Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide

A technology for separation and purification of green tea, applied in applications, food preparation, blood diseases, etc., can solve problems affecting the biological activity of polysaccharides, human injury, environmental pollution, etc., and achieve simple and feasible operation, low energy consumption, and avoid environmental and human harm damage effect

Inactive Publication Date: 2013-01-23
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

The currently published patents mainly use the Sevag method and the trichloroacetic acid method for the removal of proteins in polysaccharide separation and purification. Human injury ...

Method used

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  • Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide
  • Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide
  • Method for preparing green tea polysaccharide through separation and purification and application of polysaccharide

Examples

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Embodiment 1

[0040] The tea leaves are pulverized by a pulverizer and passed through a 60-mesh sieve. The pulverized tea powder and distilled water are extracted at 30°C for 30 minutes at a weight ratio of 1:10. The extract is filtered to remove impurities and concentrated to 1% of the volume of the extract after removal of impurities. / 5, then slowly add concentrated and extracted

[0041] 3 times the volume of absolute ethanol, ethanol precipitation for 12 hours, centrifuged to remove the supernatant, and freeze-dried at -40°C to obtain crude polysaccharides.

[0042]Dissolve the prepared crude polysaccharide in distilled water, centrifuge for 10min at a speed of 3600r / min and put it on a polyamide column, use distilled water as the mobile phase at a flow rate of 1.0ml / min, collect the polysaccharide solution, and concentrate the collected polysaccharide solution to 1 / 5 of the original volume, then slowly add anhydrous ethanol with a volume 3 times the volume of the concentrated polysacc...

Embodiment 2

[0050] The tea leaves are pulverized by a pulverizer and passed through a 100-mesh sieve. The pulverized tea powder and distilled water are extracted at 70°C for 150 minutes at a weight ratio of 1:40. The extract is filtered to remove impurities and concentrated to 1% of the volume of the extract after removal of impurities. / 4, and then slowly add 4 times the volume of absolute ethanol of the concentrated extract, ethanol precipitation for 24 hours, centrifuge to remove the supernatant, and freeze-dry at -80°C to obtain crude polysaccharides.

[0051] Dissolve the prepared crude polysaccharide in distilled water, centrifuge for 20min at a speed of 4000r / min and put it on a polyamide column, use distilled water as the mobile phase at a flow rate of 2.0ml / min, collect the polysaccharide solution, and concentrate the collected polysaccharide solution to 1 / 4 of the original volume, then slowly add absolute ethanol with a volume 4 times the volume of the concentrated polysaccharide...

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Abstract

The invention relates to a method for preparing a green tea polysaccharide through separation and purification and application of the polysaccharide in the preparation of anticoagulant health products and medicaments. The preparation method comprises the following steps: crushing green tea, leaching, performing alcohol precipitation, and performing vacuum freeze-drying to obtain a crude polysaccharide; performing dynamic decolorization and deproteinization through a polyamide column; and further purifying through a DEAE (diethylaminoethyl) sepharose CL-6B ion exchange column to obtain the green tea polysaccharide. The invention has the following technical effects: pigments and proteins in the green tea crude polysaccharide are effectively removed; and the components obtained through separation and purification and other raw materials and auxiliary materials which are acceptable in food and medicament processes can be developed into appropriate health food preparations and medicament products having an anticoagulant function, such as capsules, tablets, oral liquids and the like. Experiment proves that the polysaccharide is safe and non-toxic, has a favorable anticoagulant function in the preparation of anticoagulant health products and medicaments and has favorable development and application prospects.

Description

technical field [0001] The invention relates to a method for separating and purifying green tea polysaccharides and its application, in particular to a method for separating and purifying green tea polysaccharides from crude old green tea and its application. Background technique [0002] With the improvement of people's living standards and the change of diet structure, hyperlipidemia and the thrombotic diseases caused by it have increasingly endangered people's health. Heparin polysaccharide has played a very important role in the treatment of thrombotic diseases, but it is easy to induce side effects such as thrombocytopenia. Find anticoagulant active ingredients from natural products, or use its main structure as a lead compound to develop new drugs, or develop functional Food has the advantages of low toxicity, low research and development costs, short cycle and universal health care. In our country and Japan, people have the experience of often drinking thick old tea ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L1/29A61K31/715A61P7/02A23L33/00
Inventor 蔡为荣谢亮亮
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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