A method for artificial breeding of scutellerus tenuipes

Abstract

This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

Description

Technical Field

[0001] This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to an artificial breeding method for the trapezoidal assassin bug. Background Technology

[0002] Cone-shielded rhomboid assassin bug ( Isyndus reticulatus Belonging to the order Hemiptera, family Reduviidae, and subfamily Harpactorinae, this is a relatively large, diurnal insect that preys on natural enemies. It has significant control potential against a variety of important agricultural and forestry pests, including the teak moth (…). Hyblaean girls ), pine caterpillars ( Dendrolimus punctatus ), Camellia geometrid moth ( Pine-horned cow ),cabbage caterpillar( Pieris turnips ) and pine aphid ( Cinara formosana Lepidoptera and Hemiptera pests, including the teak moth, have begun to cause significant damage to Avicennia marina (a type of mangrove) in coastal mangrove forests of my country due to recent global climate change and host introductions. Sea cucumber ) and precious tree species teak ( Large tectona Mangrove ecosystems are rich in biodiversity and are not suitable for pest control using chemical pesticides. Therefore, relying on the National Key Research and Development Program "Research on Key Technologies for Biosafety (2023YFC2604804)", the author conducted a comprehensive survey of natural enemies of the teak moth in mangrove areas in Yunnan, Guangxi Zhuang Autonomous Region, Guangdong, and Hainan provinces. Multiple observations of the trapezoidal assassin bug preying on the larvae of the teak moth were made, indicating its promising application prospects in green pest control and its potential to be developed into an effective natural enemy product against various important agricultural and forestry pests.

[0003] Hoffmann (1935) first reared this insect under laboratory conditions in Guangdong Province, recording its life history and describing and illustrating the morphological characteristics of adults and nymphs, but did not cover details such as the number of generations and ecological behavior. (See the literature "Bionomic notes of...") Isyndus reticulatusThe biological characteristics of the insect, described in the report "Stål (Hemiptera: Reduviidae)," include its bivoltine life cycle with five nymphal instars, as well as its predatory behavior, mating behavior, oviposition, hatching and molting processes, aggregation, and cannibalism. However, the artificial breeding of the *Hemiptera rubescens* still faces several technical challenges: First, high mortality rates among young nymphs: newly hatched nymphs have weak environmental adaptability and exhibit cannibalism, leading to low survival rates. Second, unstable mating success rates: adult mating behavior in artificial environments is influenced by various factors, and standardized mating induction methods are lacking. Third, low egg mass hatching rates: egg masses easily lose water and shrivel in dry environments, affecting hatching rates. Fourth, lack of a year-round breeding system: under natural conditions, the second-generation adults need to overwinter, making continuous year-round breeding difficult. Therefore, developing a simple, high-survival-rate, and scalable artificial breeding method for *Hemiptera rubescens* is of great significance for promoting its application in the biological control of agricultural and forestry pests. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides an artificial breeding method for the trapezoidal assassin bug, enabling large-scale, year-round production of this natural enemy insect and providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a method for the artificial breeding of the Triplophysa fasciata, comprising the following steps: 1) Adult assassin bugs of the cone-shaped shield were raised in a breathable insect rearing cage; 2) Nymphs bred from adult assassin bugs described in step 1) are raised to adulthood in a breeding environment; The conditions of the breeding environment include: temperature of 20~25℃, relative humidity of 55~65%, light cycle of 14 hours of light and 10 hours of darkness, light intensity of 2000~3000 Lux, and ventilation 2~3 times a day for 30 minutes each time. The feeding conditions for the nymphs include: feeding 1st and 2nd instar nymphs with mealworm larvae or aphids with a body length of 3-5 mm; feeding 3rd and 4th instar nymphs with mealworm larvae with a body length of 8-12 mm; feeding 5th instar nymphs with mealworm larvae or pupae with a body length of 15-20 mm; feeding once every 3 days, with each nymph producing 1-2 mealworm larvae; The adults are fed mealworm larvae or pupae with a body length of 15-20 mm; they are fed once every 3 days, and each adult produces 1-2 mealworm larvae. 3) Mating the adult insects obtained from step 2) at a female-to-male ratio of 1:1 to 2; 4) Place the mated female insect from step 3) into an oviposition box to lay eggs; The dimensions of the spawning box are 20cm×15cm×15cm, and bark strips or folded corrugated cardboard strips are placed inside the box as the spawning medium. 5) Place the eggs obtained in step 4) into an incubation box to hatch, and obtain nymphs; The incubation box has the following specifications: 10cm in diameter and 8cm in height. It is a transparent plastic box with a damp sponge or cotton lining at the bottom to maintain a relative humidity of 70-80%. 6) Separate the nymphs obtained in step 5) and raise them according to their age.

