Method for staining medical tissue slice

Abstract

The invention provides a medical tissue section staining method which comprises the following steps that: olefin is sliced into sheets, dewaxed and hydrated; sliced sheets are put into solution of picric acid, formaldehyde and glacial acetic acid after being heated, taken out and washed by running water; the sliced sheets are put into a 5 percent sodium thiosulfate solution to soak and washed by distilled water; the sliced sheets are put into an alcian blue solution to soak and washed by the running water; the sliced sheets are put into a preheated alkaline ethanol solution to soak and washed by the running water; under the lighttight condition, the sliced sheets are put into a haematoxylin working solution and washed by the running water and the distilled water; the sliced sheets are put into a saffron/ acid fuchsine working solution and washed by the distilled water; the sliced sheets are put into a phosphotungstic acid solution to soaked, then transferred to the glacial acetic acid to soak and washed by the distilled water; the sliced sheets are dehydrated by ethanol; the sliced sheets are put into an ethanol saffron solution to soak, then dehydrated by the ethanol and sealed. The medical tissue section staining method has low cost and vivid staining, shortens the time for a staining flow and also increases the application range.

Description

technical field [0001] The invention relates to a medical tissue section staining method for observing pathological changes of diseases by staining the tissue sections in medical research. Background technique [0002] In medical research, it is extremely important to observe the pathological changes of diseases through tissue sections. The commonly used HE (hematoxylin-eosin) staining method can only display blue nuclei and red cytoplasm, and immunohistochemical staining can only specifically display one cell component on a slice. There are certain restrictions on clearly distinguishing the various tissue components. The Movat (Movat) overstaining method of the Department of Pathology, University of Washington in the United States can display collagen fibers, proteoglycans, muscle fibers, elastic fibers, fibrin, etc. on a tissue section with five different colors, and the staining effect is good. , but the cost of the dyes used (saffron, Alcian blue, acid fuchsin, scarlet...

AI Technical Summary

Problems solved by technology

There are certain restrictions on clearly distinguishing the various tissue components

The Movat (Movat) overstaining method of the Department of Pathology, University of Washington in the United States can display collagen fibers, proteoglycans, muscle fibers, elastic fibers, fibrin, etc. on a tissue section with five different colors, and the staining effect is good. , but the cost of the dyes used (saffron, Alcian blue, acid fuchsin, scarlet, hematoxylin, picric acid, iodine, etc.) is too high, and it is difficult for general laboratories to bear. In addition, the dyeing procedure of this method requires 8 hours too long

And the Movat overstaining method is limited to the paraffin section staining of the atherosclerotic vessel wall