Molecular identification primers and application methods of four fruit borer moths

Abstract

The invention discloses a molecular identification primer for four fruit tree core-eating insects and also discloses a sequence of the molecular identification primer and a method for using the molecular identification prime. According to the primer used for identifying four fruit tree core-eating insects such as Cydia pomonella, Grapholitha molesta Busck, Grapholitha dimorpha Komai and Grapholitha funebrana Treitscheke, mitochondria COI and COII genes of the four fruit tree core-eating insects can be amplified, the core-eating insect which has over 99 percent of homology with the provided tree core-eating insect sequence is the tree core-eating insect, a simple and stable molecular marker is provided for identifying the four core-eating insects, and the problem that the four core-eating insects cannot be distinguished and identified is solved. Moreover, the differences of the mitochondria COI and COII genes of the four fruit tree core-eating insects are explored from a molecular level, an identification technology of the four core-eating insects is explored and established, and a foundation is laid for biological and population dynamics monitoring and integrated control of the fruit tree core-eating insects in the future.

Description

technical field [0001] The invention relates to a method for identifying fruit tree diseases and insect pests, in particular to a primer for molecular identification of four fruit tree borer moths and a use method thereof. Background technique [0002] The codling moth Cydiapomonella belongs to Lepidoptera Lepidoptera Tortricidae and Cydia. Its hosts include pome fruits such as apples, apple pears, sand fruits, pears, hawthorns, and crabapples, and stone fruits such as peaches, plums, apricots, and jujubes. Horticultural plants such as pomegranates, cherries, berries, and walnuts are one of the important worldwide fruit tree pests. The insect mainly damages the fruit with larvae and eats the seeds. It has the habit of turning fruit and causing damage. The fruit-eating rate is generally above 50%, and it can reach 100% in severe cases. It often causes a large number of fruit drop, reducing the yield and quality of the fruit. The worm originated in southeastern Europe, and th...

AI Technical Summary

Problems solved by technology

The external morphology of codling moth, pear borer, sand fruit borer and plum borer larvae is very similar, and they often occur in the same orchard at the same time, which makes traditional morphological identification more difficult and less accurate

When using pheromone lures to monitor population dynamics, the sex pheromones of the pear borer can attract some of the fruit borer and the small borer, and the lures of the sand fruit borer can also attract two small pear borers and small plum borers , its adults are also difficult to distinguish and identify, which not only brings difficulties to the forecasting work, but also increases the difficulty of effectively distinguishing them

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