[0006] Preferably, the collection criteria for adult Triplophysa fasciata in step 1) are: body length 18-25mm, body color dark brown to black, forewings covering the abdomen, strong mobility, intact antennae and appendages, and no parasitic or disease symptoms. The dimensions of the breathable insect rearing cage are 30cm×30cm×40cm, with no more than 20 pairs per cage, and the insects are temporarily raised for 3-5 days.

[0007] Preferably, in step 2), the 1st and 2nd instar nymphs are raised in a transparent plastic insect rearing box with a diameter of 12cm and a height of 10cm. The 3rd and 5th instar nymphs are raised in a transparent plastic insect rearing box with a size of 45cm×35cm×35cm. The adult insects are raised in a transparent plastic insect rearing box with a size of 45cm×35cm×35cm. A 10cm×10cm mesh ventilation window is opened in the center of the lid of the transparent plastic insect rearing box, and water-absorbing cardboard or bark pieces are placed at the bottom of the box.

[0008] Preferably, the mating in step 3) is carried out in a mating box with dimensions of 20cm×15cm×15cm. Bark blocks or folded paper strips are placed inside the box as mating supports, and moist filter paper is laid at the bottom.

[0009] Preferably, in step 4), the number of female insects in each oviposition box is 1 to 2, and the length of the bark strip is 15 cm and the width is 2 to 3 cm.

[0010] Preferably, in step 5), the lid of the incubation box has ventilation holes with a diameter of 3cm made of mesh.

[0011] Preferably, the rearing density of the nymphs is no more than 15 per box.

[0012] Preferably, the rearing density of the adult insects is no more than 20 per box.

[0013] Preferably, when year-round breeding is required, the temperature is maintained at 25±2℃, with no low-temperature period, the photoperiod is maintained at 14L:10D, the light intensity is 2000~3000Lux, sufficient feed is continuously provided, and the humidity is maintained at 60±5%.

[0014] Preferably, the nymphs described in step 6) are separated within 24 hours after hatching.

[0015] The beneficial effects of this invention are: Age-separated rearing and cannibalism control techniques: Different rearing containers and density control standards are set for 1st to 5th instar nymphs. The nymphs are separated within 24 hours after hatching, which significantly improves the survival rate of young nymphs to over 80%.

[0016] Standardized mating induction method: By controlling the light and temperature cycle (25±2℃, 14L:10D), using a special mating box and support, and a suitable female-to-male ratio (1:1), mating behavior is promoted, and the mating success rate can reach over 85%.

[0017] Egg mass moisture-retaining incubation technology: Providing special egg-laying media such as bark strips and corrugated paper, combined with bottom moisture-retaining measures, increases the egg mass hatching rate from 60% under natural conditions to over 90%.

