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Membrane-based cell-derived xenograft (M-CDX) tumor model-based tissue slice making method

A technology of M-CDX and tissue slicing, which is applied in the preparation of test samples, etc., can solve the problems of high cost and strictness, and achieve the effect of simple operation, short time consumption, and reduced alcohol dehydration time

Pending Publication Date: 2018-12-28
广州星燎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the stricter feeding and breeding requirements of nude mice and the higher price, there are certain limitations in related nude mouse transplantation tumor models.

Method used

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  • Membrane-based cell-derived xenograft (M-CDX) tumor model-based tissue slice making method
  • Membrane-based cell-derived xenograft (M-CDX) tumor model-based tissue slice making method

Examples

Experimental program
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Effect test

Embodiment

[0023] Choose healthy embryonated eggs and incubate them in an incubator with a temperature of 37°C and a humidity of 60% for 7 days. If the embryonic eggs are intact and intact, gently insert the shell opener into the top of the embryonic eggs, and slowly open the double shells. Be careful not to Destruction of the integrity of the ectoembryonic egg. Use scissors to cut a window of 1 cm in length and width along the edge of the shell to the top of the shell;

[0024] Will 1*10 6 A certain amount of tumor cells are incorporated into the above-mentioned extracellular matrix, and obtained by centrifuging at 25°C at 300RCF for 5 minutes;

[0025] Put the clipped chorioallantoic membrane into a sterilized silicone ring and incubate for 3 days in an incubator at 37°C and 60% humidity. Cut off the tissue and its surrounding chorioallantoic membrane with surgical scissors, and carry out Embedded in paraffin, sectioned, and then stained with HE and examined under a microscope.

[0...

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Abstract

The invention relates to a tissue slice making method, in particular to a membrane-based cell-derived xenograft (M-CDX) tumor model-based tissue slice making method. The M-CDX tumor model-based tissueslice making method comprises the steps of incubating an embryonated egg in an incubator with temperature being 36-38 DEG C and humidity being 60-80% for 5-7 days, and cutting a window being 1 centimeter by 1 centimeter in a top end of the embryonated egg; and doping tumor cells in quantity being (0.5-8)*10<6> to an extracellular matrix to prepare a cell suspension liquid, dropwise adding the cell suspension liquid to a silica gel ring placed at a chorioallantoic membrane in advance, incubating the cells for 3 days at 37 DEG C and humidity being 60%, cutting tissue and the chorioallantoic membrane around the tissue by operating scissors, performing paraffin embedment and slicing, and performing HE dying and microscopic examination. The slice tissue prepared by the method comprises a complete cell structure type, each layer of tissue and cell position are relatively fixed, the morphological position relation of each layer of tissue and the cells is conveniently observed, the tissue iseasy to store, the M-CDX tumor model-based tissue slice making method is suitable for rapid and mass preparation of tissue slice, and a good test foundation is provided for researching effects of immunology and oncology during the formation process.

Description

technical field [0001] The invention relates to a method for making tissue slices, in particular to a method for making tissue slices based on an M-CDX tumor formation model. Background technique [0002] Membrane-based cell-derived xenograft (M-CDX) is a membrane-based cell-derived xenograft, which is a model constructed by transplanting tumor cell lines of xenogeneic species into the chicken chorioallantoic membrane. This model can be used for the interaction between angiogenesis and tumor development, as well as for the screening of effective anti-tumor compounds. [0003] Prior art A mouse model of circulating tumor cells, its construction method and application (CN201710364391.9), the method includes the following steps: (1) Subcutaneous transplantation: Transplant primary tumor tissue samples into the body of immunodeficient mice , to construct the primary cancer xenograft model PDX; (2) collection of circulating tumor cells: collection of circulating tumor cells in t...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 文皓旻
Owner 广州星燎生物科技有限公司
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