Three-dimensional dyeing method for amygdalus communis l anther cells

A technology of almond blossoms and cells, which is applied in the field of three-dimensional staining of almond anther cells, which can solve the problems of not being able to observe various cells well, not being able to grasp the staining time, and complicating the preparation of solutions, etc.

Inactive Publication Date: 2017-05-31
XINJIANG AGRI UNIV
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Problems solved by technology

[0005] It is more complicated to prepare the solution for the traditional Ai’s hematoxylin staining method. Generally, it needs to be cooked for more than 2 months after exposure. The staining time is not suitable, and the staining of cellulose is heavy, and the structural defects of various cells cannot be well observed. , the problem to be solved in the present invention is to provide a kind of ...

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  • Three-dimensional dyeing method for amygdalus communis l anther cells

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Embodiment Construction

[0009] 1 Sample fixation: fix almond flower buds in FAA fixative solution, replace the fixative solution every 3 days, and store the samples in a refrigerator at 4±0.5°C after changing 3 times (the preparation method of FAA fixative solution is 70% absolute ethanol: Glacial acetic acid: 40% formaldehyde solution = 89:5:6). 2 Reagents: 5% hematoxylin absolute ethanol, 30% hydrogen peroxide solution, iodine-potassium iodide solution, 10% ferric chloride solution, 1.5% ferric chloride solution. The preparation method of iodine-potassium iodide solution is to dissolve 2g of potassium iodide into 50ml of hot distilled water, and then add 0.8g of iodine after the potassium iodide is fully dissolved, and it can be used after it is completely dissolved. 3 Dyeing solution formula: mix 5% hematoxylin absolute ethanol, 30% hydrogen peroxide solution, iodine-potassium iodide solution and 10% ferric chloride aqueous solution according to the volume ratio of 10:1:10:15, 10 minutes Finally,...

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Abstract

The invention discloses a three-dimensional dyeing method for amygdalus communis l anther cells. According to the three-dimensional dyeing method, an amygdalus communis l anther slice treated with a conventional paraffin slicing method is dyed with an iron haematoxylin solution prepared 10 min in advance, such that the pollen granulocytes, the tapetal cells, the intermediate cells, the connective cells, the endothecium cells, the epidermic cells, the cells at the connection tissue position and the filaments of the amygdalus communis l anther can be clearly colored, and the coloring degrees among the cells at different parts are different so as to provide the three-dimensional dyeing effect. The three-dimensional dyeing method can be used for the study on the three-dimensional dyeing method internal structure change and the anther development process.

Description

technical field [0001] This patent has invented a three-dimensional dyeing method for almond anther cells. The dye solution prepared 10 minutes in advance is used to dye the almond anther slices processed by the conventional paraffin section method, so that the pollen granule cells and tapetal cells of the almond anther can be dyed , cells in the middle layer, cells at the septum, wall cells in the herb, epidermal cells, cells at the connecting tissue and filaments are clearly colored, and the degree of coloring between cells in different parts is different, with a three-dimensional staining effect, which can be used to study the internal structure changes of almond peach anthers and anther development process. Background technique [0002] Almonds originated in the desert margins and low mountains of Central Asia, and it is generally believed that almonds and peaches have a common ancestor. Mountains formed by crustal movement separated China, Mongolia, and Central Asia mi...

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 李疆李鹏于辉田嘉陆婷罗淑萍
Owner XINJIANG AGRI UNIV
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