[0018] Year-round continuous breeding system: By controlling the temperature (25±2℃) and regulating the light (14L:10D), the natural overwintering dormancy is broken, and year-round breeding is achieved, increasing the number of breeding generations per year from 2 in nature to 4-5 generations. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0020] Figure 1 For egg mass incubation boxes; Figure 2 For rearing nymphs in their first and second instars; Figure 3 For rearing nymphs in their 3rd and 5th instars; Figure 4 This is a mating cage for adult insects. Detailed Implementation

[0021] This invention provides a method for the artificial breeding of the Triplophysa fasciata, comprising the following steps: 1) Adult assassin bugs of the cone-shaped shield were raised in a breathable insect rearing cage; 2) Nymphs bred from adult assassin bugs described in step 1) are raised to adulthood in a breeding environment; The conditions of the breeding environment include: temperature of 20~25℃, relative humidity of 55~65%, light cycle of 14 hours of light and 10 hours of darkness, light intensity of 2000~3000 Lux, and ventilation 2~3 times a day for 30 minutes each time. The feeding conditions for the nymphs include: feeding 1st and 2nd instar nymphs with mealworm larvae or aphids with a body length of 3-5 mm; feeding 3rd and 4th instar nymphs with mealworm larvae with a body length of 8-12 mm; feeding 5th instar nymphs with mealworm larvae or pupae with a body length of 15-20 mm; feeding once every 3 days, with each nymph producing 1-2 mealworm larvae; The adults are fed mealworm larvae or pupae with a body length of 15-20 mm; they are fed once every 3 days, and each adult produces 1-2 mealworm larvae. 3) Mating the adult insects obtained from step 2) at a female-to-male ratio of 1:1 to 2; 4) Place the mated female insect from step 3) into an oviposition box to lay eggs; The dimensions of the spawning box are 20cm×15cm×15cm, and bark strips or folded corrugated cardboard strips are placed inside the box as the spawning medium. 5) Place the eggs obtained in step 4) into an incubation box to hatch, and obtain nymphs; The incubation box has the following specifications: 10cm in diameter and 8cm in height. It is a transparent plastic box with a damp sponge or cotton lining at the bottom to maintain a relative humidity of 70-80%. 6) Separate the nymphs obtained in step 5) and raise them according to their age.

[0022] This invention involves rearing adult assassin bugs (such as *Triplophysa pulcherrima*) in ventilated rearing cages. The criteria for collecting adult *Triplophysa pulcherrima* are: body length 18-25 mm, dark brown to black body color, forewings covering the abdomen, strong mobility, intact antennae and appendages, and no parasitic or disease symptoms. After collection, the adults are placed in ventilated rearing cages (30cm×30cm×40cm), with no more than 20 pairs per cage, and temporarily reared for 3-5 days to acclimatize to the artificial environment.

[0023] This invention breeds nymphs of the adult Triplophysa fasciata into adults in a breeding environment. The breeding environment conditions include: a temperature of 20-25°C, a relative humidity of 55-65%, a light cycle of 14 hours of light and 10 hours of darkness, a light intensity of 2000-3000 Lux, and ventilation 2-3 times a day for 30 minutes each time. The feeding conditions for the nymphs include: feeding 1st and 2nd instar nymphs with mealworm larvae of 3-5 mm in length; feeding 3rd and 4th instar nymphs with mealworm larvae of 8-12 mm in length; feeding 5th instar nymphs with mealworm larvae or pupae of 15-20 mm in length; feeding once every 3 days, with each nymph producing 1-2 mealworm larvae; the adults are fed 15-20 mm in length mealworm larvae or pupae; feeding once every 3 days, with each nymph producing 1-2 mealworm larvae. In this invention, the 1st and 2nd instar nymphs are preferably raised in transparent plastic rearing boxes with a diameter of 12 cm and a height of 10 cm, and the 3rd to 5th instar nymphs are preferably raised in transparent plastic rearing boxes with a size of 45 cm × 35 cm × 35 cm. The adult insects are preferably raised in a transparent plastic insect rearing box with dimensions of 45cm×35cm×35cm. A 10cm×10cm mesh ventilation window is opened in the center of the lid of the transparent plastic insect rearing box, and water-absorbing cardboard or bark pieces are placed at the bottom of the box.

[0024] In this invention, the bred adult insects are mated at a female-to-male ratio of 1:1 to 2. Preferably, the mating takes place in a mating box measuring 20cm × 15cm × 15cm. Bark pieces or folded paper strips are placed inside the box as a mating support, and moist filter paper is laid at the bottom.

