65 results about "Taxus cuspidata" patented technology

The invention provides a method for asexually propagating taxus cuspidata, relating to the method for propagating the taxus cuspidate. The invention solves the problems of low rooting rate and weak nursery stock in the existing cutting propagation method for the taxus cuspidate and low survival rate under natural condition when the taxus cuspidata is transplanted for afforestation on a mountain. The method comprises the following steps: ears are picked up in winter of the first year, and is buried by ice and snow; in spring of the second year, cutting is carried out on a disinfected cutting bed, a film is coated on the cutting bed in 2.5 to 3 months with humidity of between 85 and 88 percent at the temperature of between 26 and 29 DEG C and shading degree of between 70 and 75 percent after cutting is completed, and the cutting bet is subject to fertilizer application and sterilization; the coated film is removed, cold-proof water is irrigated before winter; and the bed is changed in spring of the third year, and culture is conducted for two years to obtain the taxus cuspidata for afforestation. The cultured sapling has 100 percent of lignification, 80 to 96 percent of cut rooting rate and 85 to 90 percent of planting percentage, thus the mature period of medical forest is shortened. The method can be used for large-scale afforestation.

The invention relates to a cuttage breeding method of taxus chinensis in northeast, comprising the following steps: selecting a one-year-old branch as a branch for cuttage; concealing the branch at ashade place, and carrying the branch to a destination after the branch is packaged by a plastic bag; retaining 1-2 leaves on the upper end of each nursery stock stalk when shearing a seedling, shearing the parts longer than 3cm of the retained leaves, and also shearing leaves inserted into the stalk positioned in the middle of a seedling bed; disinfecting a land before the cuttage so as to reducerhizoctonia solani kuhn, and carrying out the cuttage from the middle ten days of October to the first ten days of November or from February to March; using a light shielding net of 80 percent to shield light without directly shining with the sun during the daily management of the seedling; controlling the soil water content at 35-45 percent; and paying attention to the water drainage of the seedling in rainy days and intertillage weed control in ordinary days, and preventing and curing diseases of the seedling in time by chemical agents. By adopting the cuttage breeding method, the taxus chinensis in northeast has high survival rate, fast breeding cultivation and high disease resistance.

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the total synthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate in sequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

The invention relates to the Yew genus plant extract and its extraction method and application, wherein the extract is extracted from the stems and leaves of Northeastern yew and comprises taxone diterpenoid, total lignans, total flavone. The extract can be used as the effective composition for anti-cancer drugs.

The invention provides a paclitaxel oral anticancer preparation obtained by nontoxic extraction from a complete stool of Chinese yew and preparation method thereof, which uses Chinese yew in Northeast China as the main raw material, by a ethanol immersion 16-step method and a nontoxic extraction technology, to obtain a 30% paclitaxel crude product, which is used as main raw material of paclitaxel drug wine and paclitaxel oral liquid. Based on drug effect research, the prepared oral anticancer preparation has greatly visble treatment effect on late period caser patients of 20 kinds of different singularity and the late period and anaphase cancer patients which is failure on combined chemotherapy, and the preparation has advantages of good ease pain effect and non-poison.

The invention discloses a DNA bar code primer pair, kit and method for identifying Taxus chinensis species. The kit comprises the following DNA bar code primer pair including an upstream primer Taxus Indel-F(SEQ ID NO.1) and a downstream primer Taxus Indel-R(SEQ ID NO.2). DNA bar codes include the Taxus chinensis DNA bar code, the Taxus chinensis var.mairei DNA bar code, the Taxus cuspidata DNA bar code, and the Taxus baccata DNA bar code, wherein the sequence of the Taxus chinensis DNA bar code is shown as SEQ ID NO.3 to SEQ ID NO.6, the sequence of the Taxus chinensis var.mairei DNA bar code is shown as SEQ ID NO.7 to SEQ ID NO.8, the sequence of the Taxus cuspidata DNA bar code is shown as SEQ ID NO.9, and the sequence of the Taxus baccata DNA bar code is shown as SEQ ID NO.10. The kit and the identifying method are simple, easy to implement and capable of being effectively used for Taxus species identification, protection and related research, and results are accurate and reliable.

