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Primers and method for identifying greenhouse trialeurodes vaporariorum and bemisia tabaci by utilizing mitochondria SCAR (sequence characterized amplified regions) marker

A whitefly and Bemisia tabaci technology, which is applied in the field of agricultural biology and achieves the effect of simple and stable molecular markers

Inactive Publication Date: 2013-08-21
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of using the mitochondrial gene mt COI to identify whitefly and whitefly in greenhouses has not been reported yet.

Method used

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  • Primers and method for identifying greenhouse trialeurodes vaporariorum and bemisia tabaci by utilizing mitochondria SCAR (sequence characterized amplified regions) marker
  • Primers and method for identifying greenhouse trialeurodes vaporariorum and bemisia tabaci by utilizing mitochondria SCAR (sequence characterized amplified regions) marker
  • Primers and method for identifying greenhouse trialeurodes vaporariorum and bemisia tabaci by utilizing mitochondria SCAR (sequence characterized amplified regions) marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The whitefly described in Example 1 was collected in Shandong and Ningxia respectively in 2012; : 154-155.) to detect the above-mentioned whiteflies, and the whiteflies collected in Shandong and Ningxia were greenhouse whitefly and whitefly respectively.

Embodiment 2

[0035] The whiteflies described in Example 2 were collected in Gansu and Liaoning respectively in 2012; : 154-155.) to detect the above-mentioned whiteflies, and the whiteflies collected in Gansu and Liaoning were greenhouse whitefly and whitefly respectively.

Embodiment 3

[0036] The whitefly described in Example 3 was collected in Yunnan and Jilin respectively in 2012; : 154-155.) to detect the above-mentioned whitefly, and the whitefly collected in Yunnan and Jilin were greenhouse whitefly and whitefly respectively.

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Abstract

The invention relates to primers and method for identifying greenhouse trialeurodes vaporariorum and bemisia tabaci by utilizing mitochondria SCAR (sequence characterized amplified regions) marker. The primers are nucleotide sequences shown as SEQ ID NO.1 and SEQ ID NO.2 respectively. The method comprises the following steps: (1) extracting aleyrodid genome DNA (deoxyribonucleic acid); (2) performing polymerase chain reaction (PCR) amplification by taking the aleyrodid genome DNA as a template; and (3) performing sepharose gel electrophoretic analysis on the PCR amplification prepared in the step (2). According to the invention, a simple and stable molecular marker for identifying the greenhouse trialeurodes vaporariorum and bemisia tabaci is provided and a foundation is laid for research of population dynamic identification, biology and invasion mechanisms of aleyrodid in the future.

Description

technical field [0001] The invention relates to a primer and a method for using mitochondrial SCAR markers to identify whitefly and whitefly in greenhouses, in particular to a primer and a method for identifying whitefly and whitefly in greenhouses based on mitochondrial gene mt COI technology, and belongs to the field of agricultural biotechnology . Background technique [0002] Greenhouse whitefly Trialeurodes vaporariorum (Westwood) is an important worldwide pest in agriculture, native to North America, and now distributed all over the world. Greenhouse whitefly has strong fecundity, serious overlapping of generations, and a large population can be formed in a short time. Overwinter in the greenhouses of northern my country in various worm states and continue to harm, and more than 10 generations can occur in a year. More than 900 species of host plants have been reported, including tomato, tobacco, cucumber, eggplant, etc. The main ways of harm are: 1) Adults and nymp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 褚栋陶云荔国栋姜德锋
Owner QINGDAO AGRI UNIV
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