[0025] This invention involves placing mated female insects in an oviposition box to lay their eggs. The oviposition box has dimensions of 20cm × 15cm × 15cm, and bark strips or folded corrugated cardboard strips are placed inside as the oviposition medium. In this invention, the number of female insects in each oviposition box is preferably 1-2, and the length of the bark strip is preferably 15cm, and the width is preferably 2-3cm.

[0026] In this invention, the obtained eggs are placed in an incubation box for hatching to obtain nymphs. The incubation box has the following specifications: 10cm in diameter and 8cm in height. It is a transparent plastic box with a bottom lined with a damp sponge or cotton to maintain a relative humidity of 70-80%. In this invention, the lid of the incubation box preferably has a mesh ventilation hole with a diameter of 3cm.

[0027] In this invention, the nymphs are reared separately according to their age. Preferably, the nymphs are separated within 24 hours of hatching.

[0028] In this invention, the preferred rearing density of the nymphs is no more than 15 per box. In this invention, the preferred rearing density of the adults is no more than 20 per box.

[0029] In this invention, when year-round breeding is required, the temperature is maintained at 25±2℃, with no low-temperature period, the photoperiod is maintained at 14L:10D, the light intensity is 2000~3000Lux, sufficient feed is continuously provided, and the humidity is maintained at 60±5%. Overwintering dormancy is broken by adjusting environmental parameters.

[0030] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention. Example 1

[0031] The effects of different temperatures on growth and development This embodiment sets up three temperature gradients (20℃, 25℃, and 30℃), while keeping other conditions consistent (RH 60±5%, light intensity 14L:10D, feed consisting of mealworm larvae), to compare the developmental duration of each stage of the assassin bug *Triplophysa pulcherrima*. Experimental method: Ninety first-instar nymphs hatched on the same day were randomly divided into three groups of 30 each, and placed in constant-temperature incubators at 20℃, 25℃, and 30℃ respectively. Each nymph was individually reared in a transparent plastic cup with a diameter of 6cm and a height of 8cm. Moistened filter paper was placed at the bottom of the cup, and the cup lid had a mesh ventilation hole. Molting time and mortality were observed and recorded daily, and the developmental duration and survival rate of each instar were calculated. Adult lifespan was recorded after emergence. The results showed that the nymph survival rate was highest (86.7%) at 25℃, with a moderate developmental duration (36.1 days) and a relatively long adult lifespan (62.3 days), making it the most suitable temperature for artificial breeding. At 20℃, development is slow but adult lifespan is long, making it suitable for breeding; at 30℃, development is fast but survival rate and adult lifespan decrease.

[0032] Table 1. Effects of different temperatures on growth and development Example 2

[0033] Effects of different feeds on nymph survival rate This example compares the effects of mealworm larvae and mixed feed (mealworms + aphids) on the survival rate of *Trametes versicolor* assassin bug nymphs. Experimental method: Sixty first-instar nymphs hatched on the same day were randomly divided into two groups of 30 each. Group A: Fed with mealworm larvae throughout the entire instar period (selected according to appropriate size). Group B: Fed with the same number of peach aphids for first and second instar nymphs (… Myzus persica ) and bean aphid ( Aphis spicatus After the 3rd instar, mealworm larvae were fed. Rearing conditions: 25±2℃, RH 60±5%, light 14L:10D, each nymph was reared individually. Mortality was observed and recorded daily, and the survival rate of each instar and the overall nymphal survival rate were calculated. Results showed that feeding 1st and 2nd instar nymphs with aphids significantly improved the survival rate of younger nymphs, increasing the overall nymphal survival rate from 70.0% to 86.7%. This is because aphids are small and have soft body walls, making them more suitable prey for younger nymphs. However, aphids are relatively expensive to obtain; for large-scale breeding, it is advisable to use aphids for the 1st and 2nd instars and then switch to mealworms after the 3rd instar to balance cost and benefit.