The invention relates to a cultivation method for taxus cuspidata in a cold region, in particular to a light supplement cultivation method for promoting fast growth of taxus cuspidata container seedlings. The purpose is to solve the problems that existing taxus cuspidata container seedlings grow too slowly, an existing light supplement method is unobvious in effect, and after light is supplemented, seedling bodies are weak and even die as whole plants. The light supplement cultivation method includes the steps of (1) collecting, (2) seedling planting, (3) light supplement treatment on the seedlings and (4) fertilization treatment on the seedlings. The light supplement cultivation method has the advantages that after being planted, the taxus cuspidata container seedlings can be recovered fast and adapt to the container seedling culture environment; the cultivation method is easy to implement, low in cost and remarkable in effect; the mode of applying fertilizer of small amount many times is adopted and is beneficial for improving the robustness of nursery stocks; the growth rate of the taxus cuspidata container nursery stocks is increased by over 70%. The cultivation method is mainly used for promoting fast growth of the taxus cuspidata container seedlings.

The invention relates to a nucleotide sequence, a molecular probe and a method for identifying a taxus media seedling. The nucleotide sequence for identifying the taxus media seedling is represented as SEQ ID NO.1; upstream MHSF of the nucleotide molecular probe for identifying the taxus media seedling is represented as SEQ ID NO.2, and downstream MHSR is represented as SEQ ID NO.3; and by means of the nucleotide molecular probe MHSF/MHSR, taxus media is identified with a conventional PCR (polymerase chain reaction) method. The sample consumption is small, and the overall operation can be completed only by a small quantity of samples; accuracy and sensitivity are high; MHSF/MHSR is a specific molecular probe for the taxus media, and negative reaction is caused if T. chinensis or T. cuspidata is detected; and the method is simple, the PCR technology is adopted for detection, time is short, and the detection can be finished by half day.

The invention provides a Taxaceae clone of a stable and high-yield Taxol, in which the content of the Taxol can be 0.8-0.12% of the dry weight of the cultured materials, and is 4-6 times of wild Taxus chinensis. The invention also provides culture technique of the clone and a method for producing the clone culture in large scale rapidly. The method combines liquid and solid culture, produces seed through the liquid culture and obtains the culture materials through the solid culture, selects the culture with excellent comprehensive performance as seeds to be reserved for the liquid culture, and does the process repeatedly. The method can be used for large scale production, the production cycle is shorten greatly compared with the wild type, and has stable quality and high yield. Additionally, the culture process of the method doses not occupy cultivated land, does not damage the wild resource of the Taxus chinensis, protects the environment and solves the problem of shortage of Saussurea involucrate resource.

The invention relates to an open type simplified culture medium for taxus cuspidate. The culture medium comprises a primary culture medium and a subculture medium. The primary culture medium is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, active carbon AC and agricultural streptomycin into optimized B5 basic mother liquor. The subculture is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, 6-benzyl amino adenine BA, active carbon AC and agricultural streptomycin into optimized MS basic mother liquor. According to the culture medium provided by the invention, with the adoption of open type simplified tissue culture, the cost and the inoculating time of the culture medium are remarkably saved, the autoclaved sterilization program is cancelled, and the production flow is simplified by replacing autoclaved sterilization and strict enclosed environment operation by a bacteriostatic agent, so that the labor and equipment investment in industrialized seedling production can be reduced, and the fund is saved by 30-50%.

The invention relates to a method for extracting and purifying a functional element from a traditional Chinese herb and aims at providing a convenient, safe, economical and efficient method for extracting and purifying 7-xylose-10-deacetyl paclitaxel which is a monomeric compound from taxus cuspidata. The technical proposal adopted by the invention comprises the steps: fresh or dried leaves of taxus cuspidate are taken as raw materials, and macroporous adsorption resin enrichment technology, normal-pressure chromatography preparation technology and high-pressure liquid phase separation technology are combined to prepare the 7-xylose-10-deacetyl paclitaxel with the purity higher than 99.90 percent at an extraction rate of 0.05 percent. The raw materials used in the invention can be waste materials from the paclitaxel production, thus fully utilizing natural resources. Moreover, the method comprises extraction, primary impurity removal and repurification, thus being simple and easy; and the loss of the target compound is low.

The invention discloses composite essential oil prepared from taxus cuspidata and coniferous plants and application of the composite essential oil. The composite essential oil prepared from taxus cuspidata and coniferous plants is prepared from taxus cuspidata and coniferous plants at the weight ratio of 1:1-1:3, and the composite essential oil can be used for preparing aroma essential oil, aroma candles and air fresheners for improving indoor air quality or preparing skin essential oil soap. The composite essential oil can effectively purify air, improve air quality and effectively reduce indoor methanal, benzene, methylbenzene, dimethylbenzene, total volatile organic compounds and ammonia content.

