A method for simultaneously preparing nutritious fruit powder and white wine or alcohol by fermenting fruits and vegetables
A fruit and vegetable fermentation and nutritional technology, applied in biochemical equipment and methods, microbiological-based methods, food preparation, etc., can solve the problems of not using large-scale production, distilling waste water to pollute the environment, and not forming a market, etc., to achieve product additional Value improvement, environmental pollution prevention, and utilization rate improvement effects
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Embodiment 1
[0030] 1. Preparation of raw materials: the ponkans harvested in Quzhou area, after selection and classification, weighed 150kg of raw materials, peeled and beaten the whole pulp directly to obtain 118.5kg of crude citrus pulp, and take out 50kg for use.
[0031] Fermentation of debittering enzyme:
[0032] After 5 days of Aspergillus niger WZ001 (CCTCC NO: M 206047) strain was activated by Chase medium, inoculate one loop to liquid seed medium (4g bran, 3g soybean meal, 8g soybean dregs, water 100mL) and cultivate for 36h to obtain Seed liquid.
[0033] The solid fermentation medium is 100g bran, 10g soybean dregs, and 20g soybean cake powder. Add 100ml of water and divide them into 500ml Erlenmeyer flasks. The filling thickness is 2.5~3.0cm. Steam pressure sterilization is 0.1Mpa for 30min. The bottle is connected with Aspergillus niger seed liquid at a volume ratio of 2%, and cultured at 32°C for 5-7 days. After the culture is dried, it is extracted and filtered with 1:10 water t...
Embodiment 2
[0044] 1. Preparation of raw materials: The ponkans harvested in Quzhou area are squeezed and filtered to obtain 6t of orange juice. Add liquid sulfur dioxide at 50g / T and save the orange juice for later use.
[0045] 2. Add 6kg of compound enzyme preparation to the crude citrus, and the enzyme treatment time is 90min. Acid pectinase: enzyme activity 30000u / g, purchased from Wuxi Xuemei Preparation Technology Co., Ltd.; cellulase: enzyme activity 40000u / g, purchased from Shandong Longda Bioengineering Co., Ltd.
[0046] 3. 5kg of super brewing high-activity dry yeast (Angel Yeast Co., Ltd.) was added to 300kg of orange juice, aeration was maintained at 30°C in a 1t fermenter and stirred and activated for 2h, as seed liquid.
[0047] 4. Preparation of citrus juice: add about 300kg rice saccharification solution to 6t orange juice (preparation method of saccharification solution: 350kg rice crushed, add water to 1000kg, add heat-resistant amylase, heat to 100℃, keep for 30min, cool to ...
Embodiment 3
[0054] 1. Preparation of raw materials: commercial oranges are peeled and then squeezed to obtain orange juice and orange residue.
[0055] Preservation and expansion of yeast strains:
[0056] Incline preservation medium: peptone 1g, yeast extract 1g, NaCl 0.4g, glucose 1g, agar 2g, water 100mL, pH 6.5, high temperature sterilization, cooling to make an inclined plane. Access to Huniang 2.089 fruit wine yeast (Shanghai Difa Brewing Biological Products Co., Ltd.) strains, cultivate at a constant temperature of 30°C for 2 to 3 days, and store at 4°C.
[0057] Primary seed culture medium: glucose 10g, peptone 1g, yeast extract 1g, MgSO 4 ·7H 2 O0.02g, KH 2 PO 4 0.02g, 100mL water, pH5.0. Inoculate a ring of slant strains at a culture temperature of 30°C, a shaker speed of 200r / min, and a culture time of 18h to obtain a first-level seed solution.
[0058] Secondary seed tank culture medium: glucose 10g, peptone 1g, yeast extract 1g, MgSO 4 ·H 2 O 0.02g, KH 2 PO 4 0.02g, 100mL water, p...
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