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Green making technology of animal tissue paraffin section

A technology of animal tissue and paraffin section, applied in the preparation of test samples, etc., can solve problems such as health damage, asthma, even cancer, and flammability

Inactive Publication Date: 2011-12-14
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xylene is extremely volatile and will cause great harm to people's health, and can cause operators to suffer from asthma and even cancer
[0007] Disadvantages of methyl benzoate: low toxicity, inhalation, oral or percutaneous absorption are harmful to the body
[0008] Cons of Gasoline: Flammable
[0013] In the invention patent with the patent number 200810136961.X "He-stained xylene-free treatment solution for routine paraffin preparation of biological tissue specimens", a mixture of acetone and ethanol was used when dehydrating, but no acetone was used when it was transparent

Method used

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  • Green making technology of animal tissue paraffin section
  • Green making technology of animal tissue paraffin section
  • Green making technology of animal tissue paraffin section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 : Pathological observation of porcine blue ear disease

[0036] Select the various diseased organ tissue pieces of representative diseased pigs, and place them in the mixed fixative solution at 16:00 on the same day.

[0037] At 8 o'clock in the morning of the next day, the tissues were immersed in 85% ethanol for 1 hour.

[0038] At 9 o'clock, the tissue was soaked in 95% ethanol for 1 hour.

[0039] At 10 o'clock, tissue into 95% ethanol for 1 hour

[0040] At 11:00, put the tissue into absolute ethanol I for 1 hour

[0041] At 12 o'clock, put the tissue into absolute ethanol II for 1 hour (if using a 60°C oven, the above dehydration time is 40 minutes)

[0042] At 13 o'clock, put the tissue into the mixed transparent agent for 3 hours (if using a 60°C oven, the time is 2 hours)

[0043] At 16:00, place the tissue in paraffin I with a melting point of 52-54°C for 1 hour (in a 60°C oven)

[0044] At 17:00, put the tissue into paraffin II with a melting ...

Embodiment 2

[0058] Example 2 : Pathological observation of rex rabbit mite disease

[0059] Get skin lesion tissue, carry out by the method for tissue wax block making and section staining of the pathological observation of embodiment 1 porcine PRRS. The following are the histopathological observations of this case.

[0060] Thickening of the keratinized layer of the skin, telangiectasia and hyperemia, thickening of the keratinized layer, loosening and edema of the subcutaneous tissue (inflammation), a large number of parasites, and infiltration of inflammatory cells.

Embodiment 3

[0061] Example 3 : Pathological observation of trichomoniasis in turkey

[0062] Get sick dead chicken liver lesion lesion tissue, carry out according to the method for the tissue wax block making of the pathological observation of pig blue ear disease of embodiment 1 and section staining. The following are the histopathological observations of this case.

[0063] There were scattered and extensive confluent hepatic necrosis in the parenchyma of the turkey liver, and the boundary with the surrounding tissues was unclear. Tissue loss in the liver. Interlobular bile duct dilation, bile duct trichomonas parasitism. The number of bile ducts increased, and the epithelial cells of the bile ducts were shed.

[0064] In summary, the present invention is a technology for making animal tissue paraffin sections by green film-making method, and its characteristics are as follows:

[0065] 1. From the whole process of wax block making and section staining, there are few kinds of reag...

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Abstract

A green making technology of animal tissue paraffin section disclosed in the invention aims at providing a technique of making a paraffin section of animal tissue applied to the agricultural university medicine and other majors, characterized by that the type of chemical reagents that are used is limited, the chemical reagents have no toxicity, and dehydrate the tissue thoroughly, accelerate making sections but not lead to excessive contraction and increasement of hardness of the tissue, and substitute dimethyl benzene used in the traditional making technology of the paraffin section which isharmful to human body, so as to eliminate the occupational diseases and environmental pollution caused by dimethyl benzene. The making technology comprises the following steps: carrying out fixation with a fixative solution (100ml of the fixative solution comprises 91 ml 85% alcohol, 4 ml methanol and 5 ml glacial acetic acid ), dehydrating the tissue with graded ethanol, then putting the fixative and dehydrated tissue in a mixed transparent reagent (comprising 14wt% of analytically pour n-butanol, 29wt% of acetone and 57wt% of absolute alcohol ) for standing for 3 hours, then carrying out normal waxing and embedding, using analytically pour turpentine (60 DEG C) for section staining, carrying out dewaxing twice, carrying out hydration under normal temperature, using haematoxylin for staining nuclei, decoloration and bluing, and using eosin for staining, dehydrating until get absolute alcohol, then carrying out air drying, and directly using the gum diluted by turpentine to sealing the section.

Description

technical field [0001] The invention relates to a method for making paraffin sections of animal tissues. Background technique [0002] Paraffin section technology is the main experimental method of histology and developmental biology research, and it is also an important means of observing pathological changes in pathology. It is widely used in clinical pathological diagnosis, teaching and scientific research. Morphological observation of animal specimens, immunohistochemistry, in situ hybridization and in situ PCR and other technical means are inseparable from the processing of specimen tissues and the preparation of paraffin sections. [0003] Conventional tissue paraffin sectioning techniques use xylene as a clearing agent and dewaxing agent, which has a strong shrinkage and quick effect on the tissue, but the tissue should not be kept in xylene for too long, otherwise the tissue will easily become brittle and hard. Xylene is extremely volatile and will cause great harm ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
Inventor 范春玲周玉龙任亚超郭婷齐欣兰明慧孙斌郭东华吴海燕
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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