The invention discloses an SNP (single nucleotide polymorphism) molecular marker for identifying taxus cuspidata. The SNP molecular marker is positioned at a 444th-site basic group of a sequence shownas SEQ ID NO.1. The taxus cuspidata is identified if the 444th-site basic group is T. The SNP molecular marker has the advantages that systems for quickly identifying the taxus cuspidata can be quickly established on the basis of the SNP molecular marker, accordingly, the purpose of quickly and accurately detecting the taxus cuspidata can be achieved, and the SNP molecular marker has important significance in port species inspection work and the like.

The invention relates to a northeast Chinese yew transplanting method in a coast saline-alkaline region. The method comprises the steps of 1) selecting a place that has a shade and a high physical feature where hydrocele is difficult from the middle ten days of March to the middle ten days of April; 2) digging a tree aperture according to the soil ball size of a transplanted nursery stock; 3) mixing well raw soil dug from the tree aperture with turfy soil and river sand in volume ratio of 1:1:1 to obtain plantation soil; 4) filling part backfilling soil, putting the soil ball of the transplanted nursery stock to guarantee that the rhizome junction of the plant is 10cm higher than the ground, then filling the backfilling soil, stamping and compressing; 5) using an aqueous solution that containing 0.3mmp Naphthalene acetic acid, 0.5ppm indole butyric acid and 300ppm calcium nitrate as a root water. <{EN2}>According to the invention, the Chinese yew can be possibly planted in a coast saline-alkaline region, which means a lot for lifting city greening level and landscape effect.

A consumption habit with flue-cured tobacco cigarettes as a main part is formed in long time in our country, cut tobacco of this kind of flue-cured tobacco cigarettes has is rich in tar and is high in fragrance concentration, smell of cigarettes is thin and insufficient in fragrance due to the fact that the tar content of the cigarettes is sharply lowered, and consumers are difficult to accept it. In the method, Taxus cuspidate plant micropore particles or Taxus cuspidate plant ultra-micropore compound powder are added into cigarette filter tow in proportion, natural states and components of plant components such as Taxus cuspidate, vetiver roots, Houttuynia cordata, couchgrass root, and Reed Rhizome are fully utilized, these plants play a role in a positive effect of adjusting the flavor of the cigarettes, the smoke quality of the cigarettes is improved, harmful gas in smoke can be filtered out, and the tar and nicotine content of main smoke of the cigarettes can be reduced by 5.0-10.0%; and the Taxus cuspidate plant compound particles are added in a large proportion, and then the comprehensive use rate of tobacco raw materials can be greatly improved, and the tobacco leaves consumption can be effectively reduced.

The invention discloses a batch transplanting method for taxus cuspidata seedlings, relates to a transplanting method for taxus chinenesis seedlings in cold regions and belongs to the field of taxus cuspidata seedling transplanting. The technical problems that in the prior art, the taxus cuspidata seedlings are low in survival rate of transplanting and poor in growth in the field are solved. The method includes the steps that 1, chlorophyll fluorescence parameters of transplanted seedling quality are estimated; 2, the environmental suitability of seedling transplanting land is investigated and evaluated; 3, the seedlings are transplanted. By the adoption of the experimental method, after three months of transplanting the taxus cuspidata seedlings, the survival rate is 95%, after five months of transplanting, the survival rate is 90%, and compared with other methods, the survival rate of transplanting is increased by 30-50%.

The invention discloses a tissue culture method of taxus cuspidata. The method includes the following steps that 1, intermediate cells of a newborn branch of taxus cuspidata are selected and treated into a 2 cm tissue slice, the tissue slice is disinfected and then cultured for 15-20 days in a B5 culture medium to form 10-12 cm tissue, and the 5-6 cm part in the middle is selected for the next step of culture; 2, the callus is transplanted into a rooting culture medium for rooting culture, a dark environment is kept in the first three days, and the illumination time of 12-14 h/d is kept three days later till 3 cm main roots emerge. According to the tissue culture method of taxus cuspidata, the intermediate cells of the newborn branch of taxus cuspidata are selected, contain rich taxol and have high proliferation capability, so that the tissue culture period of taxus cuspidata is short, and the survival rate is high; the selected culture medium is simple, easy to obtain and suitable for large-scale culture of taxus cuspidata seedlings.

