Application of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene or myristyl acetate in attracting green mirid bugs

Abstract

This invention relates to the application of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene or myristyl acetate in attracting green mirid bugs. Specifically, (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene has an attractive effect on nymphs, female adults, and male adults of the green mirid bug; furthermore, myristyl acetate can enhance the attraction of TMTT to nymphs, female adults, and male adults of the green mirid bug.

Description

Technical Field

[0001] This invention relates to the field of biological control, and particularly to the field of attractants. Background Technology

[0002] The green mirid bug (Apolygus lucorum), belonging to the family Miridae in the order Hemiptera and the genus Apolygus, is a significant agricultural pest widely distributed across many regions of my country, with the most severe damage occurring in the Yangtze and Yellow River basins. It is an omnivorous pest, hosting over 200 species of plants from 50 families, including cotton, jujube, tea, various fruit trees, and vegetables, and its damage is on the rise. The green mirid bug is a piercing-sucking insect; both adults and nymphs can pierce and suck the tender leaves, buds, and fruits of plants. Since the widespread use of Bt genetically modified insect-resistant cotton, the reduced use of broad-spectrum insecticides has led to a lack of timely control of the green mirid bug. Furthermore, the green mirid bug's strong environmental adaptability, high population growth rate, and strong dispersal ability make it prone to reaching outbreak densities, thus gradually elevating it from a secondary pest to a major one, posing a serious threat to cotton and various crops in my country. Furthermore, the green mirid bug is difficult to control in the field due to its high activity level, nocturnal activity, and attraction to flowers and tender shoots. Moreover, long-term use of chemical control methods has led to the rapid development of pesticide resistance. Therefore, there is an urgent need to develop new pest control methods to effectively combat the damage caused by mirid bugs. Summary of the Invention

[0003] One aspect of the present invention provides the use of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene and / or myristyl acetate in the attraction of green mirid bugs.

[0004] In one specific embodiment, the green mirid bug is at least one of a nymph, a male adult, and a female adult.

[0005] The second invention provides a composition comprising (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene and myristyl acetate.

[0006] In one specific embodiment, the mass ratio of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene to myristyl acetate is 1:(0.3 to 1.5).

[0007] In one specific embodiment, the mass ratio of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene to myristyl acetate is 1:1.

[0008] In one embodiment, the composition further includes a pesticide-acceptable carrier.

[0009] In one specific embodiment, the pesticide-acceptable carrier includes a solvent.

[0010] The third invention provides a decoy comprising the composition and sustained-release carrier as described in any one of the second inventions.

[0011] In one specific embodiment, the sustained-release carrier is made of rubber.

[0012] In one specific embodiment, the sustained-release carrier is at least one of a rubber-tip carrier, a cotton-core carrier, and a microtubule carrier.

[0013] The fourth invention provides the application of the composition described in any one of the second inventions or the lure described in the third invention in attracting green mirid bugs.

[0014] In one specific embodiment, the green mirid bug is at least one of a nymph, a male adult, and a female adult.

[0015] Beneficial effects of the present invention: The present invention discovers that (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) has an attractive effect on nymphs, female adults, and male adults of the green mirid bug. Furthermore, the present invention also discovers that myristyl acetate (MA) can enhance the attractiveness of TMTT to nymphs, female adults, and male adults of the green mirid bug. Attached Figure Description

[0016] Figure 1 A schematic diagram of a Y-shaped olfactory sensor test is shown.

[0017] Figure 2 The behavioral response of the green mirid bug was shown when both arms of the Y-shaped olfactory apparatus contained n-hexane. NS indicates no significant difference at the P > 0.05 level.

[0018] Figure 3 The results of the Y-type olfactory sensor tests in Examples 2 to 4 are shown. Here, NS indicates no significant difference at the P > 0.05 level; * indicates a significant difference at the P < 0.05 level; *** indicates a significant difference at the P < 0.001 level.

[0019] Figure 4 The results of Y-type olfactometer tests in Examples 5 to 7 are shown. * indicates a significant difference at the P < 0.05 level; ** indicates a significant difference at the P < 0.01 level. Detailed Implementation

[0020] The present invention will be further described in detail below through preferred embodiments, but these embodiments do not constitute a limitation thereof.

[0021] Unless otherwise specified, all reagents used in the embodiments of this invention are commercially available.

[0022] (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT, density 0.814 g / mL) was purchased from Beijing Bailingwei Technology Co., Ltd. (J&KScientific).

[0023] Myristyl acetate (MA, density 0.746 g / mL) was purchased from J&K Scientific, Beijing.

[0024] The nymphs and adults of the green mirid bug were fed fresh corn kernels (Zea mays) or a mixture of corn kernels and green beans (Phaseolus vulgaris L.) at a temperature of 27±1℃, a relative humidity of 65±5%, and a light ratio of 16:8 (L:D).

