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Zooplankton microscopic slide specimen preparation method

A technology for making microscopic slides and specimens, which is applied in the preparation of test samples, etc., can solve the problems of material damage, unfavorable long-term storage of materials, unfavorable body organ tissue shape and structure, etc., and achieve the effect of improving clarity and contrast

Inactive Publication Date: 2014-09-17
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are generally two types of zooplankton specimens used for the overall observation of the microscope. One is a liquid-based specimen that is fixed and preserved with a fixative. Put it back into the specimen bottle or throw it away. In the process, it is easy to cause damage to the material, which is not conducive to the long-term preservation of the material
The other is dry specimens, which generally use ordinary glass slides as the carrier, and the materials are made through the steps of fixing, drying, and sealing through smearing, pressing, and overall mounting. Due to the smaller size of zooplankton, and sometimes cause damage to the structure of the organism, it is not conducive to truly reflecting the shape and structure of the body's organs and tissues

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0016] The detailed operation steps of the above-mentioned zooplankton microscope slide specimen preparation technology are as follows:

[0017] 1) Take zooplankton material and fix it with 5% formalin for 2-3 hours;

[0018] 2) Stain the fixed zooplankton material with hematoxylin for 20-30 minutes;

[0019] 3) Separate the dyed zooplankton material with acid alcohol for 3 to 5 minutes;

[0020] 4) Blue the lecherous zooplankton material with tap water for 20-30 minutes;

[0021] 5) Dehydrate the blanched zooplankton material through distilled water, 50% alcohol, 70% alcohol, 80% alcohol, 95% alcohol and two-step anhydrous alcohol in sequence, and each level of medicine lasts about 5 minutes;

[0022] 6) The dehydrated zooplankton material is made transparent through a mixture of glycerin and absolute alcohol with a volume ratio of 1:1, and two steps of pure glycerin in sequence. Each level of medicine lasts about 5 minutes;

[0023] 7) Absorb the above-mentioned transpare...

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Abstract

The invention provides a zooplankton microscopic slide specimen preparation technology. The zooplankton microscopic slide specimen preparation method comprises the following steps: with a concave slide as a carrier, carrying out formalin fixation, haematoxylin dyeing, acid alcohol color separation, tap water bluing, alcohol dewatering, glycerin hyalinizing, and neutral balsam sealing, thereby preparing a zooplankton microscopic slide specimen. The long-time storage of zooplankton materials is realized, the morphological structures of the materials can be kept maximally, and the observation clarity and contrast of the specimen can be improved.

Description

technical field [0001] The invention relates to a technology for making microscopic slide specimens of zooplankton, and relates to the field of making microscopic specimens. Background technique [0002] In the field of biological sciences, it is essential to observe zooplankton as a whole with a microscope. At present, there are generally two types of zooplankton specimens used for the overall observation of the microscope. One is a liquid-based specimen that is fixed and preserved with a fixative. Put it back into the specimen bottle or throw it away. In the process, it is easy to cause damage to the material, which is not conducive to the long-term preservation of the material. The other is dry specimens, which generally use ordinary glass slides as the carrier, and the materials are made through the steps of fixing, drying, and sealing through smearing, pressing, and overall mounting. Due to the small size of zooplankton, and sometimes cause damage to the structure of ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 郭恩棉任巧萌孙振华张发承
Owner QINGDAO AGRI UNIV
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