Separation and purification method for Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide

A technology of separation and purification of leaves of black rice, applied in the directions of digestive system, metabolic diseases, antiviral agents, etc., to achieve the effect of promoting development, no drug residues, and enhancing immune function

Inactive Publication Date: 2016-07-20
JIANGSU CHANGYUN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that blueberry leaves are rich in plant polysaccharides, but there a

Method used

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  • Separation and purification method for Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 (Isolation and Purification of Polysaccharides from Black Rice Leaf)

[0021] 1) The cell wall of the leaves of Wufan tree is broken. With black rice leaves as raw material. First, take 10Kg of fresh black rice leaves and clean them; secondly, use the ultrasonic crushing method to break the cell wall of the leaves. Purified water with a weight ratio of 1:1 enters the colloid mill for grinding, selects the grinding fineness of 10 μm, and grinds 5 times; finally, freezes the ground product at -15°C for 1 hour, and then puts it in 80°C water to heat up suddenly It melts and heats up for 15 minutes, and after the ground product is cooled to room temperature, it is frozen at -15°C for 1 hour, and this is repeated 3 times to obtain the broken cell wall liquid of the leaves of the black rice plant.

[0022] 2) Coarse separation of polysaccharides from leaves of black rice leaves. First, using a KOH solution with a weight concentration of 1%, according to the sta...

Embodiment 2

[0024] Embodiment 2 (isolation and purification of blueberry leaf polysaccharide)

[0025] 1) The cell wall of the blueberry leaf is broken. Made with blueberry leaves. First, take 10Kg of fresh blueberry leaves and clean them; secondly, use the ultrasonic crushing method to break the cell wall of the leaves. Purified water with a ratio of 1:1 enters the colloid mill for grinding, selects the grinding fineness of 10 μm, and grinds 5 times; finally, freezes the ground product at -15°C for 1 hour, then puts it in 80°C water and suddenly heats it up to make it Melt, heat up for 15 minutes, after the ground product is cooled to room temperature, put it into -15°C environment and freeze it for 1 hour, and repeat this process 3 times to obtain the blueberry leaf broken cell wall liquid.

[0026] 2) Coarse separation of blueberry leaf polysaccharides. First, using a KOH solution with a weight concentration of 1%, according to the standard that the mass ratio of the KOH solution ...

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Abstract

The invention discloses a separation and purification method for Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide. The method includes the steps that fresh Vaccinium bracteatum Thunb. leaves and blueberry leaves are adopted as raw materials, cell disruption is carried out through the ultrasonic technology, extraction of polysaccharide is carried out with an alkaline solution, crude polysaccharide purification is carried out with composite biological enzymes, and pure natural plant polysaccharide ingredients are obtained. The method includes the four steps of Vaccinium bracteatum Thunb. leaf and blueberry leaf cell wall disruption, primary separation of Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide, purification of Vaccinium bracteatum Thunb. leaf polysaccharide and purification of blueberry leaf polysaccharide. The Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide products obtained after separation and purification with the method mainly have the effects of growth and development promotion, virus resistance, immune function enhancement, regulation and control of the intestinal microecological environment and promotion of development of immune organs on the human body.

Description

technical field [0001] The invention belongs to the technical field of plant nutrient extraction, and in particular relates to a method for separating and purifying polysaccharides from black rice leaves and blueberry leaves. Background technique [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. Plant polysaccharides have biological activity, and have health effects on the human body such as immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-radiation, antibacterial, antiviral, and liver protection. [0003] Plant polysaccharides have the function of storing bioenergy (such as: starch, glycogen, inulin) and supporting structure (such as: cellulose, chitin, mucopolysaccharide). However, the polysaccharide components of the cell membrane and cell wall are not only supporting substances, but also directly participate in the cell division proce...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/12A61P37/04A61P1/14A61P1/00A61P3/00
CPCC08B37/0003
Inventor 蒋冠伟
Owner JIANGSU CHANGYUN BIOTECH CO LTD
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