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Application of ginsenoside Rg3 in combination with GP

A technology of ginsenosides and drugs, which is applied in the field of tumor adjuvant drug development, can solve the problems of high toxicity and achieve the effect of enhancing the inhibitory effect

Inactive Publication Date: 2020-12-11
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an application of ginsenoside Rg3 combined with GP as an anti-tumor drug, aiming to overcome the problem of excessive toxicity of existing GP chemotherapy methods

Method used

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  • Application of ginsenoside Rg3 in combination with GP
  • Application of ginsenoside Rg3 in combination with GP
  • Application of ginsenoside Rg3 in combination with GP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example relates to the research on the effect of ginsenoside Rg3 combined with GP chemotherapy on inhibiting the growth of Lewis lung cancer.

[0027] 1. Experimental materials and methods

[0028] (1) Main materials

[0029] 1. Experimental animals: 32 SPF-grade female C57BL / 6N mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. [SCXK9 (Beijing) 2016-0006], and were kept in separate cages in the Experimental Animal Center of Ningxia Medical University [Ningxia Medical University Ethics No. 2018-50], average body weight (14±2) g. Experimental animals Mice were free to drink water and food, the ambient temperature was (20±2)°C, and the relative humidity was (70±2)%. The study in this example conforms to the 3R principle and has been approved by the Experimental Animal Ethics Committee of Ningxia Medical University. Ethics Number: IACUC-NYLAC-2019-104

[0030] 2. Cell line: Mouse Lewis lung cancer cell line (LLC) was purchased ...

Embodiment 2

[0056] This example relates to the effect of ginsenoside Rg3 combined with GP chemotherapy on liver, kidney, spleen and tumor pathological changes and organ indexes of Lewis lung cancer mice.

[0057] 1. Experimental materials and experimental methods

[0058] 1. Main reagents: Hematoxylin (purchased from Shanghai Yuanye Biotechnology Co., Ltd.); Eosin (purchased from Shanghai Yuanye Biotechnology Co., Ltd.)

[0059] 2. Experimental equipment: upright fluorescence microscope (OLYMPUS); cryostat (Leica, Germany)

[0060] 3. Experimental method

[0061] 3.1. Detection of visceral index: after the treatment, the liver, kidney and spleen were peeled off and weighed to calculate their visceral index.

[0062] 3.2. HE staining method:

[0063] (1) Fixation: 20 mL of 4% paraformic acid was dispensed into vials, and the removed fresh organ tissue was placed in it, and fixed for 48 hours;

[0064] (2) Block trimming: Cut the fixed liver tissue into small pieces of 0.5×0.5 cm, put t...

Embodiment 3

[0090] This example relates to the effect of ginsenoside Rg3 combined with GP chemotherapy on tumor microvessel density and CD62P protein.

[0091] 1. Main reagents: MVD kit was purchased from Beijing Biosen Biotechnology Co., Ltd. (batch number: AH03095376); CD62P kit was purchased from Beijing Biosen Biotechnology Co., Ltd. (lot number: AH11156358).

[0092] 2. Experimental equipment: upright fluorescence microscope (OLYMPUS); cryostat (Leica, Germany)

[0093] 3. Immunohistochemical staining method

[0094] (1) Fixation: 20 mL of 4% paraformic acid was dispensed into vials, and the removed fresh tumor tissue was placed in it, and fixed for 48 hours;

[0095] (2) Block trimming: Cut the fixed liver tissue into small pieces of 0.5×0.5 cm, put them into the embedding frame, and wash them with running water for 30 minutes;

[0096] (3) Dehydration: Dehydration treatment is carried out by a fully automatic dehydration machine: the specimen is placed in 75%, 85%, 90% concentrat...

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Abstract

The invention discloses application of ginsenoside Rg3 in combination with GP as an antitumor drug. A mouse Lewis lung cancer cell line (LLC) is selected and inoculated to the right armpit of a C57BL / 6N mouse, and the influence of ginsenoside Rg3 and Rg3 in combination with GP on Lewis lung cancer is observed; hematoxylin-eosin staining is performed on the liver, kidney, spleen and tumors, and pathological changes of ginsenoside Rg3 in combination with GP on the liver, kidney, spleen and tumors of mice with the Lewis lung cancer are detected; and tumor tissues are subjected to immunohistochemical staining, and the density of blood vessels in tumors and the expression of tumor platelet surface activation markers are detected. The conclusion is that the ginsenoside Rg3 in combination with GPcan enhance the effects of ginsenoside Rg3 of inhibiting the tumor growth of the mice with the Lewis lung cancer and inhibiting tumor angiogenesis, and the tumor inhibition effect of the ginsenosideRg3 is possibly and closely related to CD62P expression reduction; and the ginsenoside Rg3 in combination with GP can achieve the synergistic and toxicity-reducing effects.

Description

technical field [0001] The invention belongs to the technical field of drug development for tumor adjuvant therapy, and in particular relates to an application of ginsenoside Rg3 combined with GP (gemcitabine+cisplatin) as an anti-tumor drug. Background technique [0002] In recent years, the incidence of cancer in my country has been increasing year by year, and the number of deaths caused by it is second only to cardiovascular and cerebrovascular diseases. In particular, the incidence and mortality of lung cancer rank first in my country. Combination chemotherapy based on gemcitabine and cisplatin (GP) is one of the main methods for the treatment of lung cancer, but GP chemotherapy can damage immune cells while killing tumor cells, and is accompanied by some severe liver, kidney and bone marrow suppression. toxicity. Therefore, it is of great clinical significance to explore adjuvant therapy drugs that can prevent and treat tumors and reduce these toxic and side effects t...

Claims

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Application Information

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IPC IPC(8): A61K31/7068A61K33/243A61K31/704A61P35/00
CPCA61K31/704A61K31/7068A61P35/00A61K33/243A61K2300/00
Inventor 汪静王柯
Owner NINGXIA MEDICAL UNIV
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