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Method for detecting virus content of swine fever live vaccine through indirect immunofluorescence

A technology for immunofluorescence detection and swine fever live vaccine, which is applied in the direction of measuring devices, fluorescence/phosphorescence, instruments, etc., can solve the problems of poor repeatability, time-consuming and laborious, complicated operation, etc., and achieve low cost, accurate detection results, and simple operation Effect

Inactive Publication Date: 2014-04-02
SHANDONG BINZHOU BOLAIWEI BIOTECH
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AI Technical Summary

Problems solved by technology

[0003] At present, the detection method of the virus content of the live swine fever vaccine produced in my country is the rabbit reaction heat method, which has the disadvantages of time-consuming and laborious, complicated operation, high cost, and poor repeatability.

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  • Method for detecting virus content of swine fever live vaccine through indirect immunofluorescence

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Embodiment Construction

[0027] The present invention will be described in detail below through specific examples.

[0028] A kind of method utilizing indirect immunofluorescence detection swine fever live vaccine virus content, comprises the steps:

[0029] (1) Prepare cells for passage

[0030] Digest and disperse the monolayer of cells with EDTA-trypsin, stop the digestion with cell culture medium with a serum concentration of 0.5%, obtain the subcultured cell suspension according to the 1:1 passage ratio, and spread the cell suspension in 96-well cell culture plate, 50uL per well;

[0031] (2) Determination of the virus content of live vaccine for swine fever in passage cells

[0032] Inoculate ST cells with the attenuated strain of classical swine fever rabbitization, and store the harvested virus antigen solution at -80°C. Take the pre-cooled MEM medium at 4°C, and dilute the live swine fever vaccine by 10 times on the ice box. Dilute 10 -2 ~10 -6 Add the diluted virus suspension to the cell...

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Abstract

The invention belongs to the technical field of biological quarantine and particularly relates to a method for detecting the virus content of a swine fever live vaccine through indirect immunofluorescence. The method for detecting the virus content of the swine fever live vaccine through indirect immunofluorescence comprises the following steps: (1) preparing subculture cells; (2) measuring the virus content of the swine fever live vaccine in the subculture cells; (3) fluorescently dyeing virus-inoculated cells. According to the method, the viruses of the live vaccine in the subculture cells are detected through indirect immunofluorescence so as to achieve the purpose of rapidly, accurately, simply and effectively detecting the virus content of the swine fever live vaccine.

Description

technical field [0001] The invention belongs to the technical field of biological quarantine, and in particular relates to a method for detecting virus content of swine fever live vaccine by using indirect immunofluorescence. Background technique [0002] Classical swine fever (CSF or hog cholera), also known as classical swine fever or classical swine fever, is a highly contagious disease that infects pigs. Swine fever can cause fever, anorexia, diarrhea, death, etc. in sick pigs, and may have neurological symptoms. Sows may miscarry or give birth to dead piglets. Swine fever is one of the 16 legal infectious diseases of Class A listed by the World Organization for Animal Health, and it is designated as a severe infectious disease in China. Mandatory immunization with swine fever live vaccines is currently the best way to prevent swine fever in many countries and regions. [0003] At present, the detection methods for the virus content of live swine fever vaccines produc...

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Application Information

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IPC IPC(8): G01N33/569G01N21/64
CPCG01N33/56983
Inventor 杨灵芝郭显坡王朝伟张静程小囯
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH
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