Method for extracting and purifying active polysaccharides of wheat bran

A technology of wheat bran and active polysaccharides, which is applied in allergic diseases, drug combinations, etc., can solve the problems of high extraction cost, unpurified polysaccharides, and low biological activity of crude products, and achieve low operating costs, strong immune regulation activity, The effect of simple extraction method

Active Publication Date: 2014-01-08
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After searching the literature of the prior art, it was found that Chinese patent CN200910237352.8 discloses a wheat bran polysaccharide with anti-tumor and immunoregulatory activities and its extraction method, which uses xylanase to enzymatically hydrolyze wheat bran to obtain Arabinoxylan (or pentosan)-based polysaccharide products, but the cost of enzyme extraction is high, not suitable for large-scale production applications, and the polysaccharides have not been purified, and the crude product has low biological activity

Method used

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  • Method for extracting and purifying active polysaccharides of wheat bran
  • Method for extracting and purifying active polysaccharides of wheat bran
  • Method for extracting and purifying active polysaccharides of wheat bran

Examples

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Embodiment 1

[0050] The extraction and purification method of wheat bran active polysaccharide, its further optimized operation steps are as follows:

[0051] a. Pretreatment: remove impurities from wheat bran, dry at 60°C, and grind to 40-50 mesh;

[0052] b. Degreasing:

[0053] (1) Ethanol degreasing:

[0054] Immerse the wheat bran powder treated in step a in ethanol with 6 times its weight and 85% purity, heat and reflux at 75°C and stir for 2.5h, cool to room temperature and continue stirring for 16h, remove the liquid, and add 10% of its weight twice. times, the purity is 85% ethanol, stirred at room temperature for 6h, and removed the liquid;

[0055] (2) Acetone degreasing:

[0056] Add an acetone solution of 3 times its weight and 99% purity to the wheat bran after ethanol degreasing, remove the liquid after stirring for 30 minutes, and naturally volatilize and dry at room temperature for 12 hours to obtain defatted bran;

[0057] c. Polysaccharide extraction:

[0058] Add 24 ...

Embodiment 2

[0069] The extraction and purification method of wheat bran active polysaccharide, its further optimized operation steps are as follows:

[0070] a. Pretreatment: remove impurities from wheat bran, dry at 65°C, and grind to 40-50 mesh;

[0071] b. Degreasing:

[0072] (1) Ethanol degreasing:

[0073] Immerse the wheat bran powder treated in step a in ethanol with a purity of 85% and 10 times its weight, heat and reflux at 65°C for 2 hours, cool to room temperature and continue stirring for 12 hours, remove the liquid, and add 13 times its weight twice 1. Purity is 85% ethanol, stir at room temperature for 4h, remove liquid;

[0074] (2) Acetone degreasing:

[0075] Add an acetone solution with 6 times its weight and a purity of 99% to the wheat bran after ethanol defatting, remove the liquid after stirring for 30 minutes, and naturally volatilize and dry at room temperature for 12 hours to obtain defatted bran;

[0076] c. Polysaccharide extraction:

[0077] Add the defat...

Embodiment 3

[0088] The extraction and purification method of wheat bran active polysaccharide, its further optimized operation steps are as follows:

[0089] a. Pretreatment: remove impurities from wheat bran, dry at 60°C-65°C, and grind to 40-50 mesh;

[0090] b. Degreasing:

[0091] (1) Ethanol degreasing:

[0092] Immerse the wheat bran powder treated in step a in ethanol with a purity of 85% of its weight, heat and reflux at 68°C to 72°C for 2.3h, cool to room temperature and continue stirring for 14h, remove the liquid, and add Its weight 12 times, the ethanol that purity is 85%, stir 5h at room temperature, remove liquid;

[0093] (2) Acetone degreasing:

[0094] Add an acetone solution of 5 times its weight and 99% purity to the wheat bran after ethanol degreasing, remove the liquid after stirring for 30 minutes, and naturally volatilize and dry at room temperature for 12 hours to obtain defatted bran;

[0095] c. Polysaccharide extraction:

[0096] Add 26 times the weight of ...

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Abstract

The invention belongs to the technical field of deep wheat bran processing, and in particular relates to a method for extracting and purifying active polysaccharides of wheat bran. The method comprises the following steps: carrying out pretreatment, degreasing, polysaccharide extraction and crude polysaccharide refining; analyzing the yield, chemical composition and immunoregulatory activity of extracted crude products and purified components. By virtue of the method for extracting and purifying the active polysaccharides of the wheat bran, crude wheat bran polysaccharide products are prepared through a water extraction and alcohol precipitation method; the polysaccharides with the immunoregulatory activity are obtained by purifying a sepharose gel column. An extraction method is simple and the operation cost is low. The yield of the obtained polysaccharides of the wheat bran is 5.0 percent, the yield of the purified active polysaccharides of the wheat bran is 5.6 percent, and the polysaccharides have high immunoregulatory activity.

Description

technical field [0001] The invention belongs to the technical field of deep processing of wheat bran, in particular to a method for extracting and purifying wheat bran active polysaccharides. Background technique [0002] Wheat bran is the residual part after wheat flour and germ are extracted in the process of wheat flour making. It is the main processing by-product of wheat flour mill. The germ and the unstripped endosperm usually account for 14% to 19% of the weight of wheat. It contains non-starch polysaccharides, starch, fat, lignin, protein, vitamins, phenolic acid compounds and other components. At present, except for a small amount of wheat bran, wheat bran dietary fiber, xylitol and ferulic acid extraction, wheat bran is mainly used as feed, the economic benefit is not high, and the non-starch polysaccharide content in wheat bran is about 46%. Therefore, it is very necessary to study the method of extracting and purifying polysaccharides from wheat bran to improve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/02
Inventor 曹荣安贾建李良玉张建强鹿保鑫刘妍妍张丽萍杨楠郭希娟
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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