Preparation method of hyriopsis cumingii pallium tissue slice

A technique for slicing of Triangula ceruleus and tissue, applied in the biological field, can solve problems such as blurred cell images, and achieve the effects of simple operation, reduced error probability, and easy preservation

Inactive Publication Date: 2013-08-21
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent document CN201010585906.6, with an application date of December 13, 2010, discloses a method for fast slicing fish tissue, including steps such as fixing, slicing, patching, staining and sealing of fish tissue, which can effectively sol

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  • Preparation method of hyriopsis cumingii pallium tissue slice
  • Preparation method of hyriopsis cumingii pallium tissue slice
  • Preparation method of hyriopsis cumingii pallium tissue slice

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Embodiment 1

[0034] The tissue section provided by the present invention is used to observe the position and structure of the mantle tissue cells of bivalves. On the basis of anatomical preliminary positioning, the mantle tissue of the triangular mussel is clipped along the shell edge and fixed, dehydrated, transparent, dipped in wax, After embedding, slicing, patching and drying, and finally staining with hematoxylin-eosin, serial slices of the mantle tissue were made, and the positional relationship and structural characteristics of the tissue cells in each layer of the mantle were observed under a biological microscope to obtain A clear and complete atlas of mantle tissue sections.

[0035] Concretely take the triangle sail as an example, and describe the method content of this invention in detail.

[0036] 1. Selection of Triangle sailfish and collection of mantle tissue

[0037] Select healthy 3-year-old triangular sail mussels (shell length > 10 cm, shell width > 8 cm), the shells a...

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Abstract

The invention discloses a preparation method of a hyriopsis cumingii pallium tissue slice. The preparation method comprises the following steps of: carrying out fixation, upstream gradient ethanol dehydration, vifrification, waxdip, embedding, sectioning, baking, dewaxing and downstream gradient ethanol rehydration on pallium tissue on cumingii pallium, and mounting on the slice tissue by adopting neutral resins after carrying out hematoxylin-eosin on the slice tissue. The tissue slice prepared by the invention can be used for observing the histology characteristic of the pallium, and detecting expression of related genes forming the shell on the positions of the hyriopsis cumingii pallium; the method provided by the invention can guarantee the relative stationarity between positions of each layers of tissues of the pallium and the cell; and compared with the common preparation method of the tissue slice, the preparation method provided by the invention has the advantages that time is saved, the problem of tissue fracture of the tissue slice for the pallium manufactured by the common method is overcome, and the repeatability is better.

Description

Technical field [0001] The present invention involves the field of biotechnology. Specifically, it is a preparation method for tissue slices of triangular sail mussels. Background technique [0002] Triangle sailing mussels are the most important high -quality freshwater rackets in my country, and have important economic value.The coat film of Yuzhu mussels can secrete the substance to form a shell, and it can also secrete the pearl quality to form pearls.The mussel shell of freshwater mussels can be divided into three layers of stratum corneum, edges and pearl layers. The different multi -layer structure of the shell is generally considered to be caused by different types of the outer skin cells of the coat, that is, the stratum corneum is consisted of the coat film.The protruding secretion of raw shells is formed, the bondeline layer is formed by the secretion of high -columnar cells of the edge of the coat film, and the pearl layer is formed by the cylindrical cells of the cen...

Claims

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Application Information

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IPC IPC(8): G01N1/06G01N1/30
Inventor 李家乐李西雷白志毅汪桂玲罗红瑞
Owner SHANGHAI OCEAN UNIV
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