[0034] Table 2 Effects of different feeds on nymph survival rate Example 3

[0035] Relationship between mating success rate and female-to-male ratio This embodiment sets different female-to-male ratios to compare the mating success rate of the assassin bug *Triplophysa pulcherrima*. Experimental method: Healthy adults 10-15 days after emergence were randomly divided into 3 groups, with 20 females in each group. Treatment 1: Female-to-male ratio 1:1 (20 females, 20 males); Treatment 2: Female-to-male ratio 2:1 (20 females, 10 males); Treatment 3: Female-to-male ratio 1:2 (20 females, 40 males). Each group was placed in a 45cm×35cm×35cm rearing cage, with bark pieces provided as supports, at 25±2℃, RH 60±5%, and a light intensity of 14L:10D, with ample mealworm larvae. Observations were conducted for 7 consecutive days, and the number of successfully mating females was recorded (confirmation was based on observed mating behavior or subsequent oviposition). Results showed that both female-to-male ratios of 1:1 and 1:2 achieved high mating success rates, but at a ratio of 1:2, competition among males was intense, with occasional fighting. Taking into account both resource utilization efficiency and mating success rate, a male-to-female ratio of 1:1 is the optimal choice.

[0036] Table 3 Relationship between mating success rate and female-to-male ratio Example 4

[0037] The effect of egg mass moisturizing methods on hatching rate This embodiment compares the effects of different moisturizing methods on the hatching rate of *Triplophysa pulcherrima* egg masses. Experimental method: Thirty egg masses (each containing 40-60 eggs) laid on the same day were collected and randomly divided into three groups of 10 masses each. Treatment 1 (sponge method): A sponge with 70-80% humidity was placed at the bottom of the hatching box, and the egg masses were suspended on a mesh screen, not in direct contact with water. Treatment 2 (spray method): Water was sprayed twice daily, morning and evening, to keep the surface of the egg masses moist. Treatment 3 (dry control): No moisturizing treatment was applied; the indoor humidity was at approximately 40-50%. Hatching conditions: 25±2℃, light intensity 12L:12D. Hatching was observed daily, and the number of hatched nymphs was recorded. Results showed that the sponge method achieved the highest hatching rate (92.0%), and the egg masses showed no mold growth. Although the spray method provided higher humidity, it easily led to mold growth on the surface of the egg masses, reducing the hatching rate. Under the dry control conditions, the egg masses lost a lot of water, and the hatching rate was only 61.8%. Therefore, the wet cotton ball method is preferred for moisturizing and hatching egg masses.

[0038] Table 4. Effects of egg mass moisture retention methods on hatching rate Example 5

[0039] The effect of age-separated rearing on nymph survival rate This embodiment compares the effects of mixed rearing and age-separated rearing on the survival rate of *Trametes versicolor* assassin bug nymphs. Experimental methods: Nymphs of different ages (30 each of 1st, 3rd, and 5th instars) were selected and set up in three groups as follows: Treatment 1 (mixed rearing): 10 nymphs each of 1st, 3rd, and 5th instars were mixed and placed in a large box (45cm×35cm×35cm). Treatment 2 (age-separated rearing): 30 nymphs each of 1st, 3rd, and 5th instars were placed in small boxes (12cm in diameter, 10cm in height), with uniform density in each box. Treatment 3 (individual rearing): 30 1st instar nymphs were placed individually in small cups (6cm in diameter, 8cm in height). Rearing conditions: 25±2℃, RH 60±5%, light 14L:10D, with sufficient mealworm larvae. Observations were conducted for 15 consecutive days, mortality was recorded, and survival rate was calculated. The results showed that when reared in a mixed manner, the survival rate of first-instar nymphs was only 30.0%, and it was clearly observed that third- and fifth-instar nymphs preyed on first-instar nymphs. Separate rearing by age significantly improved the survival rate of each instar, with an overall survival rate of 90.0%. Individual rearing achieved the highest survival rate (96.7%), but required more space and incurred higher management costs. Therefore, separate rearing by age should be adopted for large-scale breeding to avoid mixing individuals of different ages.

