The invention relates to a
laser microscope (20), in particular a multiphoton
microscope, for the histological examination of a
tissue sample (14) comprising a
laser light source (27), a
laser objective (21), a measurement
signal acquisition module (28), and a sample holder (30) for receiving a
tissue sample (14) and / or a sample holder for a
tissue sample (14), wherein the
laser light source (27) is configured to generate
laser light L with an
excitation wavelength between 950 nm and 1200 nm, in particular between 1000 nm and 1100 nm, in particular between 1030 nm and 1070 nm, preferably 1050 nm, and the measurement
signal acquisition module (28) is arranged and configured to detect
fluorescence light (XF) reflected from the tissue sample (14) into the laser objective (21), in particular with a
fluorescence wavelength between 500 nm and 700 nm, preferably between 550 nm and 650 nm, and / or
ultraviolet GHG light (XT), especially with a GHG
wavelength between 300 nm and 400 nm,The invention is characterized in that the sample holder (30) is arranged above the laser objective (21) such that
laser light (L) emitted by the laser objective (21) passes through the sample holder (30) and, in particular, only through it, and onto the tissue sample (14), wherein the sample holder (30) is freely accessible on a side opposite the laser objective (21).