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Laser microdissection system and examination method for samples containing nucleic acid

A laser microdissection and inspection method technology, applied in chemical instruments and methods, microscopes, laboratory containers, etc., can solve the problems of inability to directly observe samples, image processing and analysis methods are cumbersome and time-consuming, etc.

Active Publication Date: 2016-02-24
LEICA MICROSYSTEMS CMS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this has, inter alia, the disadvantage that during the generation of the cutting line, the sample cannot be viewed straight-forward, as the sample moves within the field of view and the image appears blurred
[0012] However, single cell-gel electrophoresis is usually performed in small quantities and with poor reproducibility
The image processing and analysis methods used are cumbersome, time-consuming and potentially error-prone

Method used

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  • Laser microdissection system and examination method for samples containing nucleic acid
  • Laser microdissection system and examination method for samples containing nucleic acid
  • Laser microdissection system and examination method for samples containing nucleic acid

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Embodiment Construction

[0056] exist figure 1 A laser microdissection system generally indicated at 100 that can be used to practice the method of the present invention is schematically shown in FIG. The main parts of the laser microdissection system 100 substantially correspond to those disclosed in EP1276586B1, to which reference is expressly made here. The coordinate system used to represent the axes or directions x, y and z described below is designated 110 .

[0057] Laser microdissection system 100 includes microscope 10 . On the microscope stand 11 of the microscope 10 , an illumination device 12 , which is only partially shown here, can be arranged. The lighting device can comprise, for example, a (not shown) light source and suitable means for influencing the illumination light supplied by the light source, such as filters and / or light barriers.

[0058] For example, a user input unit and / or a user information unit 13 can also be arranged on the microscope stand 11, which can be configure...

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Abstract

The invention relates to a laser microdissection system (100), comprising a microscope (10). The microscope comprises a reflected-light device (76), a microscope objective (41), and a laser unit (70). A beam path (b) of a laser beam of the laser unit (70) extends through the reflected-light device (76) and through the microscope objective (41) and intersects with an object plane (15) of the microscope objective (41) at an adjustable point of intersection. An electrophoresis unit (90) having an electrophoresis gel (91) is attached, or can be attached by means of coupling elements (35), below the object plane (15), wherein the electrophoresis gel (91) has at least one gel pocket (92) and the laser microdissection system (100) comprises positioning means (96) for positioning the electrophoresis gel (91) in parallel with the object plane (15) and in relation to a defined reference position, such that dissectates (99) of a sample (51) that can be arranged in the object plane (15), which dissectates are obtained by means of the laser beam of the laser unit (70), can be captured in the at least one gel pocket (92). The invention further relates to a corresponding electrophoresis unit (90) and an examination method.

Description

technical field [0001] The invention relates to a laser microdissection system according to the preambles of the independent claims, an electrophoresis unit for such a laser microdissection system and a corresponding examination method for samples containing nucleic acids. Background technique [0002] Methods for processing biological samples by so-called laser microdissection have been known since the mid-1970s (see for example Isenberg, G. et al.: Cell surgery by laser micro-dissection: a preparative method. Journal of Microscopy (Journal of Microscopy, using laser microscopy). Microdissection of Cellular Surgery: A Preliminary Approach), Vol. 107, 1976, pp. 19-24), and has been continuously improved since then. [0003] In laser microdissection, cells, tissue regions etc. can be isolated from a sample ("sample", "specimen") and obtained as so-called sections. A particular advantage of laser microdissection is the brief contact of the sample with the laser beam, from whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N1/28G02B21/32B01L3/00
CPCG01N27/44743G01N27/44782G01N1/286G02B21/32G01N2001/2886G01N2001/284G01N27/44721G02B21/0028G02B21/26C12N15/1003G02B21/0016G01N1/2813G02B21/0048G02B21/18
Inventor 福尔克·施劳德拉夫
Owner LEICA MICROSYSTEMS CMS GMBH
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