The invention relates to a low-smoke, halogen-free and flame-retardant thermoplastic elastomer cable material with an ultralow smoke amount. An ethylene-vinyl acetate copolymer, a polyolefin elastomer, an EPDM (ethylene-propylene-diene monomer), hydrogenated base oil, siloxane and a flame retardant are added to a high-speed mixer, organic silicon resin, sodium silicate, maleic anhydride grafted polyolefin elastomers, diammonium hydrogen phosphate, bark of taxus, pine bark, taxus cuspidate, chitosan, a citrus extract, a fresh ginger extract, chlorinated polyethylene resin, polytetrafluoroethylene and polyimide are added and put in an internal mixer, all components are mixed uniformly, and soft jelly is formed; the soft jelly is put in a single screw for mixing, plasticization and melt extrusion, water and volatile substances are extracted, cooling, granulation and drying are performed, and the cable material can be obtained. The low-smoke, halogen-free and flame-retardant cable material with the ultralow smoke amount is produced and releases less smoke during large-area burning.

The invention belongs to the technical field of medicines and particularly relates to a dihydrochlorothiazide (DCT) containing pharmaceutical composition for treating hypertension and a preparation method thereof. The DCT containing pharmaceutical composition for treating the hypertension, provided by the invention, is prepared from the following raw pharmaceutical materials in parts by weight: 15-30 parts of DCT, 2,000-4,000 parts of Eucommia ulmoides, 2,000-4,000 parts of Taxus cuspidata, 2,000-4,000 parts of Pogonatherum paniceum and 2,000-4,000 parts of Poa pratensis. According to the DCT containing pharmaceutical composition for treating the hypertension and the preparation method thereof, the traditional Chinese medicines and the DCT are combined, so that common adverse reactions of the DCT can be avoided, and the treatment effect on the hypertension is improved.

The invention discloses a fertilization method of taxus cuspidata container seedlings and relates to a fertilization method of taxus cuspidata container seedlings in cold regions. The fertilization method of the taxus cuspidata container seedlings solves the technical problems that by means of an existing fertilization method, nutrient leaching of taxus cuspidata seedlings, weak growing vigor of seedlings and withered and yellow needle leaves are prone to being caused as the nutrient utilization efficiency is low. The fertilization method comprises the first step of collection, the second step of planting of seedlings and the third step of fertilization treatment of seedlings. The method has the advantages that operation is easy, cost is low, and the effect is obvious on the aspect of improving the nutrient absorbing efficiency of seedlings; seedlings are recovered rapidly and adapt to the fertilization operation rapidly after taxus cuspidata seedlings are planted; the nutrient absorbing efficiency is as high as 80% and is improved by nearly two times compared with a traditional fertilization method. The method is mainly used for cultivation of taxus cuspidata container seedlings.

The invention provides a method of returning Taxus cuspidata to the wild. The method comprises the steps of S1, selecting seedlings, to be specific, selecting return seedlings 1-2 years or 4-5 years old; S2, lifting the seedlings, to be specific, lifting the return seedlings screened in the step S1, wherein the return seedlings 1-2 years old are lifted by means of nutrient bagging, and the returnseedlings 4-5 years old are lifted by means of bared root lifting; S3, transporting, to be specific, transporting the return seedlings lifted in the step S2 to a forest farm; S4, transplanting, to bespecific, transplanting the return seedlings of step S3 in May; and S5, providing protection, to be specific, applying a plant anti-biting agent to trunks of the return seedlings transplanted in the step S4. The method herein allows Taxus cuspidata to be returned to the wild; seedlings of Taxus cuspidata in nursery are returned to the natural growth environment, and survival rate of Taxus cuspidata to the wild returned to the wild can be increased.

The invention relates to the technical field of low-temperature fresh keeping, in particular to a meat fresh keeping agent prepared from a taxus cuspidata extract. The meat fresh keeping agent comprises the following components in parts by mass of 15-20 parts of sodium hexametaphosphate, 7-10 parts of chitosan, 1-3 parts of tea polyphenols, 1-1.5 parts of lysozyme, 1-2 parts of nisin and 3-5 partsof a taxus cuspidata extract, wherein the taxus cuspidata extract is subjected to dissolving for many times, extraction and purification to obtain extractum; and the total yield of active substancesin the extractum of the taxus cuspidata extract is 2-4%. Lysozymes in the raw materials are egg white lysozymes, and the activity is 25000-30000U/mg. The fresh keeping agent is good in fresh keeping effects, is safe and harmless to human bodies, has certain health-care effects, and is healthy.