A kind of primer and method for distinguishing larvae of A. lepidus and A. sylvaticus using est microsatellite markers

A technology of microsatellite markers and primer pairs, which is applied in the field of agricultural biology and can solve problems such as the inability to construct EST microsatellite markers

Inactive Publication Date: 2016-06-29
QINGDAO AGRI UNIV
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  • Description
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  • Application Information

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Problems solved by technology

The main technical obstacle is that there is currently no transcriptome library of T. japonica larvae and Heterophthalmia larvae, so it is impossible to construct EST microsatellite markers, and the construction can distinguish T. The EST microsatellite markers of the larvae of Oculomyces elegans have a certain chance

Method used

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  • A kind of primer and method for distinguishing larvae of A. lepidus and A. sylvaticus using est microsatellite markers
  • A kind of primer and method for distinguishing larvae of A. lepidus and A. sylvaticus using est microsatellite markers
  • A kind of primer and method for distinguishing larvae of A. lepidus and A. sylvaticus using est microsatellite markers

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Chive tardigrades described in Example 1 were collected in 11 districts in Shandong Province in 2012;

Embodiment 2

[0027] In Example 2, A and B described in A and B were collected in 2012 respectively in Yiyuan District, Zibo City, Shandong Province and Changle District, Weifang City, Shandong Province;

Embodiment 3

[0028] A, B described in the embodiment 3 Chive tardigrade and Heterophthalmia were collected respectively in 2012 in Jining City, Shandong Province and Fei County, Linyi City, Shandong Province;

[0029] The above samples are all according to (Shi Baocai, Lu Hong, Gong Yajun, etc., 2010. Identification and control of Chive tardigrades. Chinese Vegetables, 11:21-22.; Zhang Hongrui, Zhang Xiaoyun, Shen Dengrong, etc., 2008. Edible fungi The biological characteristics of the fungus Bradysiadifformis. Chinese Edible Fungi, 27 (6): 54-56.) The above-mentioned Bradysiadifformis and different Bradysiadifformis were morphologically identified.

[0030] The instrument described in the embodiment is a Nikon SMZ745T stereo microscope, which is a common commercially available product.

[0031] Example 1

[0032] Constructed and sequenced the transcriptome library of T.

[0033] Firstly, the total RNA was extracted from the larvae of T. chileensis, and the extracted RNA was subjected to...

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Abstract

The invention relates to a method for identifying bradysia odoriphaga Yang et Zhang and bradysia difformis by EST (expressed sequence tag) microsatellite markers. The method comprises steps as follows: 1), bradysia odoriphaga Yang et Zhang gene DNA is extracted; 2), the bradysia odoriphaga Yang et Zhang gene DNA is taken as a template for PCR (polymerase chain reaction) amplification; and 3), a PCR product prepared from the step 2) is subjected to agarose gel electrophoresis analysis. According to the identification method, simple and stable molecular markers are provided for screening bradysia odoriphaga Yang et Zhang and bradysia difformis, and a foundation is established for future population dynamic identification, biology and control research of bradysia odoriphaga Yang et Zhang.

Description

technical field [0001] The invention relates to a primer and a method for using EST microsatellite markers to identify the larvae of the larvae of the larvae and the larvae of the leek, and belongs to the field of agricultural biotechnology. Background technique [0002] Bradysiaodoriphaga Yanget Zhang belongs to Diptera Diptera, Longhorn suborder Nematocera, Sciaridae Sciaridae, Bradysiaodoriphaga Yanget Zhang, and its larvae are commonly known as leek maggots, which are the main pests of leeks. The larvae harm leek leaf sheaths, young buds and bulbs, causing bulbs to rot and leaves to die. The mild ones cause lodging and yellowing, and the severe ones lack seedlings and break ridges, seriously affecting the yield and quality of leeks. [0003] The larvae of Bradysiadifformis, commonly known as maggots, belong to the same genus Bradysiadifformis as Bradysiadifformis. The traditional method of distinguishing chive maggots from fungus maggots is based on their morphological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 褚栋杨峰山郭雅男王磊台术蕾张存环
Owner QINGDAO AGRI UNIV
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