[0040] Table 5. Effects of age-specific rearing on nymph survival rate

[0041] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for artificial breeding of Panstrongylus megistus, characterized by, Includes the following steps: 1) The adult assassin bugs of the cone-shaped water chestnut were raised in a breathable insect rearing cage; 2) Nymphs bred from adult assassin bugs described in step 1) are raised to adulthood in a breeding environment; The conditions of the breeding environment include: temperature of 20~25℃, relative humidity of 55~65%, light cycle of 14 hours of light and 10 hours of darkness, light intensity of 2000~3000 Lux, and ventilation 2~3 times a day for 30 minutes each time. The feeding conditions for the nymphs include: feeding 1st and 2nd instar nymphs with mealworm larvae or aphids with a body length of 3-5 mm; feeding 3rd and 4th instar nymphs with mealworm larvae with a body length of 8-12 mm; feeding 5th instar nymphs with mealworm larvae or pupae with a body length of 15-20 mm; feeding once every 3 days, with each nymph producing 1-2 mealworm larvae; The adults are fed mealworm larvae or pupae with a body length of 15-20 mm; they are fed once every 3 days, and each adult produces 1-2 mealworm larvae. 3) Mating the adult insects obtained from step 2) at a female-to-male ratio of 1:1 to 2; 4) Place the mated female insect from step 3) into an oviposition box to lay eggs; The egg-laying box has the following dimensions: 20cm×15cm×15cm. Bark strips or folded corrugated cardboard strips are placed inside the box as the egg-laying medium. 5) Place the eggs obtained in step 4) in an incubation box to hatch, and obtain nymphs; The incubation box has the following specifications: 10cm in diameter and 8cm in height. It is a transparent plastic box with a damp sponge or cotton lining at the bottom to maintain a relative humidity of 70-80%. 6) Separate the nymphs obtained in step 5) and raise them according to their age.

2. The artificial breeding method according to claim 1, characterized by, Step 1) The collection criteria for adult Triplophysa fasciata are: body length 18-25mm, body color dark brown to black, forewings covering the abdomen, strong mobility, intact antennae and appendages, and no parasitic or disease symptoms. The dimensions of the breathable insect rearing cage are 30cm×30cm×40cm, with no more than 20 pairs per cage, and the insects are temporarily raised for 3-5 days.

3. The artificial breeding method according to claim 1, characterized by, Step 2) The 1st and 2nd instar nymphs are raised in transparent plastic insect rearing boxes with a diameter of 12cm and a height of 10cm. The 3rd and 5th instar nymphs are raised in transparent plastic insect rearing boxes with a size of 45cm×35cm×35cm. The adult insects are raised in transparent plastic insect rearing boxes with a size of 45cm×35cm×35cm. A 10cm×10cm mesh ventilation window is opened in the center of the lid of the transparent plastic insect rearing box, and water-absorbing cardboard or bark pieces are placed at the bottom of the box.

4. The artificial breeding method according to claim 1, characterized by, Step 3) The mating takes place in a mating box with dimensions of 20cm×15cm×15cm. Bark pieces or folded paper strips are placed inside the box as mating supports, and moist filter paper is laid at the bottom.

5. The artificial breeding method according to claim 1, characterized by, Step 4) The number of female insects in each oviposition box is 1 to 2, and the length of the bark strip is 15cm and the width is 2 to 3cm.

6. The artificial breeding method according to claim 1, characterized by, Step 5) The lid of the incubation box has a mesh ventilation hole with a diameter of 3cm.

7. The artificial breeding method according to claim 1, characterized by, The rearing density of the nymphs is no more than 15 per box.

8. The artificial breeding method according to claim 1, characterized by, The rearing density of the adult insects is no more than 20 per box.

9. The artificial breeding method according to claim 1, characterized by, When year-round breeding is required, the temperature should be maintained at 25±2℃, with no low-temperature period, a photoperiod of 14L:10D, a light intensity of 2000~3000Lux, a continuous supply of sufficient feed, and a humidity of 60±5%.

10. The artificial breeding method according to claim 1, characterized by, Step 6) The nymphs are separated within 24 hours after hatching.

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