DNA bar code primer pair, kit and method for identifying Taxus chinensis species

A barcode primer and barcode technology, applied in the field of biology, can solve the problems of unapplied patent protection and the inability to cluster the same species into one group, and achieve the effects of high accuracy, reliable results and simple experimental operation.

Inactive Publication Date: 2015-12-23
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the single loci TrnL-F and ITS, when they identify the southern yew, there are rich genetic variations within the species, and the same species cannot be completely grouped together, that is to say, these two loci have not yet To achieve the purpose of completely identifying the species of Taxus chinensis
At present, these barcodes and sequences are published in the form of papers and have not applied for patent protection

Method used

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  • DNA bar code primer pair, kit and method for identifying Taxus chinensis species
  • DNA bar code primer pair, kit and method for identifying Taxus chinensis species
  • DNA bar code primer pair, kit and method for identifying Taxus chinensis species

Examples

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Embodiment 1

[0051] Example 1 (primer pair and DNA barcode used in the embodiment are primer pair and DNA barcode described above)

[0052] From different groups, the botanical garden collected 26 specimens of Chinese yew, southern yew, northeast yew and European yew, including 6 Chinese yew specimens and 4 from Hunan Shunhuang Mountain (Tzh1, Tzh3, Tzh4-Tzh5) , 2 from Henan (Tzh2, Tzh6); 12 specimens of Taxus chinensis, 3 from Hunan Shuangpai (Spg6-Spg8), 2 from Hunan Anhua (ah2, ah4), 3 from Hunan Mangshan (ms1-ms2 , ms13), Guangdong Lianshan 2 (ls16, ls20), Guangdong Lechang 1 (cq4), Jiangxi Fenyi 1 (jx6); Northeast yew 6, collected from Japan Forest Research Institute (Tc1-Tc6 ); 2 parts of European yew, collected from Japan Forest Research Institute (Tb1-Tb2).

[0053] These species were identified using the invented DNA barcodes.

[0054] The specific method is as follows:

[0055] 1. The genomic DNA of the other species was extracted according to the improved CTAB method;

[005...

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Abstract

The invention discloses a DNA bar code primer pair, kit and method for identifying Taxus chinensis species. The kit comprises the following DNA bar code primer pair including an upstream primer Taxus Indel-F(SEQ ID NO.1) and a downstream primer Taxus Indel-R(SEQ ID NO.2). DNA bar codes include the Taxus chinensis DNA bar code, the Taxus chinensis var.mairei DNA bar code, the Taxus cuspidata DNA bar code, and the Taxus baccata DNA bar code, wherein the sequence of the Taxus chinensis DNA bar code is shown as SEQ ID NO.3 to SEQ ID NO.6, the sequence of the Taxus chinensis var.mairei DNA bar code is shown as SEQ ID NO.7 to SEQ ID NO.8, the sequence of the Taxus cuspidata DNA bar code is shown as SEQ ID NO.9, and the sequence of the Taxus baccata DNA bar code is shown as SEQ ID NO.10. The kit and the identifying method are simple, easy to implement and capable of being effectively used for Taxus species identification, protection and related research, and results are accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of biology, uses short DNA fragments to identify and identify species, that is, DNA barcoding (DNA barcoding) technology, and specifically relates to a pair of DNA barcoding primers, a kit and a method for identifying yew species. Background technique [0002] DNA barcoding technology is a hotspot in the current international biodiversity research, which uses short standard DNA fragments to quickly and accurately identify and identify species. The Consortium for the Barcode of Life (CBOL) expounded the advantages of DNA barcodes: (1) not limited by individual morphological characteristics; (2) not affected by individual developmental stages; (3) for taxonomic groups that are difficult to distinguish, DNA barcodes are used It can put aside the illusion of morphological similarity and provide classification basis at the gene level; (4) The database composed of nucleotide sequences can provide clear information...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 文亚峰韩文军周宏谢伟东徐刚标
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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