Extraction method of taxol from branches and leaves of artificially cultivated yew

A technique for artificial cultivation of branches and leaves of Taxus chinensis, which is applied in the field of biochemical product preparation, can solve the problems of pollution, difficulty in separation, and high toxicity of the eluent, and achieves the effects of simple process, protection of plant resources and low cost.

Inactive Publication Date: 2011-07-06
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of the separation process are cumbersome, and the yield of paclitaxel with a purity of more than 99% is low
Solvents, eluents, etc. are highly toxic, causing serious pollution
In addition, paclitaxel has similar properties to cephalomannine, 7-epi-paclitaxel and 7-epi-10-deacetyl paclitaxel, making it difficult to separate (Yang et al., J. Chromatogr. A, 1998, 813, 201)

Method used

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  • Extraction method of taxol from branches and leaves of artificially cultivated yew
  • Extraction method of taxol from branches and leaves of artificially cultivated yew
  • Extraction method of taxol from branches and leaves of artificially cultivated yew

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Such as figure 1 As shown, the method of extracting paclitaxel from artificially cultivated yew branches and leaves is:

[0048] (1) Take 1kg of artificially cultivated dried branches and leaves of Taxus Media, crush them with a crusher, add 3L of 90% methanol in a 5L round bottom flask, stir well, and soak them at 40~50℃ three times, each time 4~6 hour. Stir for 3 to 6 times during the placing process. After the soaking is completed, filter to remove waste residue, and concentrate the filtrate to a light green viscous liquid with a volume of about 50ml. Add 2L of petroleum ether to the solution, mix well, add 2L of water, stir for 30min, and leave for about 25min to form the upper light green clear liquid and the lower dark green gelatinous liquid. Separate the upper petroleum ether phase and extract 3 times continuously to remove the fat Impurities such as soluble pigments. Add 2L of propyl acetate to the separated lower aqueous phase, stir for 30 min, leave for about ...

Embodiment 2

[0056] (1) Take 100kg of fresh branches and leaves of artificially cultivated 3-year-old Medica yew, crush them with a crusher and place them in 2m 3 Then add 550L of 90% methanol to the extraction tank, stir evenly, and soak for 4 hours with intermittent stirring at 40-50°C. After the soaking is completed, let out the solution in the extraction tank, and then soak 3 times in the same way. The released methanol solutions are combined and concentrated in a concentration tank to a volume of about 25L. In the extraction tank, add about 250L of petroleum ether to the solution, mix well, add 250L of water and stir for 1 hour, leave it for about 30min to form two layers, and separate the lower layer. Add about 250L of propyl acetate to the lower layer solution, mix well, stir for 1 hour, and leave it for about 30 minutes to form two layers.

[0057] (2) The lower layer of propyl acetate extract was released and evaporated to dryness under reduced pressure to form 1032 grams of brown p...

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Abstract

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the totalsynthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate insequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

Description

Technical field [0001] The content of the present invention belongs to the technical field of biochemical product preparation, and relates to a method for extracting paclitaxel from artificially cultivated yew branches and leaves. Background technique [0002] Paclitaxel (taxol) is an antitumor drug with complex chemical structure and unique mechanism of action extracted from the bark, branches and leaves of Taxaceae (Taxaceae) plants. The source of natural paclitaxel is extremely limited, and it is mainly derived from yew or yew bark (Wani et al., J. Am. Chem. Soc. 1971, 93, 2325). The yew tree species grows extremely slowly. A century-old tree generally can only peel 25kg of bark. If 1kg of pure paclitaxel is extracted to provide 12,000 cancer patients for use, it is equivalent to the bark of 30,000 yew trees. . In order to obtain paclitaxel, methods such as planting dwarf yew trees, tissue culture, semi-synthetic and fully synthetic paclitaxel have been carried out at home a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D305/14
Inventor 高锦明王俊儒张保健
Owner NORTHWEST A & F UNIV
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