Method for determining plant leaf cell conveying capacity

A plant leaf and cell transport technology, applied in the fields of agricultural engineering and crop information detection, can solve the problem of no method for measuring plant leaf cell transport capacity, and achieve the effects of low price, online quantitative detection, and wide applicability

Active Publication Date: 2018-09-04
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the method for measuring the cell transport capacity of plant leaves

Method used

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  • Method for determining plant leaf cell conveying capacity
  • Method for determining plant leaf cell conveying capacity
  • Method for determining plant leaf cell conveying capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Take framing trees as an example. On the campus of Jiangsu University, the leaves with relatively consistent growth on fresh branches were picked, and they were quickly returned to the laboratory. After cleaning the surface dust of the leaves on the fresh branches, the leaves were immediately placed in ① pH 7.0 solution for 4 hours, ② pH 7.0 solution Treat for 6 hours, ③ pH 9.0 solution for 4 hours, connect the measuring device to the LCR tester, clean the leaves, clamp the above-mentioned treated leaves between parallel plates, set the measuring voltage to 1.5 volts, and the measuring frequency to 3000 Hz, parallel mode Measure the physiological resistance, physiological impedance, and physiological capacitance (such as Table 1) of the leaves of the mulberry plant; then, according to the formula: (Wherein Xc is the physiological capacitance of the tree leaf, C is the physiological capacitance of the leaf of the tree, f is the test frequency, and π is the pi equal to 3...

Embodiment 2

[0053] Take the mulberry tree, for example. On the campus of Jiangsu University, pick leaves with consistent growth on fresh branches, and quickly return to the laboratory. After cleaning the surface dust on the leaves on the fresh branches, the leaves are immediately placed in ① pH 5.0 solution for 2 hours, ② pH 6.0 solution Treat for 2 hours, ③pH 7.0 solution for 2 hours, ④pH 8.0 solution for 2 hours, ⑤pH 9.0 solution for 2 hours, ⑥pH 5.0 solution for 4 hours, ⑦pH 6.0 solution for 4 hours, ⑧pH 7.0 solution for 4 hours ⑨pH is 8.0 solution treatment for 4 hours, ⑩pH is 9.0 solution treatment for 4 hours; the measuring device is connected with the LCR tester, the blade is cleaned, the blade after the above treatment is clamped between parallel plates, the measuring voltage is set to 1.5 volts, and the measurement Frequency is 3000Hz, parallel mode measures mulberry plant leaf physiological resistance, physiological impedance, physiological capacitance (as table 4); Then, accord...

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Abstract

The invention discloses a method for determining a plant leaf cell conveying capacity, and belongs to the technical field of agricultural engineering and crop information detection. A determining device comprises a support, foam plates, electrode plates, lead wires, an iron block and a plastic rod; the foam plates with the inlaid electrode plates are adhered onto the bottom end of the support andthe plastic rod; when in use, the method comprises the steps of connecting the electrode plates and an LCR tester through the lead wires; clamping a plant leaf to be measured through the two electrodeplates, determining the physiological resistance, physiological impedance and physiological capacitance of the plant leaf through a parallel-connection mode, and calculating physiological capacitivereactance according to the physiological capacitance of the plant leaf; further calculating physiological inductive reactance of the plant leaf; adopting a physiological resistance reciprocal of the plant leaf as a reference, and obtaining a relative electric capacity and a relative carrying capacity of a plant leaf cell; further acquiring a conveying capacity of the plant leaf cell. The method provided by the invention can be used for quickly, losslessly and quantitively detecting the conveying capacities of different plant leaf cells on line, filling the blank of representing a cell conveying capacity by utilizing biological physical indexes, and providing a mode for the quantitation of a cell membrane function.

Description

technical field [0001] The invention belongs to the technical field of agricultural engineering and crop information detection, and specifically relates to a method for measuring the transport capacity of plant leaf cells, which can quickly and non-destructively detect the transport capacity of plant leaf cells, and judge the water metabolism of plant leaf cells and the transport of photosynthetic products ability. Background technique [0002] The cell membrane is mainly composed of lipids (mainly phospholipids) (about 50% of the total cell membrane), proteins (about 40% of the total cell membrane) and sugars (about 2%-10% of the total cell membrane) and other substances. Composition; mainly protein and lipid. The phospholipid bilayer is the basic scaffolding of the cell membrane, see figure 1 . Under the electron microscope, it can be divided into three layers, that is, there is an electron-dense belt (hydrophilic part) with a thickness of about 2.5nm on the inner and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N13/00G01N27/02G01N27/04G01N27/22
CPCG01N13/00G01N27/02G01N27/041G01N27/22
Inventor 吴沿友吴沿胜黎明鸿方蕾饶森苏跃王瑞
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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