Three-dimensional dyeing method for amygdalus communis l anther cells

Abstract

The invention discloses a three-dimensional dyeing method for amygdalus communis l anther cells. According to the three-dimensional dyeing method, an amygdalus communis l anther slice treated with a conventional paraffin slicing method is dyed with an iron haematoxylin solution prepared 10 min in advance, such that the pollen granulocytes, the tapetal cells, the intermediate cells, the connective cells, the endothecium cells, the epidermic cells, the cells at the connection tissue position and the filaments of the amygdalus communis l anther can be clearly colored, and the coloring degrees among the cells at different parts are different so as to provide the three-dimensional dyeing effect. The three-dimensional dyeing method can be used for the study on the three-dimensional dyeing method internal structure change and the anther development process.

Description

technical field [0001] This patent has invented a three-dimensional dyeing method for almond anther cells. The dye solution prepared 10 minutes in advance is used to dye the almond anther slices processed by the conventional paraffin section method, so that the pollen granule cells and tapetal cells of the almond anther can be dyed , cells in the middle layer, cells at the septum, wall cells in the herb, epidermal cells, cells at the connecting tissue and filaments are clearly colored, and the degree of coloring between cells in different parts is different, with a three-dimensional staining effect, which can be used to study the internal structure changes of almond peach anthers and anther development process. Background technique [0002] Almonds originated in the desert margins and low mountains of Central Asia, and it is generally believed that almonds and peaches have a common ancestor. Mountains formed by crustal movement separated China, Mongolia, and Central Asia mi...

AI Technical Summary

Problems solved by technology

[0005] It is more complicated to prepare the solution for the traditional Ai’s hematoxylin staining method. Generally, it needs to be cooked for more than 2 months after exposure. The staining time is not suitable, and the staining of cellulose is heavy, and the structural defects of various cells cannot be well observed. , the problem to be solved in the present invention is to provide a kind of easy and simple three-dimensional staining method of anther cells, which can make the pollen grains, tapetum cells, middle cells, herbaceous compartment cells, herb inner wall cells, epidermal cells, junctions of almond peach anthers The cells and filaments in the tissue are clearly colored, and the degree of coloring between cells in different parts is different, with a three-dimensional staining effect

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