The invention relates to a method for preparing
auricularia auricula
polysaccharide through
fermentation of eurotium cristatum. The black
fungus is crushed and sieved, and then mixed with
distilled water for autoclaved sterilization. After cooling, adding 5% (v / w) of eurotium cristatum, and fermenting at 28 DEG C; after
fermentation is finished,
distilled water is added, and ultrasonic treatment is performed after uniform stirring. Then, adjusting the pH value to 9-11, magnetically stirring for 0.5-2 hours, performing
centrifugal separation, collecting supernate, and concentrating to 1 / 4-1 / 8 of the original volume; adjusting the pH value to 2.0-5.0, carrying out cold
precipitation in a refrigerator at 4 DEG C for 12-36 hours, centrifuging again, collecting supernate, and adding absolute ethyl
alcohol to precipitate
polysaccharide. And finally, freeze-
drying the precipitate to obtain the
auricularia auricula
polysaccharide. The yield of the fermented polysaccharide is 8.175%, when the concentration of the fermented polysaccharide is 2.0 mg / mL, the
scavenging rates of the fermented polysaccharide on
DPPH and
superoxide anion free radicals are 63.74% and 57.11% respectively, and the inhibition rate of alpha-
amylase is 69.57%. The method is simple in process, the obtained
auricularia auricula polysaccharide is high in yield and
biological activity, and the method is suitable for large-scale production and is widely applied to the fields of food,
medicine and health care products.