[0025] The behavioral responses of 5th instar nymphs and male and female adults on the 6th day after emergence of the green mirid bug were tested using a Y-type olfactory instrument to odor compounds.

[0026] Example 1

[0027] like Figure 1 As shown, the Y-type olfactory device is a colorless, transparent glass tube with a diameter of 1 cm. The main arm and both side arms are 10 cm long, and the angle between the two side arms is 90°. During the experiment, a QC-1B air sampler (Beijing Institute of Labor Protection) was used to provide the airflow. The gas supplied by the air sampler first passed through activated carbon filtration, then was humidified by a humidification tower, and finally flowed through a flow meter to the two side arms of the Y-type olfactory device. The airflow velocity through the two side arms was 400 mL / min, the indoor temperature was controlled at 26±1℃, and the experiment lasted from 8:00 to 19:00.

[0028] First, a hexane negative control experiment was conducted to ensure that there was no difference in the selection of the left and right arms of the Y-shaped olfactory instrument between green mirid bug nymphs and adults, thus ruling out the device's stable use in behavioral selection experiments involving green mirid bugs. The specific operation is as follows:

[0029] 1) The green mirid bugs were starved for 6 hours before the experiment.

[0030] 2) Add 20 μL of n-hexane to a 1 cm × 1 cm filter paper and place it at position A on the left arm of the Y-shaped olfactory instrument. Add 20 μL of n-hexane to another filter paper of the same size and place it at position B on the right arm.

[0031] 3) Turn on the atmospheric sampler and adjust the flow meter to ensure the airflow velocity through both arms is 400 mL / min, maintaining a consistent flow velocity across both arms. Place the green mirid bug at the beginning of the main arm and start timing for 7 minutes. If a green mirid bug enters the left arm and extends more than 4 cm beyond it, remaining there for more than 1 minute, it is recorded as having selected the left arm. Similarly, if it enters the right arm and extends more than 4 cm beyond it, remaining there for more than 1 minute, it is recorded as having selected the right arm. If it remains within the main arm, it is recorded as not selected. After testing 5 green mirid bugs, switch the Y-type olfactometer to reverse the positions of the left and right arms to eliminate the positional effect on the selection of green mirid bugs. Replace the Y-type olfactometer after testing 10 green mirid bugs. At least 45 bugs should be tested for each developmental stage.

[0032] After the experiment, the Y-type olfactory instrument was cleaned with acetone, treated with 75% ethanol, and then dried in a constant temperature drying oven at 120℃ for 2 hours.

[0033] The number of left and right arms selected by nymphs and adult males and females of the green mirid bug was counted. A chi-square test was used to analyze the significance of the data. The results are shown below. Figure 2 .

[0034] Figure 2 The results showed that there was no significant difference in the selection of the left and right arms of the Y-shaped olfactory sensor.

[0035] Example 2

[0036] A TMTT mother liquor with a concentration of 100 μg / μL was prepared using n-hexane as a solvent.

[0037] The TMTT stock solution was sequentially diluted with n-hexane to 10 μg / μL, 1 μg / μL, and 1×10 μg / μL, according to a concentration gradient. -1 μg / μL and 1×10 -2 μg / μL TMTT solution.

[0038] The differences from Example 1 when performing Y-tube testing are as follows:

[0039] Add 20 μL of n-hexane to a 1 cm × 1 cm filter paper and place it at position A on the left arm of a Y-shaped olfactory instrument. Add 20 μL of 1 × 10⁻⁶ hexane to another filter paper of the same size. -2 A TMTT solution of μg / μL was placed at position B on the right arm.

[0040] Finally, the numbers of green mirid bug nymphs and adult females selected for hexane and TMTT were counted, and the chi-square test was used to analyze the significance of the data. The results are shown in [Figure number missing]. Figure 3 .

[0041] Example 3

[0042] A mother liquor of MA with a concentration of 100 μg / μL was prepared using n-hexane as a solvent.

[0043] The MA stock solution was successively diluted with n-hexane to 10 μg / μL, 1 μg / μL, and 1×10 μg / μL according to the concentration gradient. -1 μg / μL and 1×10 -2 A μg / μL MA solution.

[0044] The differences from Example 1 when performing Y-tube testing are as follows:

[0045] Add 20 μL of n-hexane to a 1 cm × 1 cm filter paper and place it at position A on the left arm of a Y-shaped olfactory instrument. Add 20 μL of 1 × 10⁻⁶ hexane to another filter paper of the same size. -2 A solution of 1 μg / μL MA was placed at position B on the right arm.

[0046] Finally, the numbers of green mirid bug nymphs and adult females selected for hexane and MA were counted. The chi-square test was used to analyze the significance of the data. The selection results are shown below. Figure 3 .

