Method for extracting wild persimmon leaf tannin by ultrasonic wave auxiliary semi-bionic method

A technology of persimmon leaves and semi-bionic method, which is applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of high extraction cost, unsuitable for biological agents, VOC pollution, etc., and achieve shortened extraction time and no environmental pollution. Pollution, the effect of maintaining activity

Inactive Publication Date: 2012-02-08
余先纯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The water-bath reflux method mainly has the following disadvantages when extracting persimmon tannins: complex process, low tannin yield; long extraction time, multiple extractions are required, and high extraction cost; there are many by-products in the product, and the separation is complicated; the activity of tannin Low, not suitable for making biological agents, which affects the use in biopharmaceuticals; due to the use of a large amount of organic solvents, it is easy to cause VOC pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example uses 3 extractions, then the extracts are mixed and concentrated to dryness.

[0042] The first extraction: Accurately weigh and dry persimmon leaf powder of 30 meshes and crush it into 100g of wild persimmon leaf powder, add 300g of extraction solvent (the mass ratio of ethanol to water is 1:1.5), adjust the pH value to 2.0 with acetic acid, and heat in a water bath to 50°C, heat preservation and extraction for 40 minutes. During this period, ultrasonic waves with a power of 200W and a frequency of 26 kHz were used every 5 minutes to assist the semi-bionic extraction. The use time was 5 minutes each time, a total of 20 minutes. An extract containing tannins is obtained after filtration.

[0043] The second extraction: Add 200g of extraction solvent (mass ratio of ethanol to water: 1:2) to the above-mentioned filtered wild persimmon leaf residue, adjust the pH value to 7.2 with sodium carbonate, heat the water bath to 50°C to start insulation extraction Fo...

Embodiment 2

[0047] This example uses 3 extractions, then the extracts are mixed and concentrated to dryness.

[0048] First extraction: Accurately weigh and dry persimmon leaf powder of 30 meshes and crush it into 100g of wild persimmon leaf powder, add 300g of extraction solvent (the mass ratio of ethanol to water is 1:1.5), adjust the pH value to 2.1 with acetic acid, and heat in a water bath to 55 °C, heat preservation and extraction for 40 minutes. During this period, ultrasonic waves with a power of 250W and a frequency of 29 kHz were used every 10 minutes to assist the semi-bionic extraction. The use time was 10 minutes each time, a total of 20 minutes. An extract containing tannins is obtained after filtration.

[0049] The second extraction: add 200g of extraction solvent (the mass ratio of ethanol to water is 1:2) to the above-mentioned filtered wild persimmon leaf residue, adjust the pH value to 7.4 with sodium carbonate, heat the water bath to 55 ° C to start the insulation ext...

Embodiment 3

[0053] This example uses 3 extractions, then the extracts are mixed and concentrated to dryness.

[0054] The first extraction: Accurately weigh and dry persimmon leaf powder of 30 mesh, add 300g of extraction solvent (the mass ratio of ethanol to water is 1:3), adjust the pH value to 2.2 with acetic acid, and heat in a water bath to 60 °C, heat preservation and extraction for 40 minutes. During this period, ultrasonic waves with a power of 300W and a frequency of 32 kHz were used every 10 minutes to assist the semi-bionic extraction. The use time was 10 minutes each time, a total of 20 minutes. An extract containing tannins is obtained after filtration.

[0055] The second extraction: Add 200g of extraction solvent (the mass ratio of ethanol to water is 1:2) to the above-mentioned filtered wild persimmon leaf residue, adjust the pH value to 7.5 with sodium carbonate, heat the water bath to 60°C and start the insulation extraction For 40 minutes, ultrasonic waves with a power...

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Abstract

The invention relates to a method for extracting tannin from wild persimmon leaves by an ultrasonic wave auxiliary semi-bionic method, which is mainly characterized in that the de-enzymed wild persimmon leaves are dried and smashed, ethanol and water as used as extraction solvents, and backflow extraction is carried out 3 times at the temperature of 50-60 DEG C by simulating the pH value of a gastrointestinal tract; and in the process of each extraction, ultrasonic waves are adopted for auxiliary treatment; and extracts in three times are combined and centrifuged, and then the obtained extract is concentrated and dried in a vacuum mode at the temperature of 50-60 DEG C to obtain the wild persimmon leaf tannin. In the semi-bionic extraction method of the invention, ultrasonic wave auxiliary extraction is adopted to make wild persimmon leaf cell tissues be wall-broken or deformed so as to increase the tannin yield, shorten the extraction time and keep the activity of the tannin.

Description

technical field [0001] The invention relates to a method for extracting tannins from wild persimmon leaves, in particular to a method for extracting tannins from wild persimmon leaves by an ultrasonic-assisted semi-bionic method. Background technique [0002] Tannins, also known as tannic acid, tannin, is a water-soluble substance with a polyphenolic hydroxyl structure existing in plants. It has strong biological activity, excellent antioxidant and free radical scavenging, etc. Function, can chelate with metal ions, and can react with proteins, alkaloids, polysaccharides, etc., thus exhibiting strong antioxidant capacity, excellent antibacterial, antiviral activity and inhibition of carcinogens and tumor activity. It has unique advantages in preventing and treating diseases caused by free radicals, inhibiting the peroxidation of lipids and other substances, and resisting AIDS. It is widely used in medicine, food, leather, and printing and dyeing industries. [0003] At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 余先纯孙德林李湘苏
Owner 余先纯
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