯郑明涛李唯张浩然
Owner SOUTH CHINA AGRI UNIV
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