The invention discloses an
airlift bioreactor for amplification culture of
taxus cuspidata stem cells. The
airlift bioreactor comprises a tank body, a rotatable spiral guide cylinder, a microbubble generator, a
temperature control device, an air supply device, a material supplementing device, a
pH monitoring and adjusting device, a sampling device and a controller, the microbubble generator is arranged in the middle of the bottom of the tank body and communicated with the
gas supply device; the rotatable spiral guide cylinder is fixed above the microbubble generator and comprises a spiral baffle and a guide cylinder, the guide cylinder is fixed in the center of the interior of the tank body, the spiral baffle is located in the guide cylinder, and the upper end and the lower end of the spiral baffle are rotationally and hermetically connected with the top of the tank body and the microbubble generator respectively; the controller is in
signal control connection with the
temperature control device, the
gas supply device, the material supplementing device, the
pH monitoring and adjusting device and the sampling device. Through a non-mechanically driven dynamic mixing mode, the
bottleneck of
oxygen transfer and
mass transfer in a high-
viscosity culture
system is solved, and efficient reaction equipment is provided for large-scale culture of
plant cells.