[0047] Example 4

[0048] Take 100 μL 1×10 -2 μg / μL TMTT solution and 100μL 1×10 -2 The MA solution of μg / μL was thoroughly mixed in a 1:1 ratio to obtain mixed solution 1#.

[0049] The differences from Example 1 when performing Y-tube testing are as follows:

[0050] Add 20 μL of n-hexane to a 1 cm × 1 cm filter paper and place it at position A on the left arm of the Y-shaped olfactory instrument. Add 20 μL of mixed solution #1 to another filter paper of the same size and place it at position B on the right arm.

[0051] Finally, the number of green mirid bug nymphs and adult females in hexane and mixture #1 were counted. A chi-square test was used to analyze the significance of the data. The results are shown below. Figure 3 .

[0052] Example 5

[0053] The difference from Example 3 is that:

[0054] Add 20 μL of mixed solution #1 to a 1 cm × 1 cm filter paper and place it at position A on the left arm of the Y-shaped olfactory instrument. Add 20 μL of 1 × 10⁻⁶ solution to another filter paper of the same size. -2 A TMTT solution of μg / μL was placed at position B on the right arm.

[0055] Finally, the number of green mirid bug nymphs and adult females in mixed solutions #1 and TMTT were counted. The chi-square test was used to analyze the significance of the data. The results are shown below. Figure 4 .

[0056] Example 6

[0057] Take 100 μL 1×10 -2 μg / μL TMTT solution and 30μL 1×10 -2 The MA solution of μg / μL was thoroughly mixed at a ratio of 1:0.3 to obtain mixed solution 2#.

[0058] The differences from Example 1 when performing Y-tube testing are as follows:

[0059] Add 20 μL of mixed solution #2 to a 1 cm × 1 cm filter paper and place it at position A on the left arm of the Y-shaped olfactory instrument. Add 20 μL of 1 × 10⁻⁶ solution to another filter paper of the same size. -2 A TMTT solution of μg / μL was placed at position B on the right arm.

[0060] Finally, the number of green mirid bug nymphs and adult females in mixed solutions #1 and TMTT were counted. The chi-square test was used to analyze the significance of the data. The results are shown below. Figure 4 .

[0061] Example 7

[0062] Take 50 μL 1×10 -2 μg / μL TMTT solution and 75μL 1×10 -2 The MA solution of μg / μL was thoroughly mixed in a ratio of 1:1.5 to obtain mixed solution 3#.

[0063] The differences from Example 1 when performing Y-tube testing are as follows:

[0064] Add 20 μL of mixed solution #3 to a 1 cm × 1 cm filter paper and place it at position A on the left arm of a Y-shaped olfactory instrument. Add 20 μL of solution #10 to another filter paper of the same size. -2 A TMTT solution of μg / μL was placed at position B on the right arm.

[0065] Finally, the number of green mirid bug nymphs and adult females in mixed solutions #1 and TMTT were counted. The chi-square test was used to analyze the significance of the data. The results are shown below. Figure 4 .

[0066] Figure 3 The results showed that TMTT could attract the nymphs, female adults, and male adults of the green mirid bug; MA could not attract the nymphs, female adults, and male adults of the green mirid bug; Mixture 1# could attract the nymphs, female adults, and male adults of the green mirid bug, and was superior to TMTT in attracting the green mirid bug, indicating that MA could enhance the attraction of green mirid bugs by TMTT.

[0067] Figure 4 The results showed that the attraction of mixtures 1# to 3# to nymphs, female adults and male adults of green mirid bugs was significantly stronger than that of TMTT; among them, the attraction of mixture 1# to nymphs, female adults and male adults of green mirid bugs was significantly stronger than that of mixtures 2# and 3#.

Claims

1. A composition comprising (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene and myristyl acetate, wherein the mass ratio of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene to myristyl acetate is 1:(0.3 to 1.5).

2. The composition according to claim 1, characterized in that, The mass ratio of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracetetraene to myristyl acetate is 1:

1.

3. The composition according to claim 1, characterized in that, The composition also includes a pesticide-acceptable carrier.

4. The composition according to claim 3, characterized in that, The pesticide-acceptable carriers include solvents.

5. A decoy comprising the composition as described in any one of claims 1 to 4 and a sustained-release carrier.

6. The lure core according to claim 5, characterized in that, The sustained-release carrier is made of rubber.

7. The lure core according to claim 5, characterized in that, The sustained-release carrier is at least one of rubber-tip carrier, cotton-core carrier, and microtubule carrier.

8. The use of the composition according to any one of claims 1 to 4 or the lure according to any one of claims 5 to 7 in attracting green mirid bugs.

9. The application according to claim 8, characterized in that, The green mirid bug is at least one of nymphs, male adults, and female adults.

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