Rapid cast-off cell sample dyeing agent and preparation method thereof
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A technique of exfoliating cells and rapid staining, applied in the preparation of test samples, organic dyes, etc., can solve the problems of delayed patient examination time, large economic burden on patients, and high labor costs, and achieves early clinical diagnosis time, and the method is simple and easy. The effect of mastering, low cost of material consumption
Inactive Publication Date: 2015-09-09
运城市妇幼保健院
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Due to the complex staining procedure, many steps (13-14 steps) and long time-consuming (about 1 hour) in the existing technology, microscopic examination cannot be performed immediately, and it takes a long time to provide a clinical diagnosis report,
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[0012] The present invention is further illustrated below by way of examples.
[0013] A rapid staining agent for exfoliated cell specimens, comprising the following components by weight percentage: 3.5 grams of hematoxylin, 45 grams of absolute ethanol, 50 grams of potassium vanadium, 500 grams of distilled water, 2 grams of mercury oxide, and 6 grams of glacial acetic acid.
[0014] The preparation method of the above-mentioned fast staining agent for exfoliated cell specimens is as follows: first, put potassium vanadium into distilled water and heat it to about 80 degrees to dissolve it, and cool to normal temperature to obtain a solution of potassium vanadium; add hematoxylin into absolute ethanol After dissolving, it is mixed with potassium vanadium dissolving solution, and then mercuric oxide and glacial acetic acid are added in sequence to prepare a rapid staining agent for exfoliated cell specimens.
[0015] Using method of the present invention: 1, the exfoliated cell...
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Abstract
The invention relates to a rapid cast-off cell sample dyeing agent and a preparation method thereof. The rapid cast-off cell sample dyeing agent comprises the following components in parts by weight: 3.5 parts of haematoxylin, 45 parts of absolute ethyl alcohol, 50 parts of aluminum potassium sulfate dodecahydrate, 500 parts of distilled water, 2 parts of mercuric oxide and 6 parts of glacial acetic acid. The preparation method of the rapid cast-off cell sample dyeing agent comprises the following steps: firstly putting aluminum potassium sulfate dodecahydrate into distilled water, heating to about 80 DEG C for dissolving aluminum potassium sulfate dodecahydrate, and cooling to normal temperature, so that an aluminum potassium sulfate dodecahydrate dissolved solution is obtained; and adding haematoxylin into absolute ethyl alcohol, dissolving, mixing with the aluminum potassium sulfate dodecahydrate dissolved solution, and then sequentially adding mercuric oxide and glacial acetic acid, so that the rapid cast-off cell sample dyeing agent is obtained. A cast-off cell smear sample is obtained by dyeing for 30+/-s by utilizing the rapid cast-off cell sample dyeing agent and washing for 10+/-s with flowing water. Compared with the prior art, the rapid cast-off cell sample dyeing agent has the beneficial effects that the dyeing time is shortened to 1 minute from 1 hour, the clinical diagnosis time is much earlier, a method is simple and easy to learn, and the cost of used raw materials is low; meanwhile, the time consumed by inspection personnel is less, the labour cost is low, and the financial burden of a patient is low.
Description
technical field [0001] The invention relates to a staining agent for microscopic examination specimens in the pathological diagnosis of cells in the medical field and a preparation method thereof, in particular to a rapid staining agent for exfoliated cell specimens and a preparation method thereof. Background technique [0002] At present, for the pathological diagnosis of exfoliated cells, hematoxylin-eosin staining (HE staining) or Papanicolaou staining is routinely used in cell smears; such as HE staining steps: 1, smear fixation 10 ± min, 2. Washing with water for 2-3 minutes, 3. Hematoxylin solution for 2 minutes, 4. Washing with tap water for 2-3 times, 5. 0.5% hydrochloric acid aqueous solution for 2-3s, 6. Rinse with tap water for 2-3 times, 7. Lithium carbonate solution 1min, 8, 95% ethanol I, II each 1min, 9, eosin solution 1min, 10, 95% ethanol I, II, III, IV each 30s, 11, absolute ethanol I, II each 30s, 12, xylene I, II each 30s, 13, neutral gum